Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M8445_RS06330 Genome accession   NZ_CP115165
Coordinates   1288833..1290113 (+) Length   426 a.a.
NCBI ID   WP_273990472.1    Uniprot ID   -
Organism   Deinococcus aquaticus strain PB-314     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1283833..1295113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8445_RS06315 (M8445_06315) pgeF 1284680..1285471 (+) 792 WP_273990468.1 peptidoglycan editing factor PgeF -
  M8445_RS06320 (M8445_06320) - 1285468..1285974 (+) 507 WP_273990469.1 YqeG family HAD IIIA-type phosphatase -
  M8445_RS06325 (M8445_06325) pilB 1286099..1288777 (+) 2679 WP_273990470.1 type II/IV secretion system protein Machinery gene
  M8445_RS06330 (M8445_06330) pilT 1288833..1290113 (+) 1281 WP_273990472.1 type IV pilus twitching motility protein PilT Machinery gene
  M8445_RS06335 (M8445_06335) lon 1290194..1292629 (-) 2436 WP_273990473.1 endopeptidase La -
  M8445_RS06340 (M8445_06340) clpX 1292768..1293976 (-) 1209 WP_273990474.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  M8445_RS06345 (M8445_06345) clpP 1293973..1294578 (-) 606 WP_273990475.1 ATP-dependent Clp protease proteolytic subunit -

Sequence

Protein


Download         Length: 426 a.a.        Molecular weight: 45776.12 Da        Isoelectric Point: 7.4182

>NTDB_id=768511 M8445_RS06330 WP_273990472.1 1288833..1290113(+) (pilT) [Deinococcus aquaticus strain PB-314]
MTQPAADITDILRFAADKGASDVILTVGLPPKFKLQGVYDSQGFTELAATETRKLMYSMMNEKQQRTFEERRELDFSFAL
GEKARFRVNAFMQRGNVGGVLRLIPTKIKSAQEMGLPQNVIDIANAPRGLVLVTGPTGSGKSTTLAAMIDYINTTKNLHI
MTIEDPIEFMHTHKQCVINQREVGADTMSFNDALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQSQIRVQLANNLVAVMTQQLLPRLDGQGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRVISFDVGVERSVDSKEFARLANDPTVGTAGGSAAAPAGYGSAPVAGFAPSTAPAPGGYAGRDAGAGR
GADTGRSASTTSTPEMGGGTSGYGRR

Nucleotide


Download         Length: 1281 bp        

>NTDB_id=768511 M8445_RS06330 WP_273990472.1 1288833..1290113(+) (pilT) [Deinococcus aquaticus strain PB-314]
ATGACCCAGCCCGCCGCCGACATCACCGACATCCTGCGTTTCGCCGCCGACAAGGGCGCGTCCGACGTGATCCTGACCGT
GGGACTCCCCCCGAAGTTCAAGTTGCAGGGCGTGTACGACTCGCAGGGGTTCACGGAACTGGCCGCCACCGAGACCCGCA
AACTGATGTACTCCATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAACGCCGCGAACTGGACTTCTCGTTCGCGCTG
GGCGAGAAGGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTGCTGCGACTGATCCCCACCAA
GATCAAGAGCGCCCAGGAGATGGGCCTGCCGCAGAACGTCATCGACATCGCCAACGCGCCGCGCGGACTGGTGCTCGTGA
CCGGCCCGACCGGCTCGGGGAAATCCACCACGCTGGCCGCCATGATCGATTACATCAACACCACCAAGAACCTGCACATC
ATGACCATCGAGGATCCCATCGAGTTCATGCACACGCACAAGCAGTGCGTCATCAACCAGCGTGAGGTCGGTGCGGATAC
CATGAGCTTCAACGACGCCCTGCGCGCTGTGCTGCGTCAGGCCCCGGACGTCATCCTGGTCGGCGAAATGCGCGACTACG
AGACCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGTCACCTCGTGATGGGCACCCTGCACACCAACTCTGCCCCGGAA
TCCATCGACCGTATCGTGGACGTGTTCCCGGAAGAGCAGCAGTCGCAGATCCGCGTGCAGCTCGCCAACAACCTCGTGGC
CGTCATGACCCAGCAGCTGCTGCCCCGCCTGGACGGACAGGGTCGCATCCTGGCGTACGAACTGCTGATCGCCAACCCCG
CTGTGCGCGCCCTGATCCGCGAGGGCAAGACCTACCAGATCACCAGTGTCATGCAGACTGGCGCGCGCGAGGGCATGGTC
ACCATGGACGCCTTCCTGGCGAACCTGTACCGCCGCCGCGTGATCTCCTTCGACGTGGGCGTGGAACGCTCCGTGGACAG
CAAGGAATTCGCCCGCCTCGCCAACGACCCCACGGTCGGCACGGCCGGCGGCAGCGCCGCCGCGCCCGCCGGGTACGGCA
GCGCGCCCGTGGCTGGCTTCGCGCCCAGCACCGCCCCCGCACCCGGCGGGTACGCGGGCCGTGACGCCGGAGCCGGGCGC
GGCGCGGATACCGGACGCAGCGCCAGCACCACCAGCACCCCCGAAATGGGCGGCGGCACCAGCGGGTACGGACGCCGCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(33-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

73.023

100

0.737
  pilT Neisseria meningitidis 8013

51.437

81.69

0.42
  pilT Pseudomonas stutzeri DSM 10701

51.744

80.751

0.418
  pilT Acinetobacter nosocomialis M2

53.636

77.465

0.415
  pilT Acinetobacter baumannii D1279779

53.636

77.465

0.415
  pilT Acinetobacter baumannii strain A118

53.636

77.465

0.415
  pilT Pseudomonas aeruginosa PAK

50.872

80.751

0.411
  pilT Neisseria gonorrhoeae MS11

50.872

80.751

0.411
  pilT Acinetobacter baylyi ADP1

52.568

77.7

0.408
  pilT Legionella pneumophila strain Lp02

48.837

80.751

0.394
  pilT Legionella pneumophila strain ERS1305867

48.837

80.751

0.394
  pilT Vibrio cholerae strain A1552

50.456

77.23

0.39
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

77.23

0.39