Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   OHM81_RS29545 Genome accession   NZ_CP109857
Coordinates   6426485..6426907 (+) Length   140 a.a.
NCBI ID   WP_338498916.1    Uniprot ID   -
Organism   Delftia tsuruhatensis strain GSK TC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6426574..6441311 6426485..6426907 flank -333


Gene organization within MGE regions


Location: 6426485..6441311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHM81_RS29545 pilE 6426485..6426907 (+) 423 WP_338498916.1 pilin Machinery gene
  OHM81_RS29550 - 6426952..6428985 (+) 2034 WP_338498919.1 hypothetical protein -
  OHM81_RS29555 - 6428982..6429863 (+) 882 WP_338498922.1 glycosyltransferase family 2 protein -
  OHM81_RS29560 - 6429901..6430950 (+) 1050 WP_338498925.1 glycosyltransferase family 2 protein -
  OHM81_RS29565 - 6430952..6432436 (-) 1485 WP_338498928.1 oligosaccharide flippase family protein -
  OHM81_RS29570 - 6432454..6433134 (-) 681 WP_338498930.1 cephalosporin hydroxylase family protein -
  OHM81_RS29575 - 6433174..6434337 (-) 1164 WP_338498932.1 class I SAM-dependent methyltransferase -
  OHM81_RS29580 - 6434330..6435220 (-) 891 WP_338498935.1 NAD(P)-dependent oxidoreductase -
  OHM81_RS29585 - 6435217..6436461 (-) 1245 WP_338500895.1 class I SAM-dependent methyltransferase -
  OHM81_RS29590 rfbC 6436464..6437030 (-) 567 WP_338498938.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  OHM81_RS29595 rfbG 6437027..6438118 (-) 1092 WP_338498941.1 CDP-glucose 4,6-dehydratase -
  OHM81_RS29600 rfbF 6438100..6438870 (-) 771 WP_338498944.1 glucose-1-phosphate cytidylyltransferase -
  OHM81_RS29605 - 6438884..6439900 (-) 1017 WP_338498947.1 glycosyltransferase family 4 protein -
  OHM81_RS29610 - 6440121..6441302 (-) 1182 WP_338498950.1 glycosyltransferase family 4 protein -

Sequence


Protein


Download         Length: 140 a.a.        Molecular weight: 14819.15 Da        Isoelectric Point: 10.0548

>NTDB_id=750240 OHM81_RS29545 WP_338498916.1 6426485..6426907(+) (pilE) [Delftia tsuruhatensis strain GSK TC1]
MAKGFTLIELMIVLAIFGVLAAIALPNYQNYASRARVTEALNLMAGAKISVTEYRLSRNSWPSNNTDVGLAQPTSIKGSS
VQSVQVNGSVITAIMRSLAVPGGGVVVLKGSNTGDSMTWRCNPSVGTTIQTIFLPSECRQ

Nucleotide


Download         Length: 423 bp        

>NTDB_id=750240 OHM81_RS29545 WP_338498916.1 6426485..6426907(+) (pilE) [Delftia tsuruhatensis strain GSK TC1]
ATGGCCAAAGGTTTCACATTGATAGAGCTAATGATTGTTTTGGCAATCTTTGGCGTACTGGCAGCCATTGCGCTGCCAAA
TTATCAGAACTATGCCTCTCGCGCCAGGGTGACAGAGGCGCTCAACCTGATGGCCGGCGCAAAAATATCGGTAACAGAAT
ACCGGCTCAGCCGAAATTCATGGCCCTCCAATAATACAGACGTGGGTTTGGCCCAACCCACCTCCATAAAAGGCTCCAGC
GTCCAGTCAGTACAGGTCAATGGCAGCGTGATCACGGCGATCATGCGCAGCCTTGCGGTTCCTGGCGGAGGAGTGGTTGT
TTTGAAGGGGAGCAACACGGGAGACTCAATGACCTGGCGATGCAACCCCTCTGTTGGAACGACCATTCAGACAATATTTC
TTCCATCAGAGTGCCGTCAATAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

45.86

100

0.514

  pilE Neisseria gonorrhoeae strain FA1090

43.59

100

0.486

  pilA Ralstonia pseudosolanacearum GMI1000

36.42

100

0.421

  comP Acinetobacter baylyi ADP1

37.662

100

0.414

  pilA2 Legionella pneumophila str. Paris

39.416

97.857

0.386

  pilA2 Legionella pneumophila strain ERS1305867

39.416

97.857

0.386

  pilA/pilA1 Eikenella corrodens VA1

34.868

100

0.379