Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   OHM81_RS29540 Genome accession   NZ_CP109857
Coordinates   6425955..6426389 (+) Length   144 a.a.
NCBI ID   WP_338498913.1    Uniprot ID   -
Organism   Delftia tsuruhatensis strain GSK TC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6426574..6441311 6425955..6426389 flank 185


Gene organization within MGE regions


Location: 6425955..6441311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHM81_RS29540 pilE 6425955..6426389 (+) 435 WP_338498913.1 pilin Machinery gene
  OHM81_RS29545 pilE 6426485..6426907 (+) 423 WP_338498916.1 pilin Machinery gene
  OHM81_RS29550 - 6426952..6428985 (+) 2034 WP_338498919.1 hypothetical protein -
  OHM81_RS29555 - 6428982..6429863 (+) 882 WP_338498922.1 glycosyltransferase family 2 protein -
  OHM81_RS29560 - 6429901..6430950 (+) 1050 WP_338498925.1 glycosyltransferase family 2 protein -
  OHM81_RS29565 - 6430952..6432436 (-) 1485 WP_338498928.1 oligosaccharide flippase family protein -
  OHM81_RS29570 - 6432454..6433134 (-) 681 WP_338498930.1 cephalosporin hydroxylase family protein -
  OHM81_RS29575 - 6433174..6434337 (-) 1164 WP_338498932.1 class I SAM-dependent methyltransferase -
  OHM81_RS29580 - 6434330..6435220 (-) 891 WP_338498935.1 NAD(P)-dependent oxidoreductase -
  OHM81_RS29585 - 6435217..6436461 (-) 1245 WP_338500895.1 class I SAM-dependent methyltransferase -
  OHM81_RS29590 rfbC 6436464..6437030 (-) 567 WP_338498938.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  OHM81_RS29595 rfbG 6437027..6438118 (-) 1092 WP_338498941.1 CDP-glucose 4,6-dehydratase -
  OHM81_RS29600 rfbF 6438100..6438870 (-) 771 WP_338498944.1 glucose-1-phosphate cytidylyltransferase -
  OHM81_RS29605 - 6438884..6439900 (-) 1017 WP_338498947.1 glycosyltransferase family 4 protein -
  OHM81_RS29610 - 6440121..6441302 (-) 1182 WP_338498950.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14960.19 Da        Isoelectric Point: 10.1965

>NTDB_id=750239 OHM81_RS29540 WP_338498913.1 6425955..6426389(+) (pilE) [Delftia tsuruhatensis strain GSK TC1]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTKRARVSEALSLMSGAKASVTEYYSTNNTWPSNNASAGLAPATSI
NGGSVLSVTVTGSKITAVVRTNIESGSKKVVLKGTATSGGVTWSCKNAISGTDVNAKFLPSECR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=750239 OHM81_RS29540 WP_338498913.1 6425955..6426389(+) (pilE) [Delftia tsuruhatensis strain GSK TC1]
ATGAAGCGTTCCATCCAGCAAGGTTTTACCTTGATCGAATTGATGATCGTTGTGGCGATCATCGGCATCTTGGCAGCGGT
TGCGCTGCCGGCTTATCAGGATTACACGAAGCGTGCCCGCGTTTCGGAAGCCCTGTCGCTGATGAGCGGCGCGAAGGCCT
CGGTGACCGAGTACTATTCTACAAACAACACATGGCCTAGCAACAATGCGTCTGCAGGCTTGGCCCCCGCAACCTCCATC
AATGGCGGTAGCGTGCTTAGCGTCACTGTGACAGGTAGCAAGATCACTGCCGTGGTTCGGACCAATATCGAATCGGGCAG
CAAGAAGGTGGTCCTCAAGGGTACGGCAACGTCTGGCGGAGTGACTTGGAGCTGCAAGAACGCCATTTCCGGCACCGATG
TGAACGCCAAGTTCCTGCCAAGCGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

49.045

100

0.535
  pilE Neisseria gonorrhoeae strain FA1090

46.795

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

40.964

100

0.472
  comP Acinetobacter baylyi ADP1

41.026

100

0.444
  pilA2 Legionella pneumophila str. Paris

45.714

97.222

0.444
  pilA2 Legionella pneumophila strain ERS1305867

45

97.222

0.438
  pilA Haemophilus influenzae Rd KW20

42.361

100

0.424
  pilA Haemophilus influenzae 86-028NP

40.972

100

0.41
  pilA Vibrio campbellii strain DS40M4

39.31

100

0.396
  pilA Acinetobacter baumannii strain A118

38.732

98.611

0.382
  pilA/pilA1 Eikenella corrodens VA1

35.256

100

0.382
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.735

100

0.375
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

100

0.375