Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OQB65_RS03965 Genome accession   NZ_CP110808
Coordinates   888381..889598 (-) Length   405 a.a.
NCBI ID   WP_100073388.1    Uniprot ID   -
Organism   Pseudomonas syringae strain MUP20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883381..894598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQB65_RS03935 (OQB65_03935) - 884809..885342 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  OQB65_RS03940 (OQB65_03940) - 885339..885968 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  OQB65_RS03945 (OQB65_03945) - 886094..886555 (+) 462 WP_003345128.1 hypothetical protein -
  OQB65_RS03950 (OQB65_03950) yacG 886681..886890 (-) 210 WP_024960618.1 DNA gyrase inhibitor YacG -
  OQB65_RS03955 (OQB65_03955) coaE 886887..887510 (-) 624 WP_266001758.1 dephospho-CoA kinase -
  OQB65_RS03960 (OQB65_03960) pilD 887507..888379 (-) 873 WP_024638544.1 prepilin peptidase Machinery gene
  OQB65_RS03965 (OQB65_03965) pilC 888381..889598 (-) 1218 WP_100073388.1 type II secretion system F family protein Machinery gene
  OQB65_RS03970 (OQB65_03970) pilB 889601..891295 (-) 1695 WP_103694577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OQB65_RS03975 (OQB65_03975) pilA2 891522..891953 (+) 432 WP_103694589.1 pilin Machinery gene
  OQB65_RS26395 - 892214..892428 (-) 215 Protein_784 winged helix-turn-helix transcriptional regulator -
  OQB65_RS03980 (OQB65_03980) - 892638..893330 (+) 693 WP_338129179.1 DUF433 domain-containing protein -
  OQB65_RS03985 (OQB65_03985) - 893378..893923 (-) 546 WP_010415546.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43960.81 Da        Isoelectric Point: 10.0695

>NTDB_id=756280 OQB65_RS03965 WP_100073388.1 888381..889598(-) (pilC) [Pseudomonas syringae strain MUP20]
MASKAVKVNIYTWEGVDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPMFTLMVIGLSEVIQQWWIAIVVAFFASFFLFK
KAYKKSQNFRDSIDRILLKIPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRATGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGDVA

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=756280 OQB65_RS03965 WP_100073388.1 888381..889598(-) (pilC) [Pseudomonas syringae strain MUP20]
ATGGCCAGCAAGGCAGTTAAAGTCAATATCTATACTTGGGAAGGCGTAGATAAAAAAGGCGGAAAACTCAGCGGTGAATT
GAGCGGCCATAACCCGGCACTCATCAAGGCACAACTGCGTAAACAGGGTGTAAACCCTACCAAGGTGCGTAAAAAATCTG
CTTCTATCTTCGGTAAAGGTAAAAAAATAAAACCATTGGACATCGCCTTTTTTTCTCGGCAAATGGCGACAATGATGAAA
GCTGGCGTGCCGCTGCTACAGTCGTTCGACATTATTAGCGAGGGGGCAGAAAACCCAAATATGCGAGCATTGGTAAACTC
GCTCAAGCAGGAAGTTTCGGCGGGAAATAGTTTTGCAACAGCCTTACGGCAAAAGCCTGAGTACTTTGACGACCTGTTCT
GTAATTTAGTAGATGCGGGAGAGCAGGCGGGAGCACTCGAAAGTCTGTTGGACCGAGTCGCGACCTATAAAGAAAAAACA
GAAAAACTCAAAGCCAAAATCAAGAAAGCAATGACCTATCCTGCGGCCGTGTTGATCGTTGCGGTGATCGTATCAGGTAT
TCTGCTCATAAAAGTTGTACCACAATTTCAATCGGTTTTTGCGGGTTTTGGCGCCGAGTTGCCAATGTTCACATTGATGG
TTATCGGGCTTTCCGAGGTCATCCAGCAATGGTGGATAGCTATTGTTGTGGCGTTCTTTGCTAGCTTCTTTTTGTTCAAA
AAGGCTTATAAAAAATCACAGAATTTTCGAGATAGTATTGATCGAATATTGTTGAAGATCCCGGTTATCGGACCGTTGAT
TTTTAAGTCATCCGTTGCACGTTACGCACGAACACTGGCAACTACCTTCGCTGCCGGCGTTCCGCTTGTGGAAGCGCTGG
ACTCGGTCGCAGGTGCAACAGGTAATGTGGTTTTTAAAAATGCGGTAAATAAAGTTAAACAAGACGTGTCCACAGGGATG
CAGTTGAATTTTTCAATGCGTGCAACGGGAGTTTTTCCAAGCCTGGCAATCCAGATGACTGCGATCGGTGAAGAGTCCGG
TGCTCTGGACAACATGCTGGATAAGGTCGCGACCTACTATGAGGATGAAGTCGATAACATGGTTGATAGCCTGACCAGCC
TAATGGAGCCAATGATCATGGCCATACTCGGCGTCGTTGTCGGCGGCTTAGTGATTGCGATGTATTTGCCAATATTCAAA
CTAGGGGACGTTGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.762

99.753

0.736
  pilC Acinetobacter baylyi ADP1

58.968

100

0.593
  pilC Acinetobacter baumannii D1279779

59.305

99.506

0.59
  pilC Legionella pneumophila strain ERS1305867

52.926

97.037

0.514
  pilG Neisseria gonorrhoeae MS11

42.786

99.259

0.425
  pilG Neisseria meningitidis 44/76-A

42.537

99.259

0.422
  pilC Vibrio campbellii strain DS40M4

41.235

100

0.412
  pilC Vibrio cholerae strain A1552

41.337

99.753

0.412
  pilC Thermus thermophilus HB27

37.656

99.012

0.373