Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH97_RS23770 Genome accession   NZ_CP117454
Coordinates   5248369..5249586 (+) Length   405 a.a.
NCBI ID   WP_305446928.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1935     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5243369..5254586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH97_RS23750 (PSH97_23745) - 5244862..5245158 (+) 297 WP_305449860.1 DUF2845 domain-containing protein -
  PSH97_RS23755 (PSH97_23750) - 5245338..5245700 (-) 363 WP_305446925.1 BON domain-containing protein -
  PSH97_RS23760 (PSH97_23755) pilA/pilAI 5246017..5246439 (-) 423 WP_305446926.1 pilin Machinery gene
  PSH97_RS23765 (PSH97_23760) pilB 5246666..5248366 (+) 1701 WP_305446927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH97_RS23770 (PSH97_23765) pilC 5248369..5249586 (+) 1218 WP_305446928.1 type II secretion system F family protein Machinery gene
  PSH97_RS23775 (PSH97_23770) pilD 5249588..5250460 (+) 873 WP_305446929.1 A24 family peptidase Machinery gene
  PSH97_RS23780 (PSH97_23775) coaE 5250457..5251080 (+) 624 WP_305446930.1 dephospho-CoA kinase -
  PSH97_RS23785 (PSH97_23780) yacG 5251077..5251277 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  PSH97_RS23790 (PSH97_23785) - 5251287..5251502 (-) 216 WP_007938959.1 hypothetical protein -
  PSH97_RS23795 (PSH97_23790) - 5251566..5252255 (-) 690 WP_305446931.1 energy-coupling factor ABC transporter permease -
  PSH97_RS23800 (PSH97_23795) - 5252502..5253956 (+) 1455 WP_305446932.1 TrkH family potassium uptake protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44137.10 Da        Isoelectric Point: 9.9046

>NTDB_id=785542 PSH97_RS23770 WP_305446928.1 5248369..5249586(+) (pilC) [Pseudomonas cucumis strain FP1935]
MAVKAAKISVYAWEGTDKKGTKMTGELTGQNPALIKAQLRKQGINPGKVRKKTASIFSAGKRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFSGFGAELPAFTVMVIGLSEFLQAWWWMVLGMLGALVFGVR
HAFKKSPGFRDRMDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFAMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=785542 PSH97_RS23770 WP_305446928.1 5248369..5249586(+) (pilC) [Pseudomonas cucumis strain FP1935]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTGTATGCCTGGGAAGGCACAGACAAGAAAGGCACAAAAATGACCGGCGAGTT
GACCGGCCAGAATCCCGCGCTGATCAAGGCCCAGTTACGCAAGCAAGGGATCAACCCGGGCAAGGTGCGGAAAAAAACCG
CGTCCATTTTCAGTGCCGGAAAACGCATCAAGCCCCTGGACATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTGCTGCAATCGTTCGACATTATCGGCGAGGGCTTCGATAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCGGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAAAAGCCCCAGTATTTCGATGAGCTGTACT
GCAACCTGGTGGATGCCGGCGAGCAGGCTGGCGCCCTGGATACCCTGCTGGAACGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACTTACCCGCTGGCGGTGGTGTTCGTCGCGATCATTGTGACCGGGAT
CCTGCTGGTCAAGGTTGTGCCGCAATTCGAGTCAGTGTTCTCGGGGTTTGGCGCCGAGCTGCCGGCGTTCACCGTGATGG
TCATCGGTTTGTCGGAGTTCCTGCAGGCCTGGTGGTGGATGGTGCTCGGCATGCTGGGTGCGCTGGTCTTCGGCGTCCGC
CATGCCTTTAAAAAGTCCCCGGGCTTTCGGGACCGGATGGACACCTGGCTGCTGAAACTGCCGTTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCTTGTCGACGACGTTTGCCGCTGGGGTGCCACTGGTCGAAGCCCTGG
AGTCAGTGGCGGGCGCGACTGGCAACATCGTGTTCAAGCGAGCGGTGTTACGCATCAAGCAGGACGTTTCAACTGGCATG
CAGCTGAATTTTGCCATGCGCACCTCCGGCATCTTTCCGAACATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCCGG
CGCACTGGACGACATGCTCGACAAGGTCGCAAGTTTCTATGAGGACGAAGTGGATAACATGGTCGATAACCTGACCAGTC
TGATGGAGCCTTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGCCTCGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.631

100

0.748
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.523

100

0.417
  pilC Vibrio cholerae strain A1552

42.424

97.778

0.415
  pilC Thermus thermophilus HB27

38.653

99.012

0.383