Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PQE20_RS07950 Genome accession   NZ_CP117054
Coordinates   1681810..1682916 (-) Length   368 a.a.
NCBI ID   WP_017817653.1    Uniprot ID   -
Organism   Vibrio harveyi strain VH21FL     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1676810..1687916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQE20_RS07920 (PQE20_07920) - 1677395..1677892 (+) 498 WP_009697583.1 SprT family zinc-dependent metalloprotease -
  PQE20_RS07925 (PQE20_07925) endA 1678044..1678739 (+) 696 WP_050908042.1 deoxyribonuclease I -
  PQE20_RS07930 (PQE20_07930) rsmE 1678886..1679617 (+) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PQE20_RS07935 (PQE20_07935) gshB 1679632..1680582 (+) 951 WP_005449741.1 glutathione synthase -
  PQE20_RS07940 (PQE20_07940) - 1680712..1681275 (+) 564 WP_005449740.1 YqgE/AlgH family protein -
  PQE20_RS07945 (PQE20_07945) ruvX 1681325..1681750 (+) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  PQE20_RS07950 (PQE20_07950) pilU 1681810..1682916 (-) 1107 WP_017817653.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PQE20_RS07955 (PQE20_07955) pilT 1682948..1683988 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  PQE20_RS07960 (PQE20_07960) - 1684017..1684727 (+) 711 WP_005449733.1 YggS family pyridoxal phosphate-dependent enzyme -
  PQE20_RS07965 (PQE20_07965) proC 1684859..1685677 (+) 819 WP_050904301.1 pyrroline-5-carboxylate reductase -
  PQE20_RS07970 (PQE20_07970) - 1685731..1686288 (+) 558 WP_005425806.1 YggT family protein -
  PQE20_RS07975 (PQE20_07975) yggU 1686288..1686578 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  PQE20_RS07980 (PQE20_07980) - 1686646..1687077 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  PQE20_RS07985 (PQE20_07985) - 1687183..1687785 (+) 603 WP_272759815.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41277.40 Da        Isoelectric Point: 6.7730

>NTDB_id=782382 PQE20_RS07950 WP_017817653.1 1681810..1682916(-) (pilU) [Vibrio harveyi strain VH21FL]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=782382 PQE20_RS07950 WP_017817653.1 1681810..1682916(-) (pilU) [Vibrio harveyi strain VH21FL]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCAA
TGGACCCGGAGCGACGACAAGAGTTTCGCAAAAGCCGTGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAATTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACTATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37