Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PGN41_RS05290 Genome accession   NZ_CP115820
Coordinates   1116761..1118464 (-) Length   567 a.a.
NCBI ID   WP_271105070.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain Q4-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1111761..1123464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGN41_RS05250 - 1111960..1112385 (+) 426 WP_173173313.1 GNAT family N-acetyltransferase -
  PGN41_RS05255 - 1112382..1112849 (+) 468 WP_271105066.1 FAD/FMN-containing dehydrogenase -
  PGN41_RS05260 - 1112846..1113532 (+) 687 WP_173173311.1 energy-coupling factor ABC transporter permease -
  PGN41_RS05265 - 1113640..1113855 (+) 216 WP_213628504.1 hypothetical protein -
  PGN41_RS05270 yacG 1113859..1114059 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  PGN41_RS05275 coaE 1114056..1114667 (-) 612 WP_271105067.1 dephospho-CoA kinase -
  PGN41_RS05280 pilD 1114667..1115536 (-) 870 WP_271105068.1 prepilin peptidase Machinery gene
  PGN41_RS05285 pilC 1115537..1116757 (-) 1221 WP_271105069.1 type II secretion system F family protein Machinery gene
  PGN41_RS05290 pilB 1116761..1118464 (-) 1704 WP_271105070.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGN41_RS05295 pilA 1118567..1118995 (-) 429 WP_271105071.1 pilin Machinery gene
  PGN41_RS05300 - 1119106..1120851 (+) 1746 WP_271105072.1 PglL family O-oligosaccharyltransferase -
  PGN41_RS05310 nadC 1121033..1121881 (-) 849 WP_271105073.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62480.62 Da        Isoelectric Point: 5.6294

>NTDB_id=773852 PGN41_RS05290 WP_271105070.1 1116761..1118464(-) (pilB) [Pseudomonas tohonis strain Q4-3]
MSDSISLTGLARQIVIAELLDEKSAQQAQLQALRNKMSLVTYIVQNKLVKSRPLAEIAAEQFGVAFFDLSAIDKDSQPKD
LISEKLIRQHRVIPLWRRGNKLFIGVSDPTNHQAITDVQFSTGLTTEAILVEDDKIGEAIERFFESSNSGMDDLGDVDLD
GVDIESVDEDKDDKGNGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNSLPTLWGEKIVMRILDPSSAQMGIDALGYEEVQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSSCKKEVEVPRETLLKEGFP
ESKIGTFKIYGPVGCEHCKGGYKGRVGIYEVVKNTTALQRIIMEEGNSIDIAAQMRKDGFNDLRASGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=773852 PGN41_RS05290 WP_271105070.1 1116761..1118464(-) (pilB) [Pseudomonas tohonis strain Q4-3]
ATGAGCGACTCAATTTCTCTTACAGGTCTGGCACGCCAGATCGTCATTGCGGAACTGCTTGACGAGAAAAGCGCTCAGCA
GGCGCAGCTACAGGCACTGCGAAACAAAATGTCGCTGGTGACCTATATAGTTCAGAACAAGCTTGTAAAAAGCCGTCCGC
TCGCGGAAATAGCCGCAGAGCAGTTTGGGGTGGCATTCTTCGACCTCTCCGCTATTGACAAAGATTCTCAACCCAAAGAT
CTCATCAGTGAAAAGCTTATTCGCCAGCACCGAGTCATTCCGCTCTGGAGAAGAGGCAACAAACTGTTCATTGGCGTATC
AGACCCAACCAATCACCAAGCCATCACAGACGTCCAATTCAGCACCGGTCTAACGACAGAGGCGATTTTGGTGGAGGACG
ACAAAATTGGCGAAGCGATAGAACGCTTCTTCGAGTCCTCTAACAGTGGCATGGACGATTTGGGTGATGTCGACCTAGAC
GGTGTCGACATCGAAAGCGTCGATGAGGACAAAGACGATAAAGGCAATGGTGGAGACGCAGACGACGCCCCAGTGGTTCG
CTTCGTCAACAAAATGCTGCTGGACGCGATCAAAGGAGGCTCTTCGGACCTGCATTTTGAGCCCTACGAAAAAAGCTACC
GCGTCCGCCTTCGCACCGACGGTATTCTCCACGAAGTGGCACGTCCTCCCATACAACTGGCCAGCAGGATATCTGCTCGC
TTAAAGGTCATGGCAGCACTTGACATCTCCGAGCGCCGCAAACCGCAGGACGGTCGGATAAAGATGAAAATCTCCAAGAC
CAAGGCCATCGACTTCCGCGTCAATAGCCTCCCGACACTCTGGGGCGAGAAGATCGTAATGCGGATTCTCGACCCTTCCA
GTGCTCAGATGGGTATCGATGCCCTCGGGTACGAGGAAGTGCAAAAAGAGCTCTATCTGAGCGCCCTGAAACAACCTCAA
GGCATGATCCTGGTGACAGGCCCAACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGCCTCAACATTCTCAATACGGT
CGATATCAACATTTCCACGGCAGAAGATCCCGTAGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCACGTC
AAGGCATGGACTTCGCCCAGGCCCTTCGCGCCTTCCTGCGCCAGGACCCTGATGTGATCATGGTCGGCGAGATTCGTGAC
CTCGAGACTGCCGAGATCGCCATTAAGGCGGCACAAACAGGTCACATGGTGATGTCCACACTGCACACCAACAGCGCAGC
GGAAACCCTGACGCGTCTTCGCAACATGGGCGTCCCGGCCTTCAACATCGCGACATCTGTCAACCTCATCATTGCGCAAC
GCCTGGCCAGAAAGCTTTGCAGCAGTTGCAAGAAGGAGGTTGAGGTGCCTCGTGAAACCCTGCTCAAGGAAGGTTTCCCT
GAGAGCAAGATCGGCACTTTCAAGATTTATGGCCCTGTGGGTTGCGAACACTGCAAGGGCGGCTACAAGGGCAGAGTCGG
TATTTATGAAGTGGTTAAAAACACGACCGCGCTACAACGCATTATCATGGAGGAAGGCAACTCTATTGATATCGCCGCGC
AAATGCGTAAAGACGGCTTCAATGACCTGCGCGCCTCGGGCCTGCTGAAAGCCATGCAAGGCGTAACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.019

100

0.594
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.552
  pilB Vibrio cholerae strain A1552

50.179

98.765

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.498

94.18

0.485
  pilB Vibrio campbellii strain DS40M4

50.56

94.533

0.478
  pilF Neisseria gonorrhoeae MS11

47.957

99.295

0.476
  pilF Thermus thermophilus HB27

37.279

99.824

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.298

0.369