Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PSH84_RS24115 Genome accession   NZ_CP117425
Coordinates   5442304..5442711 (-) Length   135 a.a.
NCBI ID   WP_122564964.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP830     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5437304..5447711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH84_RS24100 (PSH84_24095) - 5438116..5441049 (+) 2934 WP_305481916.1 DUF748 domain-containing protein -
  PSH84_RS24105 (PSH84_24100) - 5441051..5441347 (+) 297 WP_305468531.1 DUF2845 domain-containing protein -
  PSH84_RS24110 (PSH84_24105) - 5441683..5442036 (-) 354 WP_025215523.1 BON domain-containing protein -
  PSH84_RS24115 (PSH84_24110) pilA 5442304..5442711 (-) 408 WP_122564964.1 pilin Machinery gene
  PSH84_RS24120 (PSH84_24115) pilB 5442936..5444636 (+) 1701 WP_305481917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH84_RS24125 (PSH84_24120) pilC 5444639..5445856 (+) 1218 WP_122564917.1 type II secretion system F family protein Machinery gene
  PSH84_RS24130 (PSH84_24125) pilD 5445858..5446727 (+) 870 WP_305468535.1 A24 family peptidase Machinery gene
  PSH84_RS24135 (PSH84_24130) coaE 5446740..5447363 (+) 624 WP_122564919.1 dephospho-CoA kinase -
  PSH84_RS24140 (PSH84_24135) yacG 5447360..5447566 (+) 207 WP_018609255.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14010.20 Da        Isoelectric Point: 8.4989

>NTDB_id=784785 PSH84_RS24115 WP_122564964.1 5442304..5442711(-) (pilA) [Pseudomonas beijingensis strain FP830]
MNKQKGFTLIELLIVVAIIGILATFALPQYSRYQARAKATAGLGEISALKVAYEDQMNQGVTPTLALMNAPTTTSNCTIS
VTGTATTAGSIVCTLLNAPASIAGRTITLSRDVNGAWTCASTAPREFLPTSCPGT

Nucleotide


Download         Length: 408 bp        

>NTDB_id=784785 PSH84_RS24115 WP_122564964.1 5442304..5442711(-) (pilA) [Pseudomonas beijingensis strain FP830]
ATGAATAAGCAAAAGGGTTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATCCTGGCGACGTTCGCGTT
GCCTCAATATTCCAGGTATCAAGCGCGGGCGAAGGCTACCGCTGGGCTGGGGGAAATCTCGGCGCTGAAGGTAGCGTACG
AGGATCAAATGAATCAGGGCGTTACTCCAACGTTGGCCCTCATGAACGCCCCGACCACAACCAGCAACTGCACAATTTCG
GTAACAGGTACGGCTACGACGGCAGGCTCTATCGTTTGTACCCTCCTGAACGCCCCTGCCTCTATAGCAGGTAGGACGAT
CACCCTCAGCCGCGATGTCAACGGCGCCTGGACATGCGCATCCACCGCACCCAGGGAATTCCTGCCGACCTCTTGCCCTG
GCACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.175

100

0.489
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

100

0.444
  comP Acinetobacter baylyi ADP1

38.255

100

0.422
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.537

99.259

0.422
  pilA/pilA1 Eikenella corrodens VA1

36.735

100

0.4
  pilA Vibrio cholerae C6706

37.589

100

0.393
  pilA Vibrio cholerae strain A1552

37.589

100

0.393
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.589

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.417

100

0.378
  pilA Pseudomonas aeruginosa PAK

34.459

100

0.378
  pilA2 Legionella pneumophila str. Paris

34.722

100

0.37
  pilE Neisseria gonorrhoeae MS11

36.567

99.259

0.363