Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N9L84_RS05320 Genome accession   NZ_CP106785
Coordinates   1119805..1121025 (-) Length   406 a.a.
NCBI ID   WP_263152773.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain G5.110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1114805..1126025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N9L84_RS05275 (N9L84_05275) - 1115229..1115402 (-) 174 WP_173173318.1 DUF3094 domain-containing protein -
  N9L84_RS05280 (N9L84_05280) - 1115489..1116115 (-) 627 WP_173173316.1 DUF1780 domain-containing protein -
  N9L84_RS05285 (N9L84_05285) - 1116228..1116653 (+) 426 WP_263152770.1 GNAT family N-acetyltransferase -
  N9L84_RS05290 (N9L84_05290) - 1116650..1117117 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  N9L84_RS05295 (N9L84_05295) - 1117114..1117800 (+) 687 WP_263152771.1 energy-coupling factor ABC transporter permease -
  N9L84_RS05300 (N9L84_05300) - 1117908..1118123 (+) 216 WP_213628504.1 hypothetical protein -
  N9L84_RS05305 (N9L84_05305) yacG 1118127..1118327 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  N9L84_RS05310 (N9L84_05310) coaE 1118324..1118935 (-) 612 WP_263152772.1 dephospho-CoA kinase -
  N9L84_RS05315 (N9L84_05315) pilD 1118935..1119804 (-) 870 WP_173173305.1 A24 family peptidase Machinery gene
  N9L84_RS05320 (N9L84_05320) pilC 1119805..1121025 (-) 1221 WP_263152773.1 type II secretion system F family protein Machinery gene
  N9L84_RS05325 (N9L84_05325) pilB 1121029..1122732 (-) 1704 WP_263152774.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N9L84_RS05330 (N9L84_05330) pilA 1122837..1123262 (-) 426 WP_263152775.1 pilin Machinery gene
  N9L84_RS05335 (N9L84_05335) - 1123416..1125128 (+) 1713 WP_263152777.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44040.68 Da        Isoelectric Point: 9.8996

>NTDB_id=735110 N9L84_RS05320 WP_263152773.1 1119805..1121025(-) (pilC) [Pseudomonas tohonis strain G5.110]
MAEKALKASVFKWEGTDKKGSKVSGEISGLNPALVKAQLRKQGVNPTKVRKKAASLFGAGKKIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNQNMRKLVDEVKQEVASGNSLANSLRKKPLFFDDLYCNLVDSGEQAGALENLLDRIATYKEKT
ESLKAKIKKAMTYPIAVVLVALTVSAILLIKVVPQFQAIFSGFGAELPAFTQMVINLSEVLQTWWFVFLIAIFGASFTLR
EAHRRSEKFRDAMDRGVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVAKVKQDVSTGM
QLNFSMRTTGVFPSMAVQMTAIGEESGALDTMLDKVASFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=735110 N9L84_RS05320 WP_263152773.1 1119805..1121025(-) (pilC) [Pseudomonas tohonis strain G5.110]
ATGGCGGAAAAAGCACTAAAAGCGAGTGTCTTCAAATGGGAAGGCACCGACAAAAAAGGCAGCAAGGTCAGCGGTGAAAT
CAGCGGGCTAAATCCTGCGCTGGTCAAAGCACAACTGCGCAAGCAAGGGGTCAACCCGACAAAGGTACGCAAGAAAGCTG
CTTCCCTTTTCGGTGCGGGCAAGAAGATCAAGCCTTTGGACATTGCGCTCTTCACTCGGCAAATGGCCACCATGATGAAG
GCAGGTGTTCCTCTCCTTCAAAGCTTTGACATCATCGGGGAAGGATTCGACAACCAGAACATGCGCAAACTGGTTGACGA
AGTTAAGCAAGAGGTGGCCTCAGGCAACAGTCTCGCCAACTCACTTCGTAAGAAGCCCCTCTTCTTTGATGACCTGTATT
GCAACCTGGTGGACTCAGGCGAGCAGGCCGGTGCACTCGAAAACCTCCTTGATCGTATTGCCACCTATAAGGAAAAGACC
GAATCCCTGAAGGCