Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PMC74_RS24860 Genome accession   NZ_CP116669
Coordinates   5553365..5554363 (-) Length   332 a.a.
NCBI ID   WP_047582189.1    Uniprot ID   A0A6L6TDV4
Organism   Pseudomonas capeferrum strain TDA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5548365..5559363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMC74_RS24840 (PMC74_24840) argS 5549531..5551267 (+) 1737 WP_047582193.1 arginine--tRNA ligase -
  PMC74_RS24845 (PMC74_24845) - 5551269..5551967 (+) 699 WP_047582191.1 SPOR domain-containing protein -
  PMC74_RS24850 (PMC74_24850) - 5552027..5552446 (-) 420 WP_033702015.1 NINE protein -
  PMC74_RS24855 (PMC74_24855) - 5552614..5553237 (+) 624 WP_033702017.1 C40 family peptidase -
  PMC74_RS24860 (PMC74_24860) pilT 5553365..5554363 (-) 999 WP_047582189.1 type IV pilus twitching motility protein PilT Machinery gene
  PMC74_RS24865 (PMC74_24865) - 5554429..5555115 (+) 687 WP_033702019.1 YggS family pyridoxal phosphate-dependent enzyme -
  PMC74_RS24870 (PMC74_24870) proC 5555154..5555972 (+) 819 WP_033702020.1 pyrroline-5-carboxylate reductase -
  PMC74_RS24875 (PMC74_24875) - 5555983..5556573 (+) 591 WP_033702021.1 YggT family protein -
  PMC74_RS24880 (PMC74_24880) metX 5556674..5557813 (+) 1140 WP_033702022.1 homoserine O-succinyltransferase MetX -
  PMC74_RS24885 (PMC74_24885) metW 5557821..5558441 (+) 621 WP_033702023.1 methionine biosynthesis protein MetW -
  PMC74_RS24890 (PMC74_24890) - 5558464..5558898 (+) 435 WP_033702024.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35737.36 Da        Isoelectric Point: 6.3926

>NTDB_id=778980 PMC74_RS24860 WP_047582189.1 5553365..5554363(-) (pilT) [Pseudomonas capeferrum strain TDA1]
MDVTDLLARAVEAGASDLHLAAGQVPMLRLDGELQRMDAPPLLSAALSDAMTPLLADEQCQQWAQGDELDVALQLPALGR
FRLNLFRQLNGLAATFRVIPKRIATLDELDLGEVFLAVAQCTDGLVLVGGPTGSGKSSTLAALLDQLNRERPVHIITLED
PIEVIHSSQRSLVNQREIGRHCGGFAQGLRSALRQDPDVIVIGELRDLQTIRLALRAAETGHLVLATVHTRSAINSIDRL
VEVFAAEEKPLVRAMLAESLRVVVAQVLVKRVGGGRVAAREVLVANTAVRNLIREGRLAQLQSVMQSGAAAGMRTMEAAL
RELHAKGLTETL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=778980 PMC74_RS24860 WP_047582189.1 5553365..5554363(-) (pilT) [Pseudomonas capeferrum strain TDA1]
ATGGATGTGACCGACCTGTTGGCCCGTGCCGTGGAAGCGGGGGCTTCGGACCTGCACCTGGCTGCAGGCCAGGTACCGAT
GCTGCGCCTTGATGGCGAGCTGCAACGCATGGACGCGCCGCCACTGCTGTCGGCGGCCCTGAGCGATGCGATGACACCCT
TGCTGGCGGATGAGCAGTGCCAGCAATGGGCACAGGGTGATGAGCTGGACGTCGCGTTGCAACTGCCAGCGCTGGGCCGC
TTTCGCCTGAACCTGTTTCGCCAGTTGAACGGCCTGGCGGCAACCTTTCGCGTGATCCCGAAACGCATAGCGACGCTCGA
TGAACTCGACCTGGGCGAAGTGTTTCTAGCTGTTGCGCAGTGTACCGATGGCCTGGTTCTGGTGGGCGGGCCAACCGGCA
GCGGCAAGTCCAGCACCTTGGCGGCGCTGCTCGATCAACTCAACCGCGAACGGCCTGTGCATATCATCACGCTGGAAGAC
CCTATCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAATCAGCGCGAAATTGGCCGCCATTGTGGCGGGTTCGCTCA
GGGGCTGCGCAGTGCGCTGCGTCAGGACCCGGATGTGATCGTGATTGGTGAGTTGCGTGATCTGCAGACCATACGCCTGG
CGTTGCGGGCGGCGGAGACGGGGCACCTGGTGCTGGCGACGGTGCACACCCGTTCGGCCATCAATAGCATCGACCGATTG
GTCGAGGTCTTTGCTGCCGAGGAAAAGCCACTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGGGTGGTGGTGGCGCAGGT
GCTGGTCAAGCGCGTGGGCGGCGGCCGTGTTGCGGCGCGGGAAGTGCTGGTGGCCAACACGGCGGTGCGTAACCTGATCC
GTGAGGGGCGGCTGGCGCAGTTGCAGTCGGTGATGCAGTCGGGAGCCGCGGCGGGCATGCGGACGATGGAGGCGGCGTTG
CGTGAGCTGCACGCTAAAGGATTGACCGAAACCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L6TDV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.744

98.795

0.521
  pilT Acinetobacter baylyi ADP1

51.829

98.795

0.512
  pilT Pseudomonas stutzeri DSM 10701

51.524

98.795

0.509
  pilT Acinetobacter baumannii D1279779

51.22

98.795

0.506
  pilT Acinetobacter baumannii strain A118

51.22

98.795

0.506
  pilT Acinetobacter nosocomialis M2

50.915

98.795

0.503
  pilT Vibrio cholerae strain A1552

50.606

99.398

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

99.398

0.503
  pilT Neisseria meningitidis 8013

49.544

99.096

0.491
  pilT Legionella pneumophila strain Lp02

49.695

98.795

0.491
  pilT Legionella pneumophila strain ERS1305867

49.695

98.795

0.491
  pilT Neisseria gonorrhoeae MS11

49.24

99.096

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.289

99.096

0.449
  pilU Pseudomonas stutzeri DSM 10701

36.585

98.795

0.361