Detailed information NTG0785533

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH97_RS16280
• Genome accession: NZ_CP117454
• Coordinates: 3612408..3613517 (-)
• Length: 369 a.a.
• NCBI ID: WP_305445804.1
• Uniprot ID: -
• Organism: Pseudomonas cucumis strain FP1935
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3607408..3618517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH97_RS16275 (PSH97_16270)	-	3608598..3612260 (-)	3663	WP_305445803.1	hypothetical protein	-
PSH97_RS16280 (PSH97_16275)	pilU	3612408..3613517 (-)	1110	WP_305445804.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH97_RS16285 (PSH97_16280)	-	3613637..3616174 (+)	2538	WP_305445805.1	PAS domain S-box protein	-
PSH97_RS16290 (PSH97_16285)	-	3616241..3616516 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
PSH97_RS16295 (PSH97_16290)	-	3616588..3617520 (-)	933	WP_305445806.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41109.18 Da
• Isoelectric Point: 6.5117

>NTDB_id=785533 PSH97_RS16280 WP_305445804.1 3612408..3613517(-) (pilU) [Pseudomonas cucumis strain FP1935]
MEIDALLQTLASRNGSDLYLSTGAPPSAKFDGVLKSLTEQPFKPGEVAAIAASIMDAEQRLEFDRELEMNLALSLPGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIGDLIRRNELGELKNIMEKSEELGMQTFDSAL
YKLLLEGVISLDEALKYADSINNVRLRFKLDSESAMIGKPSSGEWGLVE

Nucleotide

• Length: 1110 bp

>NTDB_id=785533 PSH97_RS16280 WP_305445804.1 3612408..3613517(-) (pilU) [Pseudomonas cucumis strain FP1935]
ATGGAAATCGATGCACTGTTGCAAACTCTGGCCAGCCGCAATGGCTCCGATCTCTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGATGGTGTGCTCAAATCCCTGACCGAGCAACCATTCAAACCGGGTGAAGTCGCGGCTATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGCGAACTGGAAATGAACCTGGCGCTTTCCCTGCCGGGGGTCGGACGC
TTTCGGCTCAATATCTTCAAGCAGCGTAACGATGTGTCCATCGTGGCGCGCAACATCAAGCTCGACATTCCACGCTTCGA
AGACCTCAAGCTGCCACCGGTGCTGCTCGAAACGGTGATGCTCAAGCAGGGGCTGATATTGTTCGTCGGTTCGACCGGTT
CCGGCAAGTCGACCTCGCTGGCGGCACTGATCGATTACCGCAACCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTCGGCGTAGACACCCGCAGCTTTCATGC
AGCGTTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGCGAGACTATGGAGCACG
CGCTGGCGTTTGCCGATACCGGCCATCTGGTAATTTCCACTTTGCATGCCCACAACGCCAATCAGGCGCTGGACCGCGTG
ATCAACTTCTTCCCCGAAGAACGCCGGACGCAACTGCTCCATGACCTGGGCAATAACCTCAAGGCCTTTGTTTCCCAACG
GCTGGTGCGCACCCTTGATGGACAACGAAGGGCGGCGGTGGAGGTGATGCTGGGAACGCCGACTATTGGCGATCTGATTC
GGCGCAACGAATTGGGCGAGCTAAAAAATATCATGGAGAAGTCGGAAGAATTGGGTATGCAAACTTTCGATTCCGCACTT
TATAAGTTGTTGTTGGAAGGTGTGATTAGTCTTGATGAGGCATTGAAATATGCAGATTCCATTAATAACGTAAGGCTGCG
ATTCAAACTTGATAGTGAGTCGGCAATGATCGGGAAACCGTCTTCTGGGGAGTGGGGGTTGGTTGAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.493	96.206	0.534
pilU	Acinetobacter baylyi ADP1	52.528	96.477	0.507
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Pseudomonas stutzeri DSM 10701	41.983	92.954	0.39
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Pseudomonas aeruginosa PAK	41.124	91.599	0.377
pilT	Acinetobacter baylyi ADP1	39.706	92.141	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.528	91.87	0.363
pilT	Legionella pneumophila strain ERS1305867	40.606	89.431	0.363
pilT	Legionella pneumophila strain Lp02	40.606	89.431	0.363
pilT	Acinetobacter baumannii D1279779	40.303	89.431	0.36
pilT	Acinetobacter baumannii strain A118	40.303	89.431	0.36
pilT	Acinetobacter nosocomialis M2	40.303	89.431	0.36