Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUN50_RS02430 Genome accession   NZ_CP117988
Coordinates   508700..509806 (+) Length   368 a.a.
NCBI ID   WP_005425812.1    Uniprot ID   A7MTP8
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503700..514806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS02395 (PUN50_02395) - 503840..504442 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  PUN50_RS02400 (PUN50_02400) - 504548..504979 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  PUN50_RS02405 (PUN50_02405) yggU 505039..505329 (-) 291 WP_162093856.1 DUF167 family protein YggU -
  PUN50_RS02410 (PUN50_02410) - 505329..505886 (-) 558 WP_005425806.1 YggT family protein -
  PUN50_RS02415 (PUN50_02415) proC 505940..506758 (-) 819 WP_010650285.1 pyrroline-5-carboxylate reductase -
  PUN50_RS02420 (PUN50_02420) - 506889..507599 (-) 711 WP_274290817.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUN50_RS02425 (PUN50_02425) pilT 507628..508668 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  PUN50_RS02430 (PUN50_02430) pilU 508700..509806 (+) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUN50_RS02435 (PUN50_02435) ruvX 509868..510293 (-) 426 WP_038892069.1 Holliday junction resolvase RuvX -
  PUN50_RS02440 (PUN50_02440) - 510341..510904 (-) 564 WP_038892068.1 YqgE/AlgH family protein -
  PUN50_RS02445 (PUN50_02445) gshB 511034..511984 (-) 951 WP_005425823.1 glutathione synthase -
  PUN50_RS02450 (PUN50_02450) rsmE 511999..512730 (-) 732 WP_274290818.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUN50_RS02455 (PUN50_02455) endA 512877..513572 (-) 696 WP_005425828.1 deoxyribonuclease I -
  PUN50_RS02460 (PUN50_02460) - 513723..514220 (-) 498 WP_012128940.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.48 Da        Isoelectric Point: 6.7731

>NTDB_id=788951 PUN50_RS02430 WP_005425812.1 508700..509806(+) (pilU) [Vibrio campbellii strain 20220413_1]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=788951 PUN50_RS02430 WP_005425812.1 508700..509806(+) (pilU) [Vibrio campbellii strain 20220413_1]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGCGAATTGCGCCCGCAAGGCGATAAGCTGACTGAAAATGATGTCGCCTTGTTACTCGACAGTGCCA
TGGAACCAGAGCGACGCCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGACCTTGCTATTGCCAAGCGTGGCTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTATCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAACATAAGCGTTGCATCGTGACACAGCGTGAAGTGGGCTTAGACACCGACAGCTATGAAGTGGCGTT
GAAAAATTCGTTACGTCAGGCGCCAGACATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTGGAGCGTATTTTGCAC
CTAGTGCCAAAAGATCAGAAAGATCAGTTCTTATTTGATCTGTCGATGAACTTAAAAGGTGTGGTGGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAACAGTCCACGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAATTGAAATCGACCATGGCTCGCTCTAACGAGTTCGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATTAGCGAAGAGGATGCATTGCACAGTGCAGATTCAGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACAGGCACGCTGGCGAATGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.25

100

0.813
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375