Detailed information NTG0756279

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OQB65_RS03960
• Genome accession: NZ_CP110808
• Coordinates: 887507..888379 (-)
• Length: 290 a.a.
• NCBI ID: WP_024638544.1
• Uniprot ID: -
• Organism: Pseudomonas syringae strain MUP20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 882507..893379

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OQB65_RS03930 (OQB65_03930)	-	883294..884592 (-)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-
OQB65_RS03935 (OQB65_03935)	-	884809..885342 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
OQB65_RS03940 (OQB65_03940)	-	885339..885968 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
OQB65_RS03945 (OQB65_03945)	-	886094..886555 (+)	462	WP_003345128.1	hypothetical protein	-
OQB65_RS03950 (OQB65_03950)	yacG	886681..886890 (-)	210	WP_024960618.1	DNA gyrase inhibitor YacG	-
OQB65_RS03955 (OQB65_03955)	coaE	886887..887510 (-)	624	WP_266001758.1	dephospho-CoA kinase	-
OQB65_RS03960 (OQB65_03960)	pilD	887507..888379 (-)	873	WP_024638544.1	prepilin peptidase	Machinery gene
OQB65_RS03965 (OQB65_03965)	pilC	888381..889598 (-)	1218	WP_100073388.1	type II secretion system F family protein	Machinery gene
OQB65_RS03970 (OQB65_03970)	pilB	889601..891295 (-)	1695	WP_103694577.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OQB65_RS03975 (OQB65_03975)	pilA2	891522..891953 (+)	432	WP_103694589.1	pilin	Machinery gene
OQB65_RS26395	-	892214..892428 (-)	215	Protein_784	winged helix-turn-helix transcriptional regulator	-
OQB65_RS03980 (OQB65_03980)	-	892638..893330 (+)	693	WP_338129179.1	DUF433 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32021.24 Da
• Isoelectric Point: 8.3734

>NTDB_id=756279 OQB65_RS03960 WP_024638544.1 887507..888379(-) (pilD) [Pseudomonas syringae strain MUP20]
MPLLDLLASSPLAFVITCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=756279 OQB65_RS03960 WP_024638544.1 887507..888379(-) (pilD) [Pseudomonas syringae strain MUP20]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCCTGGCCTTCGTCATCACCTGCTGCATCCTCGGTCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGTTGCCCGCATTGCGCACACAAAATCCGC
CCGTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTATCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGCGG
CAATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATAGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACCTCGCTGAATGATGCGCTGTGGGGTGCAGT
GGCCGGGTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.169	97.931	0.521
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.19	94.483	0.493
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476