Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PKO50_RS25925 Genome accession   NZ_CP116775
Coordinates   5684969..5685370 (-) Length   133 a.a.
NCBI ID   WP_275627638.1    Uniprot ID   -
Organism   Pseudomonas sp. 273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5672882..5687300 5684969..5685370 within 0


Gene organization within MGE regions


Location: 5672882..5687300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKO50_RS25850 - 5672882..5673557 (+) 676 Protein_5116 HipA domain-containing protein -
  PKO50_RS25855 - 5673551..5673766 (+) 216 Protein_5117 nicotinate-nucleotide diphosphorylase (carboxylating) -
  PKO50_RS25860 - 5673779..5674111 (-) 333 WP_109931574.1 DUF5615 family PIN-like protein -
  PKO50_RS25865 - 5674108..5674335 (-) 228 WP_109931572.1 DUF433 domain-containing protein -
  PKO50_RS25870 - 5674490..5674780 (-) 291 Protein_5120 type II toxin-antitoxin system HicB family antitoxin -
  PKO50_RS25875 - 5674777..5675850 (-) 1074 WP_275627630.1 diguanylate cyclase -
  PKO50_RS25885 - 5676270..5677574 (+) 1305 WP_089390525.1 nucleotide sugar dehydrogenase -
  PKO50_RS25890 - 5677585..5678277 (+) 693 WP_275627631.1 class I SAM-dependent methyltransferase -
  PKO50_RS25895 - 5678274..5678591 (+) 318 WP_275627632.1 hypothetical protein -
  PKO50_RS25900 - 5678615..5679283 (+) 669 WP_275627633.1 hypothetical protein -
  PKO50_RS25905 - 5680224..5681048 (-) 825 WP_275627634.1 hypothetical protein -
  PKO50_RS25910 - 5681045..5682133 (-) 1089 WP_275627635.1 UDP-N-acetylglucosamine 2-epimerase -
  PKO50_RS25915 - 5682135..5683010 (-) 876 WP_275627636.1 glycosyltransferase family 2 protein -
  PKO50_RS25920 - 5683011..5684909 (-) 1899 WP_275627637.1 hypothetical protein -
  PKO50_RS25925 pilA/pilAI 5684969..5685370 (-) 402 WP_275627638.1 pilin Machinery gene
  PKO50_RS25930 pilB 5685597..5687300 (+) 1704 WP_275627639.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13741.69 Da        Isoelectric Point: 5.7985

>NTDB_id=780224 PKO50_RS25925 WP_275627638.1 5684969..5685370(-) (pilA/pilAI) [Pseudomonas sp. 273]
MKVQKGFTLIELMIVVAIIGILAAIAIPAYSKYQAKAKVAAGLAEISAGKTAFETRLNDGDTVSTVAQIGLTASTSNCNI
SVTGNSIECELVHAPSQVEGGTITWTRTASTGSWACATDGSKITDEYAPKGCQ

Nucleotide


Download         Length: 402 bp        

>NTDB_id=780224 PKO50_RS25925 WP_275627638.1 5684969..5685370(-) (pilA/pilAI) [Pseudomonas sp. 273]
ATGAAAGTTCAAAAAGGTTTCACCCTGATCGAGTTGATGATCGTTGTTGCGATTATTGGTATTCTGGCTGCTATTGCTAT
TCCTGCTTACAGTAAGTACCAAGCTAAAGCTAAGGTGGCTGCGGGCTTGGCCGAGATTTCTGCCGGTAAGACTGCATTCG
AAACCCGACTGAATGATGGCGACACCGTCAGTACTGTCGCTCAGATTGGCCTGACTGCTTCTACCTCTAACTGCAACATT
AGTGTTACTGGTAACTCTATCGAGTGTGAGCTGGTTCATGCTCCTTCTCAAGTTGAAGGCGGGACTATCACTTGGACTCG
CACTGCATCCACTGGCTCTTGGGCCTGTGCTACGGATGGTTCCAAAATTACTGATGAATATGCGCCGAAAGGCTGCCAGT
AA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.482

100

0.496
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.118

100

0.451
  pilA Acinetobacter baumannii strain A118

42.553

100

0.451
  pilA Vibrio cholerae strain A1552

40.268

100

0.451
  pilA Vibrio cholerae C6706

40.268

100

0.451
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.268

100

0.451
  comP Acinetobacter baylyi ADP1

39.597

100

0.444
  pilA Pseudomonas aeruginosa PAK

38.411

100

0.436
  pilA2 Legionella pneumophila strain ERS1305867

39.416

100

0.406
  pilA2 Legionella pneumophila str. Paris

39.416

100

0.406
  pilE Neisseria gonorrhoeae MS11

33.121

100

0.391
  pilA Vibrio parahaemolyticus RIMD 2210633

42.149

90.977

0.383
  pilA Ralstonia pseudosolanacearum GMI1000

37.121

99.248

0.368