Detailed information NTG0780225

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PKO50_RS25930
• Genome accession: NZ_CP116775
• Coordinates: 5685597..5687300 (+)
• Length: 567 a.a.
• NCBI ID: WP_275627639.1
• Uniprot ID: -
• Organism: Pseudomonas sp. 273
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5672882..5687300	5685597..5687300	within	0

Gene organization within MGE regions

Location: 5672882..5687300

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKO50_RS25850	-	5672882..5673557 (+)	676	Protein_5116	HipA domain-containing protein	-
PKO50_RS25855	-	5673551..5673766 (+)	216	Protein_5117	nicotinate-nucleotide diphosphorylase (carboxylating)	-
PKO50_RS25860	-	5673779..5674111 (-)	333	WP_109931574.1	DUF5615 family PIN-like protein	-
PKO50_RS25865	-	5674108..5674335 (-)	228	WP_109931572.1	DUF433 domain-containing protein	-
PKO50_RS25870	-	5674490..5674780 (-)	291	Protein_5120	type II toxin-antitoxin system HicB family antitoxin	-
PKO50_RS25875	-	5674777..5675850 (-)	1074	WP_275627630.1	diguanylate cyclase	-
PKO50_RS25885	-	5676270..5677574 (+)	1305	WP_089390525.1	nucleotide sugar dehydrogenase	-
PKO50_RS25890	-	5677585..5678277 (+)	693	WP_275627631.1	class I SAM-dependent methyltransferase	-
PKO50_RS25895	-	5678274..5678591 (+)	318	WP_275627632.1	hypothetical protein	-
PKO50_RS25900	-	5678615..5679283 (+)	669	WP_275627633.1	hypothetical protein	-
PKO50_RS25905	-	5680224..5681048 (-)	825	WP_275627634.1	hypothetical protein	-
PKO50_RS25910	-	5681045..5682133 (-)	1089	WP_275627635.1	UDP-N-acetylglucosamine 2-epimerase	-
PKO50_RS25915	-	5682135..5683010 (-)	876	WP_275627636.1	glycosyltransferase family 2 protein	-
PKO50_RS25920	-	5683011..5684909 (-)	1899	WP_275627637.1	hypothetical protein	-
PKO50_RS25925	pilA/pilAI	5684969..5685370 (-)	402	WP_275627638.1	pilin	Machinery gene
PKO50_RS25930	pilB	5685597..5687300 (+)	1704	WP_275627639.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62742.83 Da
• Isoelectric Point: 5.4667

>NTDB_id=780225 PKO50_RS25930 WP_275627639.1 5685597..5687300(+) (pilB) [Pseudomonas sp. 273]
MNDQVVLSGLARQLVIAELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLGAIDKDSLPKE
LVSEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDTIEKLFESATGGLDDLGDVDLE
GLDIESGNTERKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEEQKELYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCTHCKKEHDIPRETLLHEGFP
EERIGSFKLYSPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRISGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=780225 PKO50_RS25930 WP_275627639.1 5685597..5687300(+) (pilB) [Pseudomonas sp. 273]
ATGAACGATCAAGTCGTGCTCTCCGGCCTCGCCCGCCAACTAGTGATCGCCGAGCTTCTTGACGAGAAGAGCGCCCAGCA
AGCGCAACAACAGGCTCAGCGCAACAAGCTGTCGTTGGTCACCTATCTTGTGCAGAACAAATTGGTGAAAAGCCGCCCAT
TGACGGAGCTAGCGGCTGACCAGTTCGGTGTTGCCTTCTGCGACCTGGGAGCCATCGACAAGGACAGTCTTCCCAAGGAG
CTAGTCAGCGAAAAGCTGGTGCGCCAACACCGAGTCATCCCACTCTGGCGCCGAGGCAATAAGCTCTTTGTGGGTATCTC
CGACCCTACCAACCACCAGGCCATTACCGACGTTCAGTTCAGTACCGGCCTGACCACTGAGGCCATCCTGGTTGAGGACG
ACAAGCTCGGAGATACCATCGAAAAACTCTTCGAGAGCGCTACCGGCGGGCTGGATGACCTAGGCGACGTCGATCTAGAA
GGCCTGGATATCGAAAGTGGAAACACCGAGCGCAAAGAAGAGTCCATCGGAGGGGACGCCGACGATGCCCCAGTGGTCCG
CTTCGTCAACAAAATGCTGTTGGACGCCATCAAGGGGGGCTCCTCCGACTTGCACTTCGAGCCCTACGAAAAAGTCTACC
GCGTGCGCTTCCGTACCGACGGTATGCTACACGAGGTAGCCAAACCCCCCATTCAACTGGCAAGTCGCATCTCCGCACGC
CTGAAGGTAATGGCCGGCTTGGATATTTCCGAGCGGCGCAAGCCCCAGGACGGTCGCATCAAGATGCGTGTATCCAAGAG
CAAATCCATCGACTTCCGCGTTAACACCCTGCCTACGCTGTGGGGCGAGAAAATCGTGATGCGGATTCTCGACTCGGCCA
GCGCGCAGATGGGCATCGATGCGCTGGGTTATGAGGAGGAGCAGAAAGAACTTTACCTGGAAGCCCTCAAGCAGCCCCAG
GGCATGATCCTAGTGACAGGCCCCACCGGCTCGGGCAAGACGGTATCCCTCTATACGGGCCTGAACATCCTCAATACCCC
AGACATCAATATCTCCACCGCAGAGGACCCGGTGGAGATCAACCTGGAGGGTATCAACCAGGTCAACGTCAACCCTCGCC
AGGGCATGGATTTCGCCCAGGCCCTGCGCGCCTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGAC
CTTGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCATATGGTGATGTCCACCCTGCACACCAACAGTGCCGC
AGAAACCCTCACGCGCCTGCTGAACATGGGCGTGGCCTCCTTCAACCTGGCTACATCGACCAACCTGATCATCGCCCAAC
GCCTGGCGCGCAAGCTCTGCACCCACTGCAAGAAGGAGCACGATATCCCACGCGAAACCCTGCTTCATGAGGGCTTCCCC
GAGGAGCGGATCGGCAGCTTCAAGCTGTACTCCCCCGTGGGGTGCGAAAATTGCAAGAATGGCTACAAGGGTCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCTGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTACGCATCTCGGGCCTGCTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.23	99.647	0.58
pilB	Acinetobacter baylyi ADP1	57.979	99.471	0.577
pilB	Legionella pneumophila strain ERS1305867	55.026	100	0.55
pilB	Vibrio campbellii strain DS40M4	49.912	99.647	0.497
pilB	Vibrio cholerae strain A1552	50.089	99.295	0.497
pilF	Neisseria gonorrhoeae MS11	49.735	100	0.497
pilB	Vibrio parahaemolyticus RIMD 2210633	50.923	95.591	0.487
pilF	Thermus thermophilus HB27	38.489	100	0.386
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.076	93.298	0.374