Detailed information NTG0768510

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: M8445_RS06325
• Genome accession: NZ_CP115165
• Coordinates: 1286099..1288777 (+)
• Length: 892 a.a.
• NCBI ID: WP_273990470.1
• Uniprot ID: -
• Organism: Deinococcus aquaticus strain PB-314
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1281099..1293777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8445_RS06295 (M8445_06295)	-	1281735..1282148 (-)	414	WP_273990463.1	isochorismatase family protein	-
M8445_RS06300 (M8445_06300)	-	1282199..1283131 (-)	933	WP_273990464.1	NADP-dependent oxidoreductase	-
M8445_RS06305 (M8445_06305)	-	1283319..1283696 (+)	378	WP_273990465.1	winged helix-turn-helix transcriptional regulator	-
M8445_RS06310 (M8445_06310)	-	1283746..1284534 (-)	789	WP_273990467.1	enoyl-ACP reductase FabI	-
M8445_RS06315 (M8445_06315)	pgeF	1284680..1285471 (+)	792	WP_273990468.1	peptidoglycan editing factor PgeF	-
M8445_RS06320 (M8445_06320)	-	1285468..1285974 (+)	507	WP_273990469.1	YqeG family HAD IIIA-type phosphatase	-
M8445_RS06325 (M8445_06325)	pilB	1286099..1288777 (+)	2679	WP_273990470.1	type II/IV secretion system protein	Machinery gene
M8445_RS06330 (M8445_06330)	pilT	1288833..1290113 (+)	1281	WP_273990472.1	type IV pilus twitching motility protein PilT	Machinery gene
M8445_RS06335 (M8445_06335)	lon	1290194..1292629 (-)	2436	WP_273990473.1	endopeptidase La	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97361.26 Da
• Isoelectric Point: 4.9438

>NTDB_id=768510 M8445_RS06325 WP_273990470.1 1286099..1288777(+) (pilB) [Deinococcus aquaticus strain PB-314]
MALSIGDRRLGAILLEQGYVNDTDLQKALVRHAEVGGRLAEILIDSGMVGEKRIARAIEEALGIPLVNLLVVTPEPQALA
AVRSQTALSVQAFPFAVEGGTLRVAIVDPLSSLSIEALEDDSGLNIEPYQALRDQIMWAIATNYPDLNLQADLPADAEGN
SGGGMMGQRLISRGLITDAQLQIALDSQQQTGEPLGSTLIAQKSITEDQLYEVLAEQVGAVYLRNPRDFQPSEDVLGSML
RADALRLNAVPVDETDSGVTVVASDPRKREDIEALVGRPVQLILAKPRDVELLIERFYPQRGRLGEQMVQQGTLSRAQLR
EALQVQAREGRVKPLGEVIVELGFAGADEIDNALQKQNSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
SKVALMIPESTARRYGVVPVRLQGESLVIAMKDPRNVFALDDLKLITGREVIPAVMAEKDIVRLIERYFGSKDMADLNQR
LVQESNKRDKDNKRQEDTDVTAGLDDNAVVRVVDNIIREAALQEASDIHIEPTETALRVRYRVDGILREQNELPKGSSQS
ILARIKIMGHLDISERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNYQRYLDTIH
KPNGIFLVTGPTGSGKSFTSFSTLKRIAVPEKNTTTIEDPVEYEIPGIIQSQVNNTAGMTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEHFNIGAAVVGVVAQRLVRKVCPDCKAPTNADPDVLRRL
GITERDLRGAQLMRGAGCNRCGGTGYKGRMGIHELMVIDEPLRVAIGSGRNATEIAEVAMTQSGMKTLRQDGIEKALSGI
TTLEEVLAVTSK

