Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH92_RS24135 Genome accession   NZ_CP117451
Coordinates   5509308..5511008 (+) Length   566 a.a.
NCBI ID   WP_122564916.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5504308..5516008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH92_RS24115 (PSH92_24110) - 5504443..5507376 (+) 2934 WP_305468529.1 DUF748 domain-containing protein -
  PSH92_RS24120 (PSH92_24115) - 5507378..5507674 (+) 297 WP_305468531.1 DUF2845 domain-containing protein -
  PSH92_RS24125 (PSH92_24120) - 5508055..5508408 (-) 354 WP_025215523.1 BON domain-containing protein -
  PSH92_RS24130 (PSH92_24125) pilA 5508676..5509083 (-) 408 WP_122564964.1 pilin Machinery gene
  PSH92_RS24135 (PSH92_24130) pilB 5509308..5511008 (+) 1701 WP_122564916.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH92_RS24140 (PSH92_24135) pilC 5511011..5512228 (+) 1218 WP_122564917.1 type II secretion system F family protein Machinery gene
  PSH92_RS24145 (PSH92_24140) pilD 5512230..5513099 (+) 870 WP_305468535.1 A24 family peptidase Machinery gene
  PSH92_RS24150 (PSH92_24145) coaE 5513112..5513735 (+) 624 WP_122564919.1 dephospho-CoA kinase -
  PSH92_RS24155 (PSH92_24150) yacG 5513732..5513938 (+) 207 WP_018609255.1 DNA gyrase inhibitor YacG -
  PSH92_RS24160 (PSH92_24155) - 5513935..5514150 (-) 216 WP_053125334.1 hypothetical protein -
  PSH92_RS24165 (PSH92_24160) - 5514214..5514900 (-) 687 WP_305468537.1 energy-coupling factor ABC transporter permease -
  PSH92_RS24170 (PSH92_24165) - 5515060..5515686 (+) 627 WP_025215531.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62666.70 Da        Isoelectric Point: 5.4307

>NTDB_id=785450 PSH92_RS24135 WP_122564916.1 5509308..5511008(+) (pilB) [Pseudomonas beijingensis strain FP2034]
MNDIALSGLTKQLVLAELITEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKEAQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESTSTGLEGMGDADLDG
LDIESIDDTKQDSIGGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEVPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785450 PSH92_RS24135 WP_122564916.1 5509308..5511008(+) (pilB) [Pseudomonas beijingensis strain FP2034]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAACTGGTGCTGGCCGAACTGATCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCGCAACGCAGCCGCATCCCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTCCAGAGCCGTCAGGTCG
CGGAAATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAGCCCAGCCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCATGCCCTGCCGCTGTGGCGACGCGGCAACAAACTGTTCGTGGGCATCTCCGA
CCCGACTAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGATA
AACTCAGCGACGCCATCGAGAAGTTCTTCGAATCCACAAGTACGGGCCTGGAAGGCATGGGCGATGCCGACCTCGACGGC
CTGGACATCGAGTCGATCGACGACACCAAGCAGGATTCCATCGGCGGCCAGGACACCGACGACGCGCCCGTGGTGCGTTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAAAAAATCTACCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTGGCCCGGCCGCCGATCCAACTGGCGACCCGTATTGCCGCCCGCCTG
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGCCGATTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACGCTCTGGGGCGAAAAAGTGGTAATCCGGATCCTCGACCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTATGAACCGGATCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCACAAGGG
CTGATCCTGGTCACCGGACCGACCGGCTCGGGCAAGACCGTATCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCTTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCTTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAGGCCATCGAGGTTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGCTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGCTTCAATGACCTGCGCACATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.011

100

0.59
  pilB Acinetobacter baumannii D1279779

56.915

99.647

0.567
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.18

96.996

0.477