Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PSH92_RS24130 Genome accession   NZ_CP117451
Coordinates   5508676..5509083 (-) Length   135 a.a.
NCBI ID   WP_122564964.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5503676..5514083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH92_RS24115 (PSH92_24110) - 5504443..5507376 (+) 2934 WP_305468529.1 DUF748 domain-containing protein -
  PSH92_RS24120 (PSH92_24115) - 5507378..5507674 (+) 297 WP_305468531.1 DUF2845 domain-containing protein -
  PSH92_RS24125 (PSH92_24120) - 5508055..5508408 (-) 354 WP_025215523.1 BON domain-containing protein -
  PSH92_RS24130 (PSH92_24125) pilA 5508676..5509083 (-) 408 WP_122564964.1 pilin Machinery gene
  PSH92_RS24135 (PSH92_24130) pilB 5509308..5511008 (+) 1701 WP_122564916.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH92_RS24140 (PSH92_24135) pilC 5511011..5512228 (+) 1218 WP_122564917.1 type II secretion system F family protein Machinery gene
  PSH92_RS24145 (PSH92_24140) pilD 5512230..5513099 (+) 870 WP_305468535.1 A24 family peptidase Machinery gene
  PSH92_RS24150 (PSH92_24145) coaE 5513112..5513735 (+) 624 WP_122564919.1 dephospho-CoA kinase -
  PSH92_RS24155 (PSH92_24150) yacG 5513732..5513938 (+) 207 WP_018609255.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14010.20 Da        Isoelectric Point: 8.4989

>NTDB_id=785449 PSH92_RS24130 WP_122564964.1 5508676..5509083(-) (pilA) [Pseudomonas beijingensis strain FP2034]
MNKQKGFTLIELLIVVAIIGILATFALPQYSRYQARAKATAGLGEISALKVAYEDQMNQGVTPTLALMNAPTTTSNCTIS
VTGTATTAGSIVCTLLNAPASIAGRTITLSRDVNGAWTCASTAPREFLPTSCPGT

Nucleotide


Download         Length: 408 bp        

>NTDB_id=785449 PSH92_RS24130 WP_122564964.1 5508676..5509083(-) (pilA) [Pseudomonas beijingensis strain FP2034]
ATGAATAAGCAAAAGGGTTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATCCTGGCGACGTTCGCGTT
GCCTCAATATTCCAGGTATCAAGCGCGGGCGAAGGCTACCGCTGGGTTGGGGGAAATCTCGGCGCTGAAGGTAGCGTACG
AGGATCAAATGAATCAGGGCGTTACTCCAACGTTGGCCCTCATGAACGCCCCGACCACAACCAGCAACTGCACAATTTCG
GTAACAGGTACGGCTACGACGGCAGGCTCTATCGTTTGTACCCTCCTGAACGCCCCTGCCTCTATAGCAGGTAGGACGAT
CACCCTCAGCCGCGATGTCAACGGCGCATGGACATGCGCATCCACCGCACCCAGGGAATTCCTGCCGACCTCTTGCCCTG
GCACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.175

100

0.489
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

100

0.444
  comP Acinetobacter baylyi ADP1

38.255

100

0.422
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.537

99.259

0.422
  pilA/pilA1 Eikenella corrodens VA1

36.735

100

0.4
  pilA Vibrio cholerae C6706

37.589

100

0.393
  pilA Vibrio cholerae strain A1552

37.589

100

0.393
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.589

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.417

100

0.378
  pilA Pseudomonas aeruginosa PAK

34.459

100

0.378
  pilA2 Legionella pneumophila str. Paris

34.722

100

0.37
  pilE Neisseria gonorrhoeae MS11

36.567

99.259

0.363