Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OSW16_RS01850 Genome accession   NZ_CP113097
Coordinates   380735..381733 (+) Length   332 a.a.
NCBI ID   WP_267820331.1    Uniprot ID   -
Organism   Pseudomonas putida strain WYTS.T61     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375735..386733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSW16_RS01820 (OSW16_01820) - 376212..376646 (-) 435 WP_267820322.1 DUF4426 domain-containing protein -
  OSW16_RS01825 (OSW16_01825) metW 376669..377289 (-) 621 WP_241805087.1 methionine biosynthesis protein MetW -
  OSW16_RS01830 (OSW16_01830) metX 377297..378436 (-) 1140 WP_267820325.1 homoserine O-succinyltransferase MetX -
  OSW16_RS01835 (OSW16_01835) - 378525..379115 (-) 591 WP_012312312.1 YggT family protein -
  OSW16_RS01840 (OSW16_01840) proC 379126..379944 (-) 819 WP_267820327.1 pyrroline-5-carboxylate reductase -
  OSW16_RS01845 (OSW16_01845) - 379983..380669 (-) 687 WP_267820329.1 YggS family pyridoxal phosphate-dependent enzyme -
  OSW16_RS01850 (OSW16_01850) pilT 380735..381733 (+) 999 WP_267820331.1 type IV pilus twitching motility protein PilT Machinery gene
  OSW16_RS01855 (OSW16_01855) - 381769..382395 (-) 627 WP_267820333.1 C40 family peptidase -
  OSW16_RS01860 (OSW16_01860) - 382572..382991 (+) 420 WP_267820335.1 NINE protein -
  OSW16_RS01865 (OSW16_01865) - 383036..383734 (-) 699 WP_267820337.1 SPOR domain-containing protein -
  OSW16_RS01870 (OSW16_01870) argS 383736..385472 (-) 1737 WP_267820339.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36111.73 Da        Isoelectric Point: 9.2807

>NTDB_id=760168 OSW16_RS01850 WP_267820331.1 380735..381733(+) (pilT) [Pseudomonas putida strain WYTS.T61]
MDVTDLLARAGDAGASDLHLAAGEIPMLRVEGELRRMALPSMTPLALTNALAPLLDERRRQQWAQGDEQDLALELPPLGR
FRLNLFRQLNGPAATFRLIPKRIATLDELDLEDVFRSVAQWTDGLILLGGPTGSGKSSTLAALVDQLNRQRPLHIITLED
PVEVIHSSQRSLVNQREIGRHCRDFSQGLRSALRQDPDVIVIGELRDLETIRLALRASETGHLVLATVHTRSAAGSVDRL
VDVFAAEEKPLVRAMLAESLRMVVAQVLVKRAGGGRVAAREVLVGTSAVRNLIREGRMAQLNSVMQAGAAQGMRTMEGAL
QRLKAQGLIGAV

Nucleotide


Download         Length: 999 bp        

>NTDB_id=760168 OSW16_RS01850 WP_267820331.1 380735..381733(+) (pilT) [Pseudomonas putida strain WYTS.T61]
ATGGACGTGACTGACCTGTTGGCTCGGGCTGGCGATGCAGGGGCGAGCGACCTGCACCTGGCCGCGGGCGAGATACCGAT
GCTGCGCGTGGAGGGTGAACTGCGGCGCATGGCGCTACCGAGCATGACGCCTTTGGCGTTGACCAATGCCCTGGCGCCGC
TGCTCGATGAGCGCCGGCGCCAGCAGTGGGCGCAAGGTGACGAGCAGGACCTGGCACTGGAACTGCCCCCGCTTGGGCGC
TTTCGCCTGAACCTGTTTCGCCAGTTGAATGGCCCGGCGGCCACCTTTCGCTTGATCCCGAAGCGGATCGCGACACTCGA
CGAGCTCGACCTTGAAGATGTGTTTCGTTCTGTTGCGCAGTGGACTGACGGCCTGATTCTGCTCGGTGGGCCTACCGGCA
GTGGTAAGTCCAGCACGTTGGCGGCGCTGGTCGATCAGCTGAACCGGCAGCGGCCCTTGCACATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGAAGCCTGGTCAATCAGCGTGAGATCGGTCGACACTGCCGCGATTTTTCCCA
AGGGTTGCGCAGCGCCTTGCGTCAGGACCCGGACGTGATCGTCATAGGCGAATTGCGCGACCTGGAGACGATTCGCCTGG
CGTTGCGGGCTTCGGAGACCGGGCACCTGGTGTTGGCAACCGTACATACGCGCTCGGCTGCCGGTAGTGTCGACCGGCTG
GTGGACGTGTTTGCCGCCGAGGAGAAACCGCTGGTGCGGGCGATGTTGGCGGAGTCATTGCGCATGGTGGTTGCGCAGGT
GCTGGTCAAGCGTGCGGGGGGCGGGCGGGTGGCAGCCCGGGAGGTGCTGGTGGGTACGTCTGCGGTGCGCAACCTCATCC
GCGAGGGGCGCATGGCGCAATTGAATTCGGTGATGCAGGCGGGTGCTGCGCAGGGTATGCGCACGATGGAGGGGGCATTG
CAGCGATTGAAGGCGCAGGGATTGATCGGGGCCGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.672

99.096

0.512
  pilT Acinetobacter baylyi ADP1

51.515

99.398

0.512
  pilT Pseudomonas stutzeri DSM 10701

51.368

99.096

0.509
  pilT Neisseria meningitidis 8013

51.057

99.699

0.509
  pilT Neisseria gonorrhoeae MS11

50.755

99.699

0.506
  pilT Vibrio cholerae strain A1552

49.849

99.699

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.849

99.699

0.497
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter nosocomialis M2

49.848

99.096

0.494
  pilT Legionella pneumophila strain Lp02

48.485

99.398

0.482
  pilT Legionella pneumophila strain ERS1305867

48.485

99.398

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.636

99.398

0.434
  pilU Pseudomonas stutzeri DSM 10701

37.273

99.398

0.37