Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7K64_RS01685 Genome accession   NZ_AP022227
Coordinates   359805..360803 (+) Length   332 a.a.
NCBI ID   WP_182816986.1    Uniprot ID   -
Organism   Pseudomonas putida strain WP8-W18-CRE-01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354805..365803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K64_RS01655 (WP8W18C01_03230) - 355276..355710 (-) 435 WP_043208621.1 DUF4426 domain-containing protein -
  H7K64_RS01660 (WP8W18C01_03240) metW 355742..356362 (-) 621 WP_043208620.1 methionine biosynthesis protein MetW -
  H7K64_RS01665 (WP8W18C01_03250) metX 356370..357509 (-) 1140 WP_070571721.1 homoserine O-succinyltransferase MetX -
  H7K64_RS01670 (WP8W18C01_03260) - 357597..358184 (-) 588 WP_043208615.1 YggT family protein -
  H7K64_RS01675 (WP8W18C01_03270) proC 358195..359013 (-) 819 WP_043208613.1 pyrroline-5-carboxylate reductase -
  H7K64_RS01680 (WP8W18C01_03280) - 359052..359738 (-) 687 WP_182816983.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7K64_RS01685 (WP8W18C01_03290) pilT 359805..360803 (+) 999 WP_182816986.1 type IV pilus twitching motility protein PilT Machinery gene
  H7K64_RS01690 (WP8W18C01_03300) - 360967..361596 (-) 630 WP_043208607.1 C40 family peptidase -
  H7K64_RS01695 (WP8W18C01_03310) - 361764..362180 (+) 417 WP_043208605.1 NINE protein -
  H7K64_RS01700 (WP8W18C01_03320) - 362224..362931 (-) 708 WP_043208603.1 SPOR domain-containing protein -
  H7K64_RS01705 (WP8W18C01_03330) argS 362933..364669 (-) 1737 WP_182816988.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35922.52 Da        Isoelectric Point: 6.8478

>NTDB_id=77261 H7K64_RS01685 WP_182816986.1 359805..360803(+) (pilT) [Pseudomonas putida strain WP8-W18-CRE-01]
MDVTDLLARAVAAGACDLHLATGEVPVLRLDGELQRLDLPPLAAATLNDGFAPLLDEAQRRQWAQGDELDIALILPGAVR
VRANLYRQHRGPGVSLRLLPARIPRLDELGLEEVFQAIAQWRDGLVLVGGPTGSGKSSTLAALIDQLNRTQALHIVTLED
PIEVIHSGQRSLVNQREIGRDCRNFAQGLRSALRQDPDVVVIGELRDLETIRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAPEEKALVRGMLAESLRMVVAQTLIRRLDGGRVAAREVLVATPAVRNLIREGRMAQLHSVMQTGAAAGMRTMEMAM
QQLQGAGLIASG

Nucleotide


Download         Length: 999 bp        

>NTDB_id=77261 H7K64_RS01685 WP_182816986.1 359805..360803(+) (pilT) [Pseudomonas putida strain WP8-W18-CRE-01]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGCTGCGGGCGCTTGCGACCTGCACCTGGCGACGGGCGAAGTACCGGT
GCTGCGCCTGGACGGCGAGCTGCAGCGCCTGGATCTGCCGCCTCTGGCGGCTGCGACCCTGAACGATGGCTTTGCTCCGC
TGCTCGATGAAGCCCAGCGCCGCCAGTGGGCGCAGGGCGATGAGTTGGATATCGCATTGATACTGCCAGGCGCCGTGCGC
GTGCGTGCGAACCTCTATCGCCAGCATCGTGGGCCAGGCGTGAGCCTGCGACTGCTGCCGGCTCGCATTCCCAGGCTCGA
TGAGCTGGGCCTAGAGGAAGTGTTTCAAGCGATTGCCCAGTGGCGCGATGGCCTCGTGCTGGTCGGCGGGCCGACAGGCA
GCGGCAAGTCATCGACCCTGGCGGCTCTGATCGATCAGCTCAATCGAACGCAGGCGCTGCACATCGTCACCCTCGAAGAC
CCCATCGAAGTTATCCACAGCGGCCAGCGCAGTCTGGTCAACCAGCGCGAGATCGGCCGCGACTGCCGAAATTTTGCCCA
AGGCCTGCGCAGTGCATTGCGCCAGGATCCGGATGTAGTCGTGATCGGCGAGCTGCGTGATCTGGAAACCATCCGCCTGG
CCTTGCGCGCTGCGGAAACGGGGCACCTGGTGTTGGCGACGGTGCATACCCGCTCGGCGGTGAACAGCATCGACCGCCTG
GTGGAGGTGTTTGCGCCAGAGGAAAAGGCTCTGGTGCGGGGCATGCTGGCGGAGTCGCTGCGCATGGTGGTGGCGCAGAC
ATTGATCAGACGATTGGATGGCGGGCGTGTGGCGGCCAGGGAAGTGCTGGTGGCTACGCCCGCCGTACGCAACCTGATCC
GTGAAGGTCGCATGGCGCAGTTGCATTCAGTGATGCAGACCGGAGCGGCGGCGGGGATGAGGACGATGGAGATGGCGATG
CAGCAATTGCAGGGGGCGGGGCTGATCGCGTCAGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

49.394

99.398

0.491
  pilT Neisseria meningitidis 8013

48.943

99.699

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Acinetobacter baylyi ADP1

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria gonorrhoeae MS11

48.64

99.699

0.485
  pilT Acinetobacter nosocomialis M2

48.182

99.398

0.479
  pilT Acinetobacter baumannii D1279779

48.182

99.398

0.479
  pilT Acinetobacter baumannii strain A118

48.182

99.398

0.479
  pilT Legionella pneumophila strain ERS1305867

46.667

99.398

0.464
  pilT Legionella pneumophila strain Lp02

46.667

99.398

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.484

95.783

0.455
  pilU Pseudomonas stutzeri DSM 10701

36.254

99.699

0.361

Multiple sequence alignment