Detailed information NTG0774564

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PG909_RS10155
• Genome accession: NZ_CP115946
• Coordinates: 2347279..2348418 (-)
• Length: 379 a.a.
• NCBI ID: WP_003263110.1
• Uniprot ID: -
• Organism: Ralstonia solanacearum MolK2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2342279..2353418

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PG909_RS10130	-	2343993..2344529 (-)	537	WP_003263105.1	PilX N-terminal domain-containing pilus assembly protein	-
PG909_RS10135	-	2344533..2345567 (-)	1035	WP_003273412.1	PilW family protein	-
PG909_RS10140	pilV	2345564..2346154 (-)	591	WP_003263107.1	type IV pilus modification protein PilV	-
PG909_RS10145	-	2346151..2346639 (-)	489	WP_042550590.1	GspH/FimT family pseudopilin	-
PG909_RS10150	-	2346643..2347083 (-)	441	WP_003263109.1	type IV pilin protein	-
PG909_RS10155	pilU	2347279..2348418 (-)	1140	WP_003263110.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PG909_RS10160	pilT	2348468..2349511 (-)	1044	WP_003263111.1	type IV pilus twitching motility protein PilT	Machinery gene
PG909_RS10165	-	2349633..2350355 (+)	723	WP_003273408.1	YggS family pyridoxal phosphate-dependent enzyme	-
PG909_RS10170	proC	2350390..2351214 (+)	825	WP_003273407.1	pyrroline-5-carboxylate reductase	-
PG909_RS10175	-	2351223..2351501 (+)	279	WP_003273406.1	hypothetical protein	-
PG909_RS10180	ubiA	2351498..2352373 (-)	876	WP_003273404.1	4-hydroxybenzoate octaprenyltransferase	-
PG909_RS10185	-	2352447..2352947 (-)	501	WP_003271865.1	Dps family protein	-
PG909_RS10190	-	2353106..2353348 (-)	243	WP_003263116.1	hypothetical protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 42207.65 Da
• Isoelectric Point: 6.9314

>NTDB_id=774564 PG909_RS10155 WP_003263110.1 2347279..2348418(-) (pilU) [Ralstonia solanacearum MolK2]
MLDRDAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREYEDSFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTINTKIPTLTELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIVNFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVTFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHGKRGGPADLTAGTEHLNVM

Nucleotide

• Length: 1140 bp

>NTDB_id=774564 PG909_RS10155 WP_003263110.1 2347279..2348418(-) (pilU) [Ralstonia solanacearum MolK2]
ATGCTGGACCGCGACGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACCTGTT
CATCACCGCCGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAACTGCGCGAGTACGAAGACTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACCAAGATCCCGACGCTCACCGAACTGGATCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTCGTGGTGGGGGCGACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATACGTGCATGCGCACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
CGTGGATACGGAGTCATGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATTCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCGTCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCGATGATTGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGCCGTGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCGGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAATCCCGCGAGCAG
GGGATGGTCACGTTCGACCAGGCACTGTTCGAGCTTTATGAAGCGGACAAGATCACCTATGAAGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCACGGCAAGCGCGGCGGGCCGGCGGATCTGACGGCCGGTACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.756	93.14	0.575
pilU	Acinetobacter baylyi ADP1	54.875	94.723	0.52
pilU	Vibrio cholerae strain A1552	55.367	93.404	0.517
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.917	88.918	0.391
pilT	Legionella pneumophila strain Lp02	43.844	87.863	0.385
pilT	Legionella pneumophila strain ERS1305867	43.844	87.863	0.385
pilT	Pseudomonas aeruginosa PAK	43.027	88.918	0.383
pilT	Vibrio cholerae strain A1552	45.741	83.641	0.383
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	45.741	83.641	0.383
pilT	Acinetobacter baylyi ADP1	44.72	84.96	0.38
pilT	Pseudomonas stutzeri DSM 10701	41.84	88.918	0.372
pilT	Acinetobacter nosocomialis M2	43.887	84.169	0.369
pilT	Acinetobacter baumannii D1279779	43.887	84.169	0.369
pilT	Acinetobacter baumannii strain A118	43.887	84.169	0.369