Nucleotide

• Length: 2679 bp

>NTDB_id=768510 M8445_RS06325 WP_273990470.1 1286099..1288777(+) (pilB) [Deinococcus aquaticus strain PB-314]
ATGGCACTCTCAATCGGTGACCGGCGCCTGGGCGCCATTCTGCTCGAACAGGGGTACGTGAACGACACGGACCTGCAAAA
AGCCCTGGTCCGTCACGCCGAGGTGGGCGGCCGCCTCGCAGAGATCCTGATCGACTCCGGCATGGTCGGCGAGAAACGCA
TCGCGCGCGCCATCGAGGAGGCGCTGGGTATTCCGCTGGTGAACCTGCTGGTCGTGACGCCCGAACCGCAGGCCCTGGCC
GCCGTGCGCTCCCAGACGGCCCTGAGCGTGCAGGCCTTCCCGTTCGCCGTGGAGGGCGGCACCCTGCGCGTGGCGATCGT
GGACCCGCTGTCCAGCCTGAGCATCGAGGCGCTCGAGGACGACAGCGGCCTGAACATCGAGCCGTACCAGGCGCTGCGCG
ACCAGATCATGTGGGCCATCGCCACGAACTACCCGGACCTGAACCTGCAGGCCGACCTGCCTGCCGACGCCGAGGGTAAC
AGTGGCGGCGGCATGATGGGCCAGCGCCTGATCTCGCGCGGCCTGATCACGGACGCCCAGCTTCAGATTGCGCTGGACTC
GCAGCAGCAGACGGGCGAACCGCTGGGCTCCACCCTGATCGCGCAGAAATCCATCACCGAGGATCAACTGTACGAGGTGC
TGGCCGAACAGGTCGGCGCGGTGTACCTGCGTAACCCCCGCGACTTCCAGCCCAGTGAGGACGTGCTGGGCAGCATGCTG
CGCGCCGACGCGCTGCGCCTGAACGCCGTGCCCGTCGACGAGACCGACTCGGGCGTCACCGTGGTCGCCAGCGATCCCCG
CAAACGCGAGGACATCGAGGCGCTGGTGGGCCGCCCCGTGCAGCTGATCCTCGCCAAGCCCCGCGACGTGGAACTGCTGA
TCGAACGCTTCTACCCGCAGCGCGGCCGCCTGGGCGAGCAGATGGTGCAGCAGGGCACCCTGTCACGCGCGCAACTGCGC
GAGGCGCTGCAGGTGCAGGCCCGCGAGGGCCGCGTCAAACCGCTGGGCGAGGTGATCGTGGAACTCGGCTTCGCCGGGGC
CGACGAGATCGACAACGCCCTGCAGAAACAGAACTCCGGGGGCGGCCGACTGGAAGACACGCTGGTGCAGTCCGGGAAGC
TCAGCCCGGAAATGCTGGCCCGCTCGCTGGCTGCGCAGCTCGGCTACGAGTTCCTCGACCCGGTTCAGAACCCGCCGGAC
AGCAAGGTCGCGCTGATGATCCCGGAAAGCACCGCCCGCCGCTACGGCGTGGTCCCGGTCCGCCTTCAGGGCGAGTCTCT
GGTCATCGCCATGAAGGACCCGCGCAATGTGTTCGCGCTGGACGACCTGAAACTCATCACGGGCCGCGAGGTCATCCCGG
CCGTCATGGCCGAAAAGGACATCGTCCGCCTGATCGAGCGGTACTTCGGCAGCAAGGACATGGCCGACCTGAACCAGAGG
CTGGTGCAGGAAAGCAACAAGCGCGACAAGGACAACAAACGCCAGGAAGACACCGACGTCACCGCCGGACTGGACGACAA
CGCCGTGGTGCGCGTCGTGGACAACATCATCCGCGAGGCGGCCCTGCAGGAAGCCAGTGACATCCACATCGAACCGACCG
AGACGGCCCTGCGCGTCCGCTACCGCGTGGACGGCATCCTGCGCGAGCAGAACGAGCTTCCCAAGGGCAGTTCGCAGAGC
ATCCTGGCGCGCATCAAGATCATGGGCCACCTGGACATCAGCGAGCGGCGCGTGCCGCAGGACGGCCGCGTGCGCTTCAA
GAAGGGCAGCATCGACCTCGACCTGCGACTGTCCACGCTGCCCACCGTGTACGGCGAGAAGGCCGTCATGCGACTGCTGC
AGAAGGCCAGCAACATCCCGGAAGTCGAGCAGCTGGGCTTTTCCGAACACAACTACCAGCGCTACCTGGACACCATCCAC
AAACCCAACGGCATCTTCCTGGTGACCGGCCCCACGGGTTCCGGGAAGTCCTTCACCAGCTTCTCGACCCTCAAGCGCAT
CGCGGTTCCCGAGAAGAACACCACCACCATCGAGGACCCGGTCGAGTACGAGATTCCGGGCATCATCCAGTCGCAGGTGA
ACAACACGGCGGGCATGACCTTCGCCCGTGCCCTGCGAGCCTTCCTGCGACAGGATCCCGACATCATCTTCGTTGGCGAG
ATCCGAGACACCGAAACCGCCAAGATCGCCGTCGAGGCCGCCCTGACCGGACACCTCGTGCTGGCCACCCTGCACACCAA
CGACGCGCCCGGCGCGATCGTGCGACTGGAAGAGATGGGCGTCGAGCACTTCAACATCGGCGCGGCCGTCGTGGGCGTGG
TCGCCCAGCGCCTCGTGCGTAAGGTCTGCCCGGACTGCAAGGCCCCCACCAACGCCGACCCGGACGTGCTGCGCCGCCTG
GGCATCACGGAACGCGACCTGCGCGGCGCGCAACTGATGCGCGGGGCCGGCTGCAACCGCTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGTATCCACGAACTGATGGTCATCGATGAGCCGCTGCGCGTGGCCATCGGTTCAGGCAGGAACGCCACCG
AGATCGCCGAGGTCGCCATGACCCAGAGCGGCATGAAAACCCTGCGCCAGGACGGCATCGAGAAGGCGCTGTCCGGCATC
ACCACCCTCGAGGAGGTGCTGGCCGTCACCAGCAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (516-781)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	77.578	100	0.776
pilF	Thermus thermophilus HB27	54.413	100	0.546