Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   UKS_RS00990 Genome accession   NZ_AP021887
Coordinates   195284..196225 (+) Length   313 a.a.
NCBI ID   WP_156011420.1    Uniprot ID   -
Organism   Streptococcus sp. 116-D4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 190284..201225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UKS_RS00975 (UKS_01750) nagA 192318..193469 (+) 1152 WP_156011418.1 N-acetylglucosamine-6-phosphate deacetylase -
  UKS_RS00980 (UKS_01760) - 193632..194690 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  UKS_RS00985 (UKS_01770) - 194842..195207 (+) 366 WP_156011419.1 DUF1033 family protein -
  UKS_RS00990 (UKS_01780) comGA/cglA/cilD 195284..196225 (+) 942 WP_156011420.1 competence type IV pilus ATPase ComGA Machinery gene
  UKS_RS00995 (UKS_01790) comGB/cglB 196173..197189 (+) 1017 WP_156013011.1 competence type IV pilus assembly protein ComGB Machinery gene
  UKS_RS01000 (UKS_01800) comGC/cglC 197191..197514 (+) 324 WP_049496909.1 comG operon protein ComGC Machinery gene
  UKS_RS01005 (UKS_01810) comGD/cglD 197477..197911 (+) 435 WP_049496906.1 competence type IV pilus minor pilin ComGD Machinery gene
  UKS_RS01010 (UKS_01820) comGE/cglE 197874..198176 (+) 303 WP_153197443.1 competence type IV pilus minor pilin ComGE Machinery gene
  UKS_RS01015 (UKS_01830) comGF/cglF 198139..198600 (+) 462 WP_049496903.1 competence type IV pilus minor pilin ComGF Machinery gene
  UKS_RS01020 (UKS_01840) comGG/cglG 198578..198991 (+) 414 WP_156011421.1 competence type IV pilus minor pilin ComGG Machinery gene
  UKS_RS01025 (UKS_01850) - 199024..199611 (+) 588 WP_156011422.1 class I SAM-dependent methyltransferase -
  UKS_RS01030 (UKS_01860) comYH 199673..200626 (+) 954 WP_156011423.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35416.40 Da        Isoelectric Point: 6.6619

>NTDB_id=75320 UKS_RS00990 WP_156011420.1 195284..196225(+) (comGA/cglA/cilD) [Streptococcus sp. 116-D4]
MVQEIAQEIIRSARKKGAQDIYFVPKFDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDKEQDLHFWFQDIGELGQQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSKEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIGQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=75320 UKS_RS00990 WP_156011420.1 195284..196225(+) (comGA/cglA/cilD) [Streptococcus sp. 116-D4]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCCCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
CGATGCCTATGAGCTTCATATGAGGGTGGGAGATGAGCGCTGTAAAATCGGTTCTTATGATTTTGAAAAGTTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTAGCGGGTATGAATGTTGGGGAGAAGAGACGTAGTCAACTTGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCATCTCTACGCTTATCTACCGTGGGTGATTATCGAGGACATGAGAGTTTAGTTATTCGCTTGTT
GCATGATAAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGGAGAACTGGGCCAGCAGTACAGGCAACGTGGTCTCT
ATCTCTTTGCTGGCCCAGTCGGAAGTGGCAAGACGACCCTGATGCACGAATTAGCTAAATCCCTCTTTAAGGGACAGCAA
GTCATGTCCATCGAGGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGCTAAATGAAGCCATCGGACTAAC
CTATGAAAATCTGATAAAACTTTCCTTGCGTCATCGTCCAGACCTCCTGATTATCGGAGAAATTCGAGACAGCGAGACGG
CGCGTGCAGTGGTTAGAGCTAGTTTGACAGGTGCGACAGTATTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAACGCCTGCTGGAGTTGGGTGTGAGTAAGGAGGAATTAGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGGCCAGC
TTCTTAAAGATGGACATATCACAAGCCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.527

100

0.955
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

55.911

100

0.559
  comGA Latilactobacillus sakei subsp. sakei 23K

42.279

86.901

0.367

Multiple sequence alignment