Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UABAM_RS03300 Genome accession   NZ_AP019860
Coordinates   960910..962001 (+) Length   363 a.a.
NCBI ID   WP_151966576.1    Uniprot ID   A0A5S9F1E3
Organism   Candidatus Uabimicrobium amorphum strain SRT547     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955910..967001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UABAM_RS03280 (UABAM_00667) - 956346..957740 (-) 1395 WP_151966572.1 DUF6134 family protein -
  UABAM_RS03285 (UABAM_00668) - 957743..958147 (-) 405 WP_151966573.1 hypothetical protein -
  UABAM_RS03290 (UABAM_00669) - 958248..959825 (-) 1578 WP_173013108.1 PleD family two-component system response regulator -
  UABAM_RS03295 (UABAM_00670) - 959953..960312 (-) 360 WP_151966575.1 hypothetical protein -
  UABAM_RS03300 (UABAM_00671) pilT 960910..962001 (+) 1092 WP_151966576.1 type IV pilus twitching motility protein PilT Machinery gene
  UABAM_RS03305 (UABAM_00672) - 962085..963443 (+) 1359 WP_151966577.1 type IV pilus twitching motility protein PilT -
  UABAM_RS03310 (UABAM_00673) - 963574..964032 (+) 459 WP_151966578.1 hypothetical protein -
  UABAM_RS03315 (UABAM_00674) - 964037..964594 (+) 558 WP_151966579.1 hypothetical protein -
  UABAM_RS03320 (UABAM_00675) - 964610..965572 (+) 963 WP_151966580.1 hypothetical protein -
  UABAM_RS03325 (UABAM_00676) nth 965541..966155 (+) 615 WP_151966581.1 endonuclease III -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 41159.30 Da        Isoelectric Point: 6.7246

>NTDB_id=74488 UABAM_RS03300 WP_151966576.1 960910..962001(+) (pilT) [Candidatus Uabimicrobium amorphum strain SRT547]
MAKIDAFFRLMTVQGASDLHLSSGNPPALRIRGDLERVKFKQLEDGELKELLYEICPEHKAKVFEETGDIDFAYELPNVG
RYRANYFQQAHGVSAVFRQIPTEILTVEQLGLPEVVKRFATYHKGLILVTGPTGSGKSTTLAAIIDYANKTRKDHILTVE
DPVEFKHNSYNCIVNHREVGKDTKSFSAALRGALREDPDIILVGEMRDQETIQLAIEAAATGHLVFGTLHTQNATKTVDR
IIEVFPANMQDQIRTTLSESLKAVVSQTLFKRKDGSGRICALEILIQTPAVSALVREGKTHQLINVIQTNRRMGMRMLDD
HIEELLDENLIREDEAFDKCIEKKRFIKYLKEIPQEYKDMFKM

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=74488 UABAM_RS03300 WP_151966576.1 960910..962001(+) (pilT) [Candidatus Uabimicrobium amorphum strain SRT547]
ATGGCAAAAATAGATGCTTTTTTTCGATTGATGACAGTCCAAGGAGCTTCCGATCTACATTTATCCTCTGGTAATCCTCC
AGCTCTACGTATTCGTGGAGATTTAGAAAGAGTAAAGTTTAAACAGCTAGAAGATGGTGAGTTAAAAGAACTTCTTTACG
AAATATGCCCAGAGCACAAGGCAAAAGTATTCGAAGAAACAGGTGATATCGACTTTGCTTATGAGCTACCTAATGTGGGG
CGATATCGGGCAAACTACTTTCAACAAGCTCATGGGGTTTCGGCTGTTTTTAGACAAATTCCGACAGAAATACTCACTGT
AGAGCAACTTGGTTTACCCGAAGTGGTAAAACGTTTTGCCACTTATCACAAAGGTTTGATTCTCGTAACAGGTCCGACAG
GTAGTGGAAAATCTACCACCCTGGCCGCGATTATTGACTATGCAAATAAAACGCGGAAAGATCATATCTTGACGGTAGAA
GACCCCGTAGAATTCAAACACAATTCGTACAATTGTATTGTAAACCACCGCGAAGTGGGAAAAGATACAAAAAGTTTTTC
AGCGGCACTTCGAGGTGCACTGCGCGAAGACCCTGATATTATACTCGTGGGGGAAATGCGTGATCAGGAAACCATTCAGC
TCGCTATCGAAGCGGCGGCAACAGGGCACTTGGTATTCGGAACTCTACATACGCAAAACGCAACAAAAACTGTGGACCGT
ATTATCGAAGTTTTTCCTGCAAACATGCAAGATCAGATACGTACTACTTTGTCTGAATCACTCAAAGCCGTTGTTTCACA
AACGCTTTTTAAGAGAAAAGACGGATCAGGTCGTATTTGCGCCTTAGAAATACTCATACAAACCCCGGCCGTTTCTGCTC
TCGTTCGCGAAGGAAAAACTCACCAACTCATAAACGTTATCCAAACGAATAGACGTATGGGAATGCGTATGCTTGATGAT
CATATTGAAGAACTACTGGATGAAAATCTCATTAGAGAAGACGAAGCTTTTGATAAGTGTATAGAAAAGAAACGTTTTAT
TAAATATCTAAAAGAGATTCCCCAAGAGTACAAAGATATGTTCAAGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S9F1E3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

54.354

91.736

0.499
  pilT Legionella pneumophila strain Lp02

54.381

91.185

0.496
  pilT Legionella pneumophila strain ERS1305867

54.381

91.185

0.496
  pilT Pseudomonas aeruginosa PAK

55.046

90.083

0.496
  pilT Acinetobacter nosocomialis M2

52.695

92.011

0.485
  pilT Acinetobacter baumannii D1279779

52.695

92.011

0.485
  pilT Acinetobacter baumannii strain A118

52.695

92.011

0.485
  pilT Acinetobacter baylyi ADP1

55.063

87.052

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.147

89.807

0.468
  pilT Vibrio cholerae strain A1552

52.147

89.807

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

95.041

0.457
  pilT Neisseria meningitidis 8013

50.779

88.43

0.449
  pilT Neisseria gonorrhoeae MS11

50.467

88.43

0.446
  pilU Pseudomonas stutzeri DSM 10701

38.827

98.623

0.383

Multiple sequence alignment