Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   OQB65_RS03975 Genome accession   NZ_CP110808
Coordinates   891522..891953 (+) Length   143 a.a.
NCBI ID   WP_103694589.1    Uniprot ID   A0AAE5S4P4
Organism   Pseudomonas syringae strain MUP20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 886522..896953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQB65_RS03950 (OQB65_03950) yacG 886681..886890 (-) 210 WP_024960618.1 DNA gyrase inhibitor YacG -
  OQB65_RS03955 (OQB65_03955) coaE 886887..887510 (-) 624 WP_266001758.1 dephospho-CoA kinase -
  OQB65_RS03960 (OQB65_03960) pilD 887507..888379 (-) 873 WP_024638544.1 prepilin peptidase Machinery gene
  OQB65_RS03965 (OQB65_03965) pilC 888381..889598 (-) 1218 WP_100073388.1 type II secretion system F family protein Machinery gene
  OQB65_RS03970 (OQB65_03970) pilB 889601..891295 (-) 1695 WP_103694577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OQB65_RS03975 (OQB65_03975) pilA2 891522..891953 (+) 432 WP_103694589.1 pilin Machinery gene
  OQB65_RS26395 - 892214..892428 (-) 215 Protein_784 winged helix-turn-helix transcriptional regulator -
  OQB65_RS03980 (OQB65_03980) - 892638..893330 (+) 693 WP_338129179.1 DUF433 domain-containing protein -
  OQB65_RS03985 (OQB65_03985) - 893378..893923 (-) 546 WP_010415546.1 DUF1523 family protein -
  OQB65_RS03990 (OQB65_03990) - 894043..895041 (+) 999 WP_266001764.1 ATP-grasp domain-containing protein -
  OQB65_RS03995 (OQB65_03995) - 895096..896235 (+) 1140 WP_003414836.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14553.83 Da        Isoelectric Point: 9.1226

>NTDB_id=756282 OQB65_RS03975 WP_103694589.1 891522..891953(+) (pilA2) [Pseudomonas syringae strain MUP20]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRSKVTEGLTQAASAKLAVAETAANVGGVANVTAANSGFVFNQVAG
PNSYVAAVAVGAGGAITVTTKETGATVQPIFNLNPVQASPNDQITWTCVRVAGLAKHLPANCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=756282 OQB65_RS03975 WP_103694589.1 891522..891953(+) (pilA2) [Pseudomonas syringae strain MUP20]
ATGAAAGCACAAAAAGGTTTTACATTGATCGAATTGATGATCGTTGTAGCGATTATCGGTATTTTGGCGGCGGTTGCGCT
GCCTGCCTATCAGGACTACACAATCAGAAGTAAGGTTACTGAAGGTTTGACGCAGGCGGCTTCAGCAAAGCTGGCAGTTG
CCGAAACTGCTGCGAACGTTGGCGGTGTTGCTAACGTCACGGCTGCGAACTCAGGGTTTGTATTTAACCAAGTTGCTGGT
CCGAACTCATACGTTGCTGCCGTGGCTGTAGGGGCGGGCGGTGCTATCACTGTTACTACCAAGGAAACCGGAGCTACAGT
ACAGCCCATCTTCAACCTCAATCCGGTGCAAGCTTCCCCTAACGACCAGATAACCTGGACTTGCGTAAGGGTTGCCGGCC
TTGCCAAGCACCTTCCTGCTAACTGCAGGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

56.738

98.601

0.559
  pilA2 Legionella pneumophila strain ERS1305867

56.028

98.601

0.552
  pilA Ralstonia pseudosolanacearum GMI1000

42.105

100

0.503
  comP Acinetobacter baylyi ADP1

45.098

100

0.483
  pilA/pilA1 Eikenella corrodens VA1

39.103

100

0.427
  pilE Neisseria gonorrhoeae strain FA1090

38.462

100

0.42
  pilE Neisseria gonorrhoeae MS11

37.821

100

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.406
  pilA Acinetobacter baumannii strain A118

39.583

100

0.399
  pilA Haemophilus influenzae 86-028NP

37.857

97.902

0.371
  pilA Haemophilus influenzae Rd KW20

37.857

97.902

0.371