NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	PGN41_RS05280	Genome accession	NZ_CP115820
Coordinates	1114667..1115536 (-)	Length	289 a.a.
NCBI ID	WP_271105068.1	Uniprot ID	-
Organism	Pseudomonas tohonis strain Q4-3
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1109667..1120536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PGN41_RS05240	-	1110961..1111134 (-)	174	WP_173173318.1	DUF3094 domain-containing protein	-
PGN41_RS05245	-	1111221..1111847 (-)	627	WP_173173316.1	DUF1780 domain-containing protein	-
PGN41_RS05250	-	1111960..1112385 (+)	426	WP_173173313.1	GNAT family N-acetyltransferase	-
PGN41_RS05255	-	1112382..1112849 (+)	468	WP_271105066.1	FAD/FMN-containing dehydrogenase	-
PGN41_RS05260	-	1112846..1113532 (+)	687	WP_173173311.1	energy-coupling factor ABC transporter permease	-
PGN41_RS05265	-	1113640..1113855 (+)	216	WP_213628504.1	hypothetical protein	-
PGN41_RS05270	yacG	1113859..1114059 (-)	201	WP_111263622.1	DNA gyrase inhibitor YacG	-
PGN41_RS05275	coaE	1114056..1114667 (-)	612	WP_271105067.1	dephospho-CoA kinase	-
PGN41_RS05280	pilD	1114667..1115536 (-)	870	WP_271105068.1	prepilin peptidase	Machinery gene
PGN41_RS05285	pilC	1115537..1116757 (-)	1221	WP_271105069.1	type II secretion system F family protein	Machinery gene
PGN41_RS05290	pilB	1116761..1118464 (-)	1704	WP_271105070.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PGN41_RS05295	pilA	1118567..1118995 (-)	429	WP_271105071.1	pilin	Machinery gene

Sequence

Protein

Download Length: 289 a.a. Molecular weight: 31848.84 Da Isoelectric Point: 6.9296

                                >NTDB_id=773850 PGN41_RS05280 WP_271105068.1 1114667..1115536(-) (pilD) [Pseudomonas tohonis strain Q4-3]

MTVIDFLAGNLPAFVFCIFLLGLLVGSFLNVVIYRLPVMMFRDWKAQAREALELPAEPKGETFNLILPNSRCPHCQHEIQ

AWENIPVVSWLALGGKCSSCKAPIKKRYPLMELACGLLSAYVAWHFGFTWQAGAFLVLTWGLLAMSMIDVDHQLLPDSLV

LPLLWLGLILNNQGMFATPSDALWGAVGGYLSLWSVYWVFKLVTGKEGMGYGDFKLLAMIGAWGGWQVLPLTILLSSLVG

AILGIIILKLRDANSSTPIPFGPYLAIAGWIALLWGDQITGTYLHFAGF

Nucleotide

Download Length: 870 bp

                                >NTDB_id=773850 PGN41_RS05280 WP_271105068.1 1114667..1115536(-) (pilD) [Pseudomonas tohonis strain Q4-3]

GTGACAGTCATCGACTTCCTGGCCGGCAACTTGCCGGCCTTTGTTTTCTGCATCTTCCTCCTAGGCCTTCTCGTCGGCAG

CTTCCTCAATGTCGTCATCTACCGCCTGCCGGTGATGATGTTCCGTGACTGGAAGGCGCAGGCACGGGAGGCGTTGGAGT

TGCCGGCGGAGCCCAAAGGCGAGACCTTCAACCTGATCCTGCCGAACTCGCGCTGCCCGCATTGCCAGCATGAGATCCAG

GCCTGGGAAAACATCCCGGTGGTCAGTTGGCTGGCCCTGGGTGGCAAGTGCTCGTCGTGCAAGGCGCCGATCAAGAAGCG

CTACCCGCTGATGGAGCTGGCGTGCGGCCTGTTGTCGGCCTATGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCCGGGG

CTTTCCTGGTGCTGACCTGGGGCCTGCTGGCGATGAGCATGATCGACGTCGACCACCAGCTGCTGCCAGACTCCCTGGTG

CTGCCGCTGCTATGGCTCGGGCTGATCCTCAACAACCAGGGCATGTTCGCCACGCCCAGCGATGCGCTCTGGGGCGCGGT

GGGCGGCTATCTCAGCCTGTGGTCGGTCTACTGGGTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTACGGCGACT

TCAAGCTGCTGGCGATGATCGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATCCTGCTGTCGTCCCTGGTGGGG

GCGATCCTGGGAATCATCATCCTCAAGCTGCGCGACGCGAACAGCAGCACGCCCATCCCCTTCGGCCCCTACTTGGCCAT

TGCCGGCTGGATTGCATTGCTCTGGGGTGATCAAATAACCGGGACCTATCTCCACTTCGCCGGTTTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter baumannii D1279779	52.817	98.27	0.519
pilD	Vibrio cholerae strain A1552	56.015	92.042	0.516
pilD	Acinetobacter nosocomialis M2	52.113	98.27	0.512
pilD	Vibrio campbellii strain DS40M4	52.747	94.464	0.498
pilD	Neisseria gonorrhoeae MS11	50.192	90.311	0.453

Detailed information NTG0773850