Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PotMrB4_RS05680 Genome accession   NZ_AP022642
Coordinates   1200954..1202657 (-) Length   567 a.a.
NCBI ID   WP_172432750.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain MrB4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1195954..1207657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS05640 (PtoMrB4_11220) - 1196136..1196561 (+) 426 WP_107331796.1 GNAT family N-acetyltransferase -
  PotMrB4_RS05645 (PtoMrB4_11230) - 1196558..1197034 (+) 477 WP_052807598.1 hypothetical protein -
  PotMrB4_RS05650 (PtoMrB4_11240) - 1197031..1197717 (+) 687 WP_142011982.1 energy-coupling factor ABC transporter permease -
  PotMrB4_RS05655 (PtoMrB4_11250) - 1197828..1198043 (+) 216 WP_160481384.1 hypothetical protein -
  PotMrB4_RS05660 (PtoMrB4_11260) yacG 1198056..1198253 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  PotMrB4_RS05665 (PtoMrB4_11270) coaE 1198250..1198861 (-) 612 WP_069559818.1 dephospho-CoA kinase -
  PotMrB4_RS05670 (PtoMrB4_11280) pilD 1198858..1199730 (-) 873 WP_172432748.1 A24 family peptidase Machinery gene
  PotMrB4_RS05675 (PtoMrB4_11290) pilC 1199733..1200950 (-) 1218 WP_172432749.1 type II secretion system F family protein Machinery gene
  PotMrB4_RS05680 (PtoMrB4_11300) pilB 1200954..1202657 (-) 1704 WP_172432750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PotMrB4_RS05685 (PtoMrB4_11310) pilE 1202881..1203363 (+) 483 WP_172432751.1 pilin Machinery gene
  PotMrB4_RS05690 - 1203383..1205113 (+) 1731 WP_172432752.1 PglL family O-oligosaccharyltransferase -
  PotMrB4_RS05700 (PtoMrB4_11330) nadC 1205294..1206142 (-) 849 WP_172432753.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62500.59 Da        Isoelectric Point: 5.3455

>NTDB_id=79060 PotMrB4_RS05680 WP_172432750.1 1200954..1202657(-) (pilB) [Metapseudomonas otitidis strain MrB4]
MNDSVNLTGLAKQMVIANLLDEKTASQAQQQAARNKLSLVTYLVQNKLVKSRDLIELAAEQFGVAYVDLNSIDRDSYPKE
LVSEKLIRQHRVLPLWRRGNKLFIAVSDPANHQAVTDIQFNTGLTTESILVEDDKLGVAIDKFFEGEASGLDDLGDVDLD
GLDVEAVDDDKGSADAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERSYRVRFRTDGILHEVARPPVQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEDFQKELYLEALKQPQ
GMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCGTCKKEVDVPRDTLLKEGFP
ESKIGTFKIFGPVGCENCKGGYKGRVGIYEVVKITPALQRIIMEEGNSIDIATQMRKDGFNDLRTSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=79060 PotMrB4_RS05680 WP_172432750.1 1200954..1202657(-) (pilB) [Metapseudomonas otitidis strain MrB4]
ATGAATGACAGCGTAAACCTGACAGGCTTGGCCAAGCAGATGGTCATTGCCAATCTATTAGACGAAAAAACAGCCTCACA
GGCACAGCAACAGGCAGCTCGCAACAAACTCTCTCTGGTGACCTATCTGGTTCAGAACAAGCTTGTGAAGAGTCGCGATC
TGATTGAGTTAGCTGCCGAGCAGTTCGGTGTCGCCTATGTTGATCTCAACTCGATCGACAGGGACAGCTATCCCAAGGAA
CTCGTCAGTGAAAAGCTCATACGCCAGCATAGAGTGCTGCCGCTATGGCGTCGGGGCAACAAGCTCTTCATAGCCGTATC
GGACCCCGCCAACCACCAAGCCGTTACAGACATTCAGTTCAACACCGGCCTCACTACCGAATCGATCCTGGTTGAAGATG
ACAAACTGGGCGTTGCAATCGATAAATTTTTTGAAGGTGAAGCATCCGGGCTGGACGACTTGGGAGATGTCGACCTAGAT
GGCTTGGATGTCGAAGCGGTAGACGATGACAAAGGCTCAGCCGATGCTGGCCAGGATGCAGATGATGCGCCAGTAGTACG
CTTCGTCAACAAGATGCTACTCGATGCGATCAAGGGAGGATCTTCCGACCTGCACTTCGAACCCTATGAAAGGAGCTACA
GGGTTCGCTTCCGAACTGATGGAATCCTTCACGAAGTGGCTAGACCCCCTGTCCAGCTTGCAAGTCGGATATCTGCACGA
CTCAAGGTGATGGCCGCGCTGGACATCTCTGAACGTCGTAAGCCTCAGGACGGGCGGATCAAGATGAAGATTTCCAAGAC
CAAGGCGATTGACTTTCGAGTCAACACCCTTCCCACCCTGTGGGGAGAAAAAATCGTCATGCGGATCCTCGACCCCACGA
GTGCTCAGATGGGGATCGATGCTCTGGGTTACGAAGACTTCCAGAAAGAGCTGTACCTAGAGGCCCTGAAGCAACCCCAA
GGCATGATTCTGGTGACCGGCCCTACCGGCTCCGGCAAAACCGTATCCCTCTATACAGGCATTAATATCCTCAATACGGT
AGACATCAATATCTCCACCGCAGAGGACCCAGTGGAGATCAACCTGGAAGGGATCAACCAGGTAAACGTGAACCCTCGAC
AGGGCATGGATTTCGCTCAAGCGCTACGCGCCTTCCTCCGCCAAGACCCGGATGTGATCATGGTGGGCGAAATTCGGGAC
CTTGAGACAGCAGAAATCGCTATCAAGGCCGCTCAGACCGGCCACATGGTGATGTCAACGCTCCACACCAACAGCGCAGC
AGAGACCCTGACGCGTCTCCGCAACATGGGAGTGCCCGCGTTCAACATCGCCACCTCGGTCAACCTGATCATTGCGCAAC
GCTTGGCGCGAAAGCTCTGTGGCACTTGTAAGAAGGAAGTGGATGTGCCCCGTGACACCTTGCTAAAAGAGGGTTTTCCG
GAGAGCAAGATCGGTACGTTCAAGATTTTCGGGCCGGTCGGCTGCGAAAATTGCAAAGGTGGCTACAAAGGCAGGGTAGG
TATTTATGAAGTAGTTAAAATCACGCCTGCCTTGCAACGCATTATCATGGAGGAAGGTAACTCCATCGATATCGCAACAC
AGATGCGCAAGGATGGGTTCAATGACCTGCGTACCTCGGGCCTGCTCAAAGCCATGCAGGGCGTTACCAGCCTCGAGGAA
GTCAACCGAGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.123

100

0.594
  pilB Acinetobacter baylyi ADP1

57.549

99.295

0.571
  pilB Legionella pneumophila strain ERS1305867

56.261

100

0.563
  pilB Vibrio campbellii strain DS40M4

49.468

99.471

0.492
  pilB Vibrio cholerae strain A1552

49.643

98.765

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

50.464

95.062

0.48
  pilF Neisseria gonorrhoeae MS11

50.092

95.767

0.48
  pilF Thermus thermophilus HB27

36.738

98.413

0.362

Multiple sequence alignment