Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OPR71_RS02420 Genome accession   NZ_CP110670
Coordinates   505124..506230 (+) Length   368 a.a.
NCBI ID   WP_206954666.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain AUSMDU00064140     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500124..511230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPR71_RS02385 (OPR71_02385) - 500164..500766 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  OPR71_RS02390 (OPR71_02390) - 500885..501316 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  OPR71_RS02395 (OPR71_02395) yggU 501445..501735 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  OPR71_RS02400 (OPR71_02400) - 501735..502292 (-) 558 WP_005381012.1 YggT family protein -
  OPR71_RS02405 (OPR71_02405) proC 502345..503163 (-) 819 WP_206954662.1 pyrroline-5-carboxylate reductase -
  OPR71_RS02410 (OPR71_02410) - 503323..504024 (-) 702 WP_258460214.1 YggS family pyridoxal phosphate-dependent enzyme -
  OPR71_RS02415 (OPR71_02415) pilT 504053..505093 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  OPR71_RS02420 (OPR71_02420) pilU 505124..506230 (+) 1107 WP_206954666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OPR71_RS02425 (OPR71_02425) ruvX 506292..506717 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  OPR71_RS02430 (OPR71_02430) - 506753..507316 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  OPR71_RS02435 (OPR71_02435) gshB 507445..508395 (-) 951 WP_005381021.1 glutathione synthase -
  OPR71_RS02440 (OPR71_02440) rsmE 508409..509140 (-) 732 WP_005381022.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OPR71_RS02445 (OPR71_02445) endA 509254..509949 (-) 696 WP_025768831.1 deoxyribonuclease I -
  OPR71_RS02450 (OPR71_02450) - 510100..510597 (-) 498 WP_054866486.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41356.49 Da        Isoelectric Point: 6.5948

>NTDB_id=755898 OPR71_RS02420 WP_206954666.1 505124..506230(+) (pilU) [Vibrio alginolyticus strain AUSMDU00064140]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLKSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSENDLRLMLKTQRGEAFSTSSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=755898 OPR71_RS02420 WP_206954666.1 505124..506230(+) (pilU) [Vibrio alginolyticus strain AUSMDU00064140]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGTTCGGGTA
AATCGACCACTATGGCGGCGATGACAGGCTATCGAAACAGTAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAATCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGACTTGTCGATGAATCTGAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAAGAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGAAAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGAGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375