Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   UKS_RS00995 Genome accession   NZ_AP021887
Coordinates   196173..197189 (+) Length   338 a.a.
NCBI ID   WP_156013011.1    Uniprot ID   -
Organism   Streptococcus sp. 116-D4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 191173..202189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UKS_RS00975 (UKS_01750) nagA 192318..193469 (+) 1152 WP_156011418.1 N-acetylglucosamine-6-phosphate deacetylase -
  UKS_RS00980 (UKS_01760) - 193632..194690 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  UKS_RS00985 (UKS_01770) - 194842..195207 (+) 366 WP_156011419.1 DUF1033 family protein -
  UKS_RS00990 (UKS_01780) comGA/cglA/cilD 195284..196225 (+) 942 WP_156011420.1 competence type IV pilus ATPase ComGA Machinery gene
  UKS_RS00995 (UKS_01790) comGB/cglB 196173..197189 (+) 1017 WP_156013011.1 competence type IV pilus assembly protein ComGB Machinery gene
  UKS_RS01000 (UKS_01800) comGC/cglC 197191..197514 (+) 324 WP_049496909.1 comG operon protein ComGC Machinery gene
  UKS_RS01005 (UKS_01810) comGD/cglD 197477..197911 (+) 435 WP_049496906.1 competence type IV pilus minor pilin ComGD Machinery gene
  UKS_RS01010 (UKS_01820) comGE/cglE 197874..198176 (+) 303 WP_153197443.1 competence type IV pilus minor pilin ComGE Machinery gene
  UKS_RS01015 (UKS_01830) comGF/cglF 198139..198600 (+) 462 WP_049496903.1 competence type IV pilus minor pilin ComGF Machinery gene
  UKS_RS01020 (UKS_01840) comGG/cglG 198578..198991 (+) 414 WP_156011421.1 competence type IV pilus minor pilin ComGG Machinery gene
  UKS_RS01025 (UKS_01850) - 199024..199611 (+) 588 WP_156011422.1 class I SAM-dependent methyltransferase -
  UKS_RS01030 (UKS_01860) comYH 199673..200626 (+) 954 WP_156011423.1 class I SAM-dependent methyltransferase Machinery gene
  UKS_RS01035 (UKS_01870) - 200676..201866 (+) 1191 WP_156011424.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38529.52 Da        Isoelectric Point: 9.7256

>NTDB_id=75321 UKS_RS00995 WP_156013011.1 196173..197189(+) (comGB/cglB) [Streptococcus sp. 116-D4]
MDISQAFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSGNIATQIIGNLPQIF
LGMLGFVSILALLALTFYKRSSKMRVFSILARLPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLARALQNGREFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=75321 UKS_RS00995 WP_156013011.1 196173..197189(+) (comGB/cglB) [Streptococcus sp. 116-D4]
ATGGACATATCACAAGCCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATTACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCACCTGGTAGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAATGTG
TGACCCAGATGCGCACGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGCGCTATT
GTCACTCAACTATCTCTAGCTGAAGTTCATGGGAATCTTCATCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
AGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGCAATTATCTACTACCACAACTGGATAGTGGCAATATTGCTACCCAAATCATTGGCAATCTGCCACAAATTTTT
CTAGGCATGCTAGGGTTTGTTTCCATACTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCCAAGATGCGCGTCTT
TTCTATCTTAGCACGCCTTCCCTTCTTTGGAATTTTTGTGCAGACCTATCTGACAGCCTATTATGCGCGTGAATGGGGCA
ATATGATTTCACAGGGAATGGAGCTGACACAGATTTTTCAGATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCGAGCCCTGCAAAATGGTCGAGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTTATTATCGAGTATGGGGAAGTTAAGTCCAAGCTTGGGAGTGAATTGGAGATTTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAACTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae R6

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae TIGR4

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae Rx1

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae D39

95.858

100

0.959
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus mitis NCTC 12261

95.562

100

0.956
  comYB Streptococcus gordonii str. Challis substr. CH1

72.024

99.408

0.716
  comYB Streptococcus mutans UA140

58.544

93.491

0.547
  comYB Streptococcus mutans UA159

58.544

93.491

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment