Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PGN41_RS05295 Genome accession   NZ_CP115820
Coordinates   1118567..1118995 (-) Length   142 a.a.
NCBI ID   WP_271105071.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain Q4-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1113567..1123995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGN41_RS05265 - 1113640..1113855 (+) 216 WP_213628504.1 hypothetical protein -
  PGN41_RS05270 yacG 1113859..1114059 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  PGN41_RS05275 coaE 1114056..1114667 (-) 612 WP_271105067.1 dephospho-CoA kinase -
  PGN41_RS05280 pilD 1114667..1115536 (-) 870 WP_271105068.1 prepilin peptidase Machinery gene
  PGN41_RS05285 pilC 1115537..1116757 (-) 1221 WP_271105069.1 type II secretion system F family protein Machinery gene
  PGN41_RS05290 pilB 1116761..1118464 (-) 1704 WP_271105070.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGN41_RS05295 pilA 1118567..1118995 (-) 429 WP_271105071.1 pilin Machinery gene
  PGN41_RS05300 - 1119106..1120851 (+) 1746 WP_271105072.1 PglL family O-oligosaccharyltransferase -
  PGN41_RS05310 nadC 1121033..1121881 (-) 849 WP_271105073.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14909.99 Da        Isoelectric Point: 9.1475

>NTDB_id=773853 PGN41_RS05295 WP_271105071.1 1118567..1118995(-) (pilA) [Pseudomonas tohonis strain Q4-3]
MKTQKGFTLIELMIVVAIIGILAAIAIPAYRNYIARSESNTGLQAIAPLKTAVEDGYARGLLGNSVASLSVLGTTATASP
LGTISAVFADDGSGTLTFTFDRQSSPTLKTAGQNTHVLQRRTDGTWSCTSTVPTDFRPRGCN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=773853 PGN41_RS05295 WP_271105071.1 1118567..1118995(-) (pilA) [Pseudomonas tohonis strain Q4-3]
ATGAAAACCCAAAAAGGCTTCACCCTGATCGAACTGATGATTGTTGTGGCAATCATCGGCATTCTGGCAGCTATCGCCAT
CCCGGCCTACCGTAACTATATCGCTCGTTCCGAGTCTAACACCGGCCTTCAAGCGATCGCACCGCTGAAAACCGCTGTAG
AAGACGGCTATGCGCGCGGCCTGCTGGGCAACTCCGTCGCCAGCCTGAGCGTACTGGGCACCACCGCCACCGCCAGCCCG
CTTGGCACCATTTCCGCTGTCTTCGCGGACGATGGCTCCGGCACCCTGACCTTCACCTTCGACCGTCAGTCCAGCCCCAC
GCTGAAGACAGCTGGCCAGAACACCCACGTTCTGCAGCGCCGTACCGATGGAACTTGGTCCTGCACCTCCACCGTGCCCA
CCGACTTCCGCCCCCGCGGCTGCAACTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.358

100

0.493
  pilA Vibrio parahaemolyticus RIMD 2210633

45.39

99.296

0.451
  pilA Acinetobacter baumannii strain A118

45.39

99.296

0.451
  pilA Vibrio cholerae C6706

40.94

100

0.43
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.94

100

0.43
  pilA Vibrio cholerae strain A1552

40.94

100

0.43
  comP Acinetobacter baylyi ADP1

39.333

100

0.415
  pilA2 Legionella pneumophila str. Paris

40.278

100

0.408
  pilA2 Legionella pneumophila strain ERS1305867

39.286

98.592

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.146

96.479

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.241

100

0.38