Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQO03_RS11385 Genome accession   NZ_CP117811
Coordinates   2770918..2772000 (+) Length   360 a.a.
NCBI ID   WP_274150384.1    Uniprot ID   -
Organism   Lentisphaera profundi strain SAORIC-696     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765918..2777000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQO03_RS11370 (PQO03_11370) - 2766308..2767591 (-) 1284 WP_274150382.1 hypothetical protein -
  PQO03_RS11375 (PQO03_11375) - 2767962..2769176 (+) 1215 WP_274149601.1 IS4 family transposase -
  PQO03_RS11380 (PQO03_11380) - 2769475..2770908 (+) 1434 WP_274150383.1 type IV pilus twitching motility protein PilT -
  PQO03_RS11385 (PQO03_11385) pilT 2770918..2772000 (+) 1083 WP_274150384.1 type IV pilus twitching motility protein PilT Machinery gene
  PQO03_RS11390 (PQO03_11390) - 2772127..2772795 (-) 669 WP_274150385.1 MBL fold metallo-hydrolase -
  PQO03_RS11395 (PQO03_11395) - 2772788..2774515 (-) 1728 WP_274150386.1 ABC transporter ATP-binding protein -
  PQO03_RS11400 (PQO03_11400) - 2774512..2776353 (-) 1842 WP_274150387.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39701.12 Da        Isoelectric Point: 6.8804

>NTDB_id=787625 PQO03_RS11385 WP_274150384.1 2770918..2772000(+) (pilT) [Lentisphaera profundi strain SAORIC-696]
MAARMDGFLHYLFDKGGSDLHLLSNHVPKIRKSGNLVAMEGEGVIGADEMEGILKEICPDAKWKQYVENFDLDFAYEIPG
VARFRVNYMRTVDGMASVMRTIPTEILTMEQLNLPDTFKDVVEQGPGLILVTGPTGSGKSTTLAAMINHINETQSKHIIT
IEDPLEFVHPNKKSIIVQREVHDHTTSFNMALKGAMRADPDIVLVGEMRDPETIGLALSCAAMGLMVFGTLHTNNAPKTI
DRIIGAFPAKQQPQVRAMLASSLRTIISQLLCKTTDGKRCASHEILLFHPALPGLIREGKLSSIRGLIEGGVKKGMKTMD
MDLLRLYKEGRISGEEAYMKAAEKVTFQDFLPPGYFGDKT

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=787625 PQO03_RS11385 WP_274150384.1 2770918..2772000(+) (pilT) [Lentisphaera profundi strain SAORIC-696]
ATGGCAGCAAGAATGGATGGTTTTTTGCACTACCTCTTTGATAAGGGTGGATCAGATTTACACTTATTGAGTAATCACGT
ACCTAAAATCCGCAAAAGTGGTAACCTGGTGGCAATGGAAGGAGAAGGTGTCATTGGTGCAGACGAAATGGAAGGGATTT
TAAAAGAAATCTGCCCCGATGCAAAATGGAAGCAATATGTTGAGAATTTTGACCTGGATTTTGCCTATGAAATCCCGGGA
GTTGCTCGTTTTCGTGTGAACTATATGCGTACCGTAGATGGTATGGCATCGGTGATGCGTACGATTCCGACAGAAATTTT
AACTATGGAACAATTAAACCTACCAGATACCTTTAAAGATGTGGTTGAACAGGGACCTGGTCTGATTTTAGTGACAGGAC
CTACGGGTTCGGGTAAATCGACGACTTTGGCGGCAATGATTAATCATATTAATGAGACCCAAAGTAAACATATCATTACG
ATTGAAGATCCACTAGAATTCGTTCACCCTAATAAAAAAAGTATTATTGTGCAGCGAGAAGTTCACGATCATACGACTTC
ATTTAATATGGCTCTAAAAGGGGCGATGCGTGCCGACCCTGATATTGTCCTTGTTGGTGAAATGCGAGATCCGGAAACGA
TTGGTTTGGCACTCTCTTGTGCGGCCATGGGGCTGATGGTATTTGGAACCTTGCACACCAATAATGCGCCTAAAACGATT
GACCGTATTATCGGTGCTTTCCCAGCAAAGCAACAACCTCAGGTTCGTGCGATGTTAGCAAGTAGTCTGCGTACTATTAT
TTCGCAGTTGCTCTGTAAGACGACTGATGGTAAACGCTGTGCCTCTCATGAAATTCTACTGTTTCACCCGGCATTACCTG
GTTTGATCCGCGAGGGTAAATTAAGTAGTATTCGTGGCCTCATTGAGGGTGGCGTTAAGAAGGGTATGAAAACAATGGAT
ATGGATTTATTACGCCTCTATAAAGAGGGACGTATTTCTGGTGAAGAAGCCTATATGAAAGCTGCTGAAAAAGTGACTTT
TCAAGATTTCTTACCACCAGGGTACTTCGGTGATAAAACATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

51.796

92.778

0.481
  pilT Neisseria meningitidis 8013

51.497

92.778

0.478
  pilT Acinetobacter nosocomialis M2

48.406

95.833

0.464
  pilT Acinetobacter baumannii D1279779

48.406

95.833

0.464
  pilT Acinetobacter baumannii strain A118

48.406

95.833

0.464
  pilT Pseudomonas aeruginosa PAK

48.378

94.167

0.456
  pilT Legionella pneumophila strain Lp02

47.536

95.833

0.456
  pilT Legionella pneumophila strain ERS1305867

47.536

95.833

0.456
  pilT Pseudomonas stutzeri DSM 10701

47.246

95.833

0.453
  pilT Acinetobacter baylyi ADP1

47.246

95.833

0.453
  pilT Vibrio cholerae strain A1552

47.147

92.5

0.436
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.147

92.5

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.988

94.722

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.412

94.444

0.372