Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PSH97_RS23760 Genome accession   NZ_CP117454
Coordinates   5246017..5246439 (-) Length   140 a.a.
NCBI ID   WP_305446926.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1935     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5241017..5251439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH97_RS23745 (PSH97_23740) - 5241872..5244862 (+) 2991 WP_305446924.1 DUF748 domain-containing protein -
  PSH97_RS23750 (PSH97_23745) - 5244862..5245158 (+) 297 WP_305449860.1 DUF2845 domain-containing protein -
  PSH97_RS23755 (PSH97_23750) - 5245338..5245700 (-) 363 WP_305446925.1 BON domain-containing protein -
  PSH97_RS23760 (PSH97_23755) pilA/pilAI 5246017..5246439 (-) 423 WP_305446926.1 pilin Machinery gene
  PSH97_RS23765 (PSH97_23760) pilB 5246666..5248366 (+) 1701 WP_305446927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH97_RS23770 (PSH97_23765) pilC 5248369..5249586 (+) 1218 WP_305446928.1 type II secretion system F family protein Machinery gene
  PSH97_RS23775 (PSH97_23770) pilD 5249588..5250460 (+) 873 WP_305446929.1 A24 family peptidase Machinery gene
  PSH97_RS23780 (PSH97_23775) coaE 5250457..5251080 (+) 624 WP_305446930.1 dephospho-CoA kinase -
  PSH97_RS23785 (PSH97_23780) yacG 5251077..5251277 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14114.36 Da        Isoelectric Point: 8.1079

>NTDB_id=785540 PSH97_RS23760 WP_305446926.1 5246017..5246439(-) (pilA/pilAI) [Pseudomonas cucumis strain FP1935]
MKNQKGFTLIELLIVVAIIGILATFALPQYSKYQARAKVTAALAEISALKVPFEDIINQGTNPTLALIGGTATTGNCTIT
ASGTAADGAGTIGCVILNAPGPVLSKTITLTRTLTGGWTCATTAEQEYAPKGCTGAAAAG

Nucleotide


Download         Length: 423 bp        

>NTDB_id=785540 PSH97_RS23760 WP_305446926.1 5246017..5246439(-) (pilA/pilAI) [Pseudomonas cucumis strain FP1935]
ATGAAAAATCAAAAAGGTTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGTT
GCCGCAGTATTCGAAATATCAAGCGCGAGCGAAGGTTACCGCTGCTCTTGCAGAAATTTCGGCATTGAAGGTGCCTTTCG
AAGACATTATCAACCAGGGCACTAACCCCACCCTCGCACTGATCGGCGGTACGGCGACCACCGGTAATTGCACGATTACG
GCATCGGGTACCGCCGCTGACGGTGCGGGTACTATTGGTTGCGTTATTCTCAATGCTCCAGGGCCGGTGCTCAGCAAAAC
CATTACGCTTACACGCACCCTTACCGGTGGCTGGACATGCGCCACCACAGCTGAACAGGAATACGCGCCTAAAGGCTGCA
CTGGCGCTGCCGCTGCTGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50

98.571

0.493
  pilA Acinetobacter baumannii strain A118

47.101

98.571

0.464
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  comP Acinetobacter baylyi ADP1

40.132

100

0.436
  pilA Vibrio cholerae C6706

42.069

100

0.436
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.069

100

0.436
  pilA Vibrio cholerae strain A1552

42.069

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila strain ERS1305867

40.972

100

0.421
  pilA2 Legionella pneumophila str. Paris

40.278

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

36.957

98.571

0.364