Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N9L84_RS05330 Genome accession   NZ_CP106785
Coordinates   1122837..1123262 (-) Length   141 a.a.
NCBI ID   WP_263152775.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain G5.110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1117837..1128262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N9L84_RS05300 (N9L84_05300) - 1117908..1118123 (+) 216 WP_213628504.1 hypothetical protein -
  N9L84_RS05305 (N9L84_05305) yacG 1118127..1118327 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  N9L84_RS05310 (N9L84_05310) coaE 1118324..1118935 (-) 612 WP_263152772.1 dephospho-CoA kinase -
  N9L84_RS05315 (N9L84_05315) pilD 1118935..1119804 (-) 870 WP_173173305.1 A24 family peptidase Machinery gene
  N9L84_RS05320 (N9L84_05320) pilC 1119805..1121025 (-) 1221 WP_263152773.1 type II secretion system F family protein Machinery gene
  N9L84_RS05325 (N9L84_05325) pilB 1121029..1122732 (-) 1704 WP_263152774.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N9L84_RS05330 (N9L84_05330) pilA 1122837..1123262 (-) 426 WP_263152775.1 pilin Machinery gene
  N9L84_RS05335 (N9L84_05335) - 1123416..1125128 (+) 1713 WP_263152777.1 PglL family O-oligosaccharyltransferase -
  N9L84_RS05345 (N9L84_05345) nadC 1125311..1126159 (-) 849 WP_263152778.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14338.49 Da        Isoelectric Point: 9.2405

>NTDB_id=735112 N9L84_RS05330 WP_263152775.1 1122837..1123262(-) (pilA) [Pseudomonas tohonis strain G5.110]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYRNYIARSEANTGLQAIAPLKTAVEDGFARGLAGASVSSLGLLGTSVSASP
LGSIAANFADAGTGTLTFTFNGQASPVLRTPAAIHTLTRAADGSWTCTSTAPVDFRPRGCN

Nucleotide


Download         Length: 426 bp        

>NTDB_id=735112 N9L84_RS05330 WP_263152775.1 1122837..1123262(-) (pilA) [Pseudomonas tohonis strain G5.110]
ATGAAAGCTCAAAAAGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGGCATCCTGGCTGCAATTGCCAT
CCCGGCTTACCGCAATTACATCGCCCGTTCTGAAGCCAACACTGGCCTGCAAGCTATCGCCCCGCTGAAAACTGCGGTTG
AAGATGGTTTCGCACGCGGCCTCGCTGGTGCCTCTGTTTCCAGCCTGGGCCTTCTTGGCACCAGCGTAAGCGCCAGCCCC
CTGGGCAGTATCGCGGCGAACTTTGCAGATGCAGGCACTGGTACTCTGACCTTCACCTTCAACGGCCAGGCAAGCCCGGT
TCTGCGGACCCCGGCAGCCATCCATACCCTTACTCGTGCGGCAGATGGCTCCTGGACCTGCACCTCCACTGCTCCTGTTG
ATTTCCGTCCTCGCGGCTGCAACTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

51.974

100

0.56
  pilA Acinetobacter baumannii strain A118

48.571

99.291

0.482
  pilA Vibrio parahaemolyticus RIMD 2210633

48.872

94.326

0.461
  pilA Vibrio cholerae C6706

41.333

100

0.44
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.333

100

0.44
  pilA Vibrio cholerae strain A1552

41.333

100

0.44
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

100

0.426
  pilA2 Legionella pneumophila str. Paris

42.857

99.291

0.426
  pilA2 Legionella pneumophila strain ERS1305867

42.857

99.291

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.754

97.872

0.418
  comP Acinetobacter baylyi ADP1

38.667

100

0.411