Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONS87_RS18285 Genome accession   NZ_CP110416
Coordinates   3823282..3824418 (-) Length   378 a.a.
NCBI ID   WP_104356386.1    Uniprot ID   A0A2S5T7Z3
Organism   Caldimonas thermodepolymerans strain DSM 15264     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3818282..3829418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONS87_RS18255 (ONS87_18255) - 3818566..3819585 (+) 1020 WP_104356380.1 septal ring lytic transglycosylase RlpA family protein -
  ONS87_RS18260 (ONS87_18260) rsmI 3819589..3820488 (-) 900 WP_132764857.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ONS87_RS18265 (ONS87_18265) - 3820481..3820849 (+) 369 WP_104356382.1 YraN family protein -
  ONS87_RS18270 (ONS87_18270) - 3820977..3821573 (+) 597 WP_132764860.1 phosphoheptose isomerase -
  ONS87_RS18275 (ONS87_18275) - 3821570..3822253 (+) 684 WP_232529351.1 BON domain-containing protein -
  ONS87_RS18280 (ONS87_18280) - 3822321..3823214 (-) 894 WP_104356385.1 NAD(P)-dependent oxidoreductase -
  ONS87_RS18285 (ONS87_18285) pilU 3823282..3824418 (-) 1137 WP_104356386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ONS87_RS18290 (ONS87_18290) - 3824471..3825124 (-) 654 WP_104356387.1 cyclic nucleotide-binding domain-containing protein -
  ONS87_RS18295 (ONS87_18295) pilT 3825164..3826207 (-) 1044 WP_104356388.1 type IV pilus twitching motility protein PilT Machinery gene
  ONS87_RS18300 (ONS87_18300) - 3826282..3826968 (+) 687 WP_132764863.1 YggS family pyridoxal phosphate-dependent enzyme -
  ONS87_RS18305 (ONS87_18305) - 3826984..3827466 (-) 483 WP_104356390.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42075.52 Da        Isoelectric Point: 6.5066

>NTDB_id=754914 ONS87_RS18285 WP_104356386.1 3823282..3824418(-) (pilU) [Caldimonas thermodepolymerans strain DSM 15264]
MERDQASKFINDLLRLMIARNGSDLFLTADFPPAIKVDGRVTKVSPQPLTGQHTMALARSIMNDKQAAEFERTKECNFAI
APQGIGRFRVNAFVQQGQVGLVLRVIPQTLPTIDAMGLPQVLKEIAMTKRGLVIMVGATGSGKSTTLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGIDTDGWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKAMVSQRLLPRQEGKGRIAAVEILLNTPLISELIFKGEVAEIKEIMKKSRELG
MQTFDQALFDLYEGNFITYEDALRNADSVNDLRLQIKLNSQRARNADLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=754914 ONS87_RS18285 WP_104356386.1 3823282..3824418(-) (pilU) [Caldimonas thermodepolymerans strain DSM 15264]
ATGGAACGAGATCAGGCCTCCAAATTCATCAACGACCTGCTGCGGCTGATGATCGCCCGCAACGGCTCGGACCTGTTCCT
GACGGCCGACTTTCCCCCGGCCATCAAGGTCGACGGCCGCGTCACCAAGGTCTCGCCCCAGCCGCTGACCGGGCAGCACA
CGATGGCGCTGGCGCGCTCCATCATGAACGACAAGCAGGCCGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
GCGCCGCAGGGCATCGGGCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCCAGGTCGGCCTGGTGCTGCGGGTGATCCC
GCAGACGCTGCCGACCATCGACGCGATGGGCCTGCCGCAGGTGCTCAAGGAAATCGCGATGACCAAGCGCGGCCTGGTCA
TCATGGTGGGCGCGACGGGCTCGGGCAAGAGCACGACGCTGGCGGCCATGGTCGACTACCGCAACGAGAACTCCTACGGC
CACATCATCACGATCGAGGACCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGCAT
CGACACCGACGGCTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCG
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTCAACCT
CAAGGCCATGGTCTCGCAGCGCCTGCTGCCGCGCCAGGAGGGCAAGGGCCGCATCGCGGCGGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGAGCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAAGGCAACTTCATCACCTACGAGGACGCGTTGCGCAACGCCGA
TTCGGTCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAACGCCGACCTGGCGGCCGGCACCGAGC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(85-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S5T7Z3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.115

96.825

0.611
  pilU Acinetobacter baylyi ADP1

58.496

94.974

0.556
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.368

89.153

0.431
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

44.51

89.153

0.397
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

43.844

88.095

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.795

83.862

0.376
  pilT Vibrio cholerae strain A1552

44.795

83.862

0.376