Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH97_RS23765 Genome accession   NZ_CP117454
Coordinates   5246666..5248366 (+) Length   566 a.a.
NCBI ID   WP_305446927.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1935     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5241666..5253366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH97_RS23745 (PSH97_23740) - 5241872..5244862 (+) 2991 WP_305446924.1 DUF748 domain-containing protein -
  PSH97_RS23750 (PSH97_23745) - 5244862..5245158 (+) 297 WP_305449860.1 DUF2845 domain-containing protein -
  PSH97_RS23755 (PSH97_23750) - 5245338..5245700 (-) 363 WP_305446925.1 BON domain-containing protein -
  PSH97_RS23760 (PSH97_23755) pilA/pilAI 5246017..5246439 (-) 423 WP_305446926.1 pilin Machinery gene
  PSH97_RS23765 (PSH97_23760) pilB 5246666..5248366 (+) 1701 WP_305446927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH97_RS23770 (PSH97_23765) pilC 5248369..5249586 (+) 1218 WP_305446928.1 type II secretion system F family protein Machinery gene
  PSH97_RS23775 (PSH97_23770) pilD 5249588..5250460 (+) 873 WP_305446929.1 A24 family peptidase Machinery gene
  PSH97_RS23780 (PSH97_23775) coaE 5250457..5251080 (+) 624 WP_305446930.1 dephospho-CoA kinase -
  PSH97_RS23785 (PSH97_23780) yacG 5251077..5251277 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  PSH97_RS23790 (PSH97_23785) - 5251287..5251502 (-) 216 WP_007938959.1 hypothetical protein -
  PSH97_RS23795 (PSH97_23790) - 5251566..5252255 (-) 690 WP_305446931.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62920.99 Da        Isoelectric Point: 5.8242

>NTDB_id=785541 PSH97_RS23765 WP_305446927.1 5246666..5248366(+) (pilB) [Pseudomonas cucumis strain FP1935]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRNRISLVSYLVQNKLLKSLQVAEVASEHFGMALLDLNCLDKDTQPRGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEEDKLSDAIEKFFDNHSTGLEDMADVDLDG
LDIESVDDQKQDSIVGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKIYRVRMRTDGMLREVARPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAHMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNSGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785541 PSH97_RS23765 WP_305446927.1 5246666..5248366(+) (pilB) [Pseudomonas cucumis strain FP1935]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTACTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
GTATCAACAAGCCCAACGCAATCGCATTTCGCTGGTCAGCTATCTGGTGCAAAACAAACTGCTGAAAAGCTTACAGGTCG
CCGAGGTGGCCTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGACAAAGACACTCAACCCCGAGGGCTG
GTCAGTGAGAAACTGATCCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGTGGTAACAAGCTGTTCGTGGGGATTTCCGA
CCCGACCAATCACCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGAAGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGACAACCACTCCACCGGTCTGGAAGACATGGCCGATGTCGATCTCGACGGG
CTGGACATTGAGTCGGTCGATGACCAGAAACAGGATTCAATCGTAGGGCAAGATGCCGATGATGCCCCCGTGGTGCGCTT
CGTCCACAAGATGCTGCTCGACGCGATCAAGAGCGGTTCTTCCGACCTGCATTTCGAACCCTATGAAAAAATCTACCGCG
TGCGCATGCGCACCGATGGCATGCTGCGGGAGGTCGCCAGGCCGCCGACACAACTGGCCAACCGCATTGCTTCCCGGCTG
AAGGTCATGGCCAGTCTCGACATTTCCGAGCGGCGCAAACCCCAGGACGGGCGAATCAAGATGCGCCTGTCCAAAAGCAA
GTCCATCGACTTCCGGGTCAACACACTGCCCACCTTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCCTCCAGTG
CCCATATGGGCATCGATGCCCTCGGCTACGAACCCGATCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGG
ATGATCCTGGTGACTGGTCCCACCGGCTCAGGCAAGACCGTGTCGCTCTATACCGGGCTGAACATTCTCAACACCGTCGA
CATCAATATTTCCACTGCCGAAGACCCGGTAGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGAGGCAGG
GACTGGATTTCGCCCAGGCGCTACGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGCGACCTC
GAAACCGCTGAAATCGCCATCAAGGCTGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGGTTCAACATCGCGACCTCTGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGAAAACTGTGCAGTCATTGCAAAAAGCCTATCGAGATTCCCCGCGAGGCACTGCTCAAAGAAGGCTTTCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCAGTGCAACAGCGGTTACAAAGGGCGAGTAGGGAT
TTATGAAGTGGTAAAGAACACCCCAGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACATCCAGA
TGCGTAAGGACGGCTTCAACGACCTGCGTACTTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AATCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

99.647

0.583
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.101

98.233

0.482
  pilF Thermus thermophilus HB27

36.572

100

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36