Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PotMrB4_RS05675 Genome accession   NZ_AP022642
Coordinates   1199733..1200950 (-) Length   405 a.a.
NCBI ID   WP_172432749.1    Uniprot ID   A0A679GDV3
Organism   Metapseudomonas otitidis strain MrB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1194733..1205950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS05630 (PtoMrB4_11200) - 1195137..1195310 (-) 174 WP_044406847.1 DUF3094 family protein -
  PotMrB4_RS05635 (PtoMrB4_11210) - 1195390..1196016 (-) 627 WP_172435045.1 DUF1780 domain-containing protein -
  PotMrB4_RS05640 (PtoMrB4_11220) - 1196136..1196561 (+) 426 WP_107331796.1 GNAT family N-acetyltransferase -
  PotMrB4_RS05645 (PtoMrB4_11230) - 1196558..1197034 (+) 477 WP_052807598.1 hypothetical protein -
  PotMrB4_RS05650 (PtoMrB4_11240) - 1197031..1197717 (+) 687 WP_142011982.1 energy-coupling factor ABC transporter permease -
  PotMrB4_RS05655 (PtoMrB4_11250) - 1197828..1198043 (+) 216 WP_160481384.1 hypothetical protein -
  PotMrB4_RS05660 (PtoMrB4_11260) yacG 1198056..1198253 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  PotMrB4_RS05665 (PtoMrB4_11270) coaE 1198250..1198861 (-) 612 WP_069559818.1 dephospho-CoA kinase -
  PotMrB4_RS05670 (PtoMrB4_11280) pilD 1198858..1199730 (-) 873 WP_172432748.1 A24 family peptidase Machinery gene
  PotMrB4_RS05675 (PtoMrB4_11290) pilC 1199733..1200950 (-) 1218 WP_172432749.1 type II secretion system F family protein Machinery gene
  PotMrB4_RS05680 (PtoMrB4_11300) pilB 1200954..1202657 (-) 1704 WP_172432750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PotMrB4_RS05685 (PtoMrB4_11310) pilE 1202881..1203363 (+) 483 WP_172432751.1 pilin Machinery gene
  PotMrB4_RS05690 - 1203383..1205113 (+) 1731 WP_172432752.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44012.69 Da        Isoelectric Point: 9.9318

>NTDB_id=79059 PotMrB4_RS05675 WP_172432749.1 1199733..1200950(-) (pilC) [Metapseudomonas otitidis strain MrB4]
MADKALKTSVFKWEGTDKKGTKVTGEISGSNPALVKAQLRKQGINPTKVRKKAASLFSKNKKIKPLEIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNASMRKLVDDVKQDVAAGNSFASSLRKRPIYFDDLYCNLVDSGEQAGALETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVSMILLIKVVPQFEAVFSNFGAELPAFTLMVIGLSEFLQEWWWLVILGLFGTAFGLK
QVHQKSEKFRNSVDRAALKAPIVGNIIYKSAVARFARTLATTFAAGVPLVDALDSVSGATGNIVFKQAVNKVKQDVSTGM
QLNFSMRTTGVFPTMAIQMTAIGEESGALDSMLDKLATFYEEEVDNAVDGLTSLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=79059 PotMrB4_RS05675 WP_172432749.1 1199733..1200950(-) (pilC) [Metapseudomonas otitidis strain MrB4]
ATGGCGGATAAGGCATTAAAGACCAGCGTCTTCAAGTGGGAAGGCACTGACAAGAAAGGTACCAAGGTAACGGGTGAGAT
CAGCGGCTCCAATCCGGCCCTTGTCAAAGCTCAACTCCGTAAGCAAGGTATCAATCCAACCAAGGTTCGAAAAAAGGCTG
CCTCACTCTTCAGCAAAAACAAGAAAATCAAGCCACTAGAAATCGCCTTATTTACACGTCAGATGGCGACAATGATGAAG
GCAGGCGTCCCTCTTCTACAGAGCTTCGACATTATCGGGGAAGGCTTTGATAATGCCAGCATGCGAAAACTGGTCGATGA
TGTTAAGCAAGATGTAGCAGCGGGTAACAGCTTTGCCTCTTCGTTGCGAAAACGCCCGATCTATTTCGACGATCTTTATT
GCAACTTGGTGGACTCAGGCGAGCAAGCCGGCGCACTTGAAACCTTGCTTGACCGCGTAGCAACTTACAAGGAAAAAACA
GAAGCCCTCAAAGCCAAAATAAAAAAGGCGATGAATTACCCCATCGCAGTAGTGGTAGTTGCAATTATCGTTTCAATGAT
CCTTCTGATTAAAGTTGTACCGCAATTTGAAGCTGTATTTAGTAACTTCGGTGCCGAGCTGCCTGCATTCACTCTGATGG
TGATCGGGCTTTCCGAATTCCTCCAAGAGTGGTGGTGGCTGGTAATTTTGGGGCTGTTTGGCACAGCTTTTGGACTGAAG
CAAGTTCACCAGAAATCCGAGAAATTTCGTAATAGCGTGGACCGGGCAGCACTCAAAGCCCCTATTGTCGGTAACATCAT
CTACAAATCGGCAGTAGCTCGCTTTGCACGAACCCTTGCGACTACGTTTGCGGCTGGTGTCCCGTTGGTTGATGCGCTTG
ACTCTGTATCCGGAGCGACTGGCAACATCGTATTCAAACAAGCGGTCAACAAAGTCAAGCAAGATGTGTCCACAGGCATG
CAGCTCAATTTCTCCATGCGTACCACGGGGGTCTTCCCAACCATGGCAATCCAGATGACTGCTATCGGCGAAGAGTCAGG
CGCACTAGACTCCATGTTGGATAAATTGGCCACCTTTTACGAAGAAGAAGTCGATAACGCTGTTGATGGCCTGACTTCAC
TGATGGAGCCGCTGATCATGTCTGTCTTGGGTGTTCTGGTCGGCGGTCTCATCATTGCCATGTACCTCCCTATCTTCCAA
CTCGGCTCCGTAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679GDV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.247

100

0.802
  pilC Acinetobacter baylyi ADP1

58.867

100

0.59
  pilC Acinetobacter baumannii D1279779

58.168

99.753

0.58
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.284

99.259

0.43
  pilG Neisseria meningitidis 44/76-A

43.035

99.259

0.427
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

42.172

97.778

0.412
  pilC Thermus thermophilus HB27

36.538

100

0.375

Multiple sequence alignment