Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OQB65_RS03970 Genome accession   NZ_CP110808
Coordinates   889601..891295 (-) Length   564 a.a.
NCBI ID   WP_103694577.1    Uniprot ID   A0AAE5VSU3
Organism   Pseudomonas syringae strain MUP20     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884601..896295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQB65_RS03935 (OQB65_03935) - 884809..885342 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  OQB65_RS03940 (OQB65_03940) - 885339..885968 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  OQB65_RS03945 (OQB65_03945) - 886094..886555 (+) 462 WP_003345128.1 hypothetical protein -
  OQB65_RS03950 (OQB65_03950) yacG 886681..886890 (-) 210 WP_024960618.1 DNA gyrase inhibitor YacG -
  OQB65_RS03955 (OQB65_03955) coaE 886887..887510 (-) 624 WP_266001758.1 dephospho-CoA kinase -
  OQB65_RS03960 (OQB65_03960) pilD 887507..888379 (-) 873 WP_024638544.1 prepilin peptidase Machinery gene
  OQB65_RS03965 (OQB65_03965) pilC 888381..889598 (-) 1218 WP_100073388.1 type II secretion system F family protein Machinery gene
  OQB65_RS03970 (OQB65_03970) pilB 889601..891295 (-) 1695 WP_103694577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OQB65_RS03975 (OQB65_03975) pilA2 891522..891953 (+) 432 WP_103694589.1 pilin Machinery gene
  OQB65_RS26395 - 892214..892428 (-) 215 Protein_784 winged helix-turn-helix transcriptional regulator -
  OQB65_RS03980 (OQB65_03980) - 892638..893330 (+) 693 WP_338129179.1 DUF433 domain-containing protein -
  OQB65_RS03985 (OQB65_03985) - 893378..893923 (-) 546 WP_010415546.1 DUF1523 family protein -
  OQB65_RS03990 (OQB65_03990) - 894043..895041 (+) 999 WP_266001764.1 ATP-grasp domain-containing protein -
  OQB65_RS03995 (OQB65_03995) - 895096..896235 (+) 1140 WP_003414836.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62524.76 Da        Isoelectric Point: 6.2549

>NTDB_id=756281 OQB65_RS03970 WP_103694577.1 889601..891295(-) (pilB) [Pseudomonas syringae strain MUP20]
MTDAVLTGLAKQLVLAELLTESVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASEQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRDTLIQEGFPEQK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=756281 OQB65_RS03970 WP_103694577.1 889601..891295(-) (pilB) [Pseudomonas syringae strain MUP20]
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTACTTACTGAATCTGTTGCGCAACAAGC
GTATCAACAGGCGCGCCGTGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAATTAGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGAGCAGTTTGGCGTGCCATTCATGGATCTCGCCAGCCTTGACAAGGAAAGCCAGCCTAAAGGATTG
GTCAGTGAAAAACTGGTTCGCCAACATCACGCGCTGCCCCTGTGGCGACGCGGTAACAAGCTATTTATCGGAATTTCAGA
TCCGACCAATCATCAGGCGGTCACCGATATTCAGTTCAGTACTGGCCTGAATACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACTACCGCGATAGATCGATTCTTTGACAGTGATAGCGGTCTGGGCAACCTGGAAGATGTGGATCTGGGTCTGGAT
ATTGAACCGGCTGATGGCAAGGAAACTTCCCTCGCTACGCAGAATGACGCAGACGATGCCCCGGTGGTGCGCTTCGTCAA
CAAAATGTTGATGGACGCTATTCGGTTAGGATCGTCCGACCTGCACTTTGAGCCTTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACCGACGGCATCTTGCATGAAGTCGCTAGACCACCAATTCATTTGGCGAACCGTATCGCCGCGCGCTTGAAAGTG
ATGGCCAGCCTGGATATCTCGGAACGACGCAAACCGCAGGACGGACGCGTCAAGTTACGTGTGTCCAAAACAAAAGCCAT
CGATTTTCGTATGAACACCTTACCCACGTTGTGGGGCGAGAAGATCGTAATGCGGATCCTCGATCCGACCAGTGCGCAGA
TGGGCATTGATGCGCTGGGCTACGAGCCAGAACAAAAGGCATTGTATCTGGAGGCGCTGAAGCAGCCGCAAGGTATGATT
CTCGTAACCGGCCCGACAGGCTCCGGTAAGACAGTGTCCCTGTATACCGGGCTGAATATACTCAATACCGTGGATATCAA
TATTTCTACAGCCGAAGATCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGCCAGGGACTGG
ACTTTTCCCAAGCCTTGCGCGCATTTTTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACCTGGAAACT
GCCGAAATCGCCATCAAGGCCTCGCAGACTGGCCATATGGTGCTCTCAACTCTGCACACCAACAGCGCCGCGGAAACGCT
GACTCGCCTGCACCATATGGGCGTTGCAGCATTCAATATTGCAACAGCGATCAACCTGATTATTGCGCAACGACTGGCGC
GCAAGCTGTGTAGCCACTGCAAAAAAGAACTGGATATCCCTCGCGACACACTGATTCAGGAAGGCTTTCCAGAACAGAAA
ATTGGCACCTTCAAGATCTATGGTCCGGTGGGCTGCGAGCACTGCAATGGTGGTTACAGGGGCCGAGTAGGTATTTACGA
AGTGGTCAAAAAAACACCGGAGCTGGAGCGCATCATCATGGAAGAAGGCAACTCATTGGAAATATCCAGACAAATGCGCA
AGGACGGCTTCAATGACCTTCGCACATCGGGGCTTTCGAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.025

100

0.583
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

51.068

99.645

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.714

99.291

0.504
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

38.516

100

0.387