NTDB

Overview

Name	pilA/pilA1	Type	Machinery gene
Locus tag	OU419_RS26885	Genome accession	NZ_CP113432
Coordinates	5809840..5810265 (-)	Length	141 a.a.
NCBI ID	WP_302329068.1	Uniprot ID	-
Organism	Pseudomonas triclosanedens strain ZM23
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5804840..5815265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OU419_RS26865 (OU419_26865)	-	5805277..5805816 (+)	540	WP_254475191.1	type 1 glutamine amidotransferase domain-containing protein	-
OU419_RS26870 (OU419_26870)	-	5805903..5807099 (+)	1197	WP_254475192.1	class I SAM-dependent rRNA methyltransferase	-
OU419_RS26875 (OU419_26875)	-	5807182..5807508 (-)	327	WP_254475194.1	hypothetical protein	-
OU419_RS26880 (OU419_26880)	ilvD	5807870..5809708 (+)	1839	WP_254475196.1	dihydroxy-acid dehydratase	-
OU419_RS26885 (OU419_26885)	pilA/pilA1	5809840..5810265 (-)	426	WP_302329068.1	pilin	Machinery gene
OU419_RS26895 (OU419_26895)	-	5810585..5811976 (+)	1392	WP_254475198.1	nucleobase:cation symporter-2 family protein	-
OU419_RS26900 (OU419_26900)	-	5812026..5812493 (+)	468	WP_254475200.1	metallophosphoesterase family protein	-
OU419_RS26905 (OU419_26905)	-	5812678..5814051 (-)	1374	WP_254475202.1	GTPase/DUF3482 domain-containing protein	-

Sequence

Protein

Download Length: 141 a.a. Molecular weight: 14565.68 Da Isoelectric Point: 8.4829

                                >NTDB_id=761965 OU419_RS26885 WP_302329068.1 5809840..5810265(-) (pilA/pilA1) [Pseudomonas triclosanedens strain ZM23]

MKSMQKGFTLIELMIVIAIIGILASIAFPAYQDYTARSQAVEALQATAGIQGDVAVDTANAGSLTATTDTIAAAQDLEGK

YFKAKSASVTSGGIISVKFDSGALNGQTMTLTPTLNAAKNQIASWKCTGLSKNNTLPSGCR

Nucleotide

Download Length: 426 bp

                                >NTDB_id=761965 OU419_RS26885 WP_302329068.1 5809840..5810265(-) (pilA/pilA1) [Pseudomonas triclosanedens strain ZM23]

ATGAAAAGTATGCAGAAGGGTTTTACCCTTATCGAACTGATGATCGTAATTGCGATTATCGGTATTCTGGCCTCGATTGC

CTTCCCGGCATATCAGGATTACACGGCTCGCTCCCAGGCTGTCGAGGCATTGCAGGCGACGGCGGGAATCCAGGGTGATG

TAGCTGTCGACACCGCAAATGCAGGATCCTTGACGGCGACGACAGATACCATTGCTGCAGCCCAAGACCTTGAGGGGAAG

TATTTCAAGGCTAAGAGCGCCAGTGTTACGAGTGGCGGGATTATAAGTGTGAAGTTTGATTCTGGCGCCCTGAATGGTCA

AACAATGACTTTGACTCCAACGCTGAATGCTGCAAAAAACCAGATTGCTAGCTGGAAGTGCACCGGCTTGTCTAAGAATA

ATACCCTTCCATCCGGGTGTCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilA1	Eikenella corrodens VA1	50	100	0.539
pilA2	Legionella pneumophila str. Paris	43.836	100	0.454
pilA2	Legionella pneumophila strain ERS1305867	43.537	100	0.454
comP	Acinetobacter baylyi ADP1	42.466	100	0.44
pilE	Neisseria gonorrhoeae strain FA1090	37.654	100	0.433
pilE	Neisseria gonorrhoeae MS11	36.646	100	0.418
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.716	100	0.397
pilA	Ralstonia pseudosolanacearum GMI1000	36.667	100	0.39
pilA	Pseudomonas aeruginosa PAK	36.913	100	0.39
pilA	Haemophilus influenzae 86-028NP	41.985	92.908	0.39
pilA	Glaesserella parasuis strain SC1401	39.13	97.872	0.383
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.111	100	0.369
pilA	Vibrio cholerae C6706	36.111	100	0.369
pilA	Vibrio cholerae strain A1552	36.111	100	0.369

Detailed information NTG0761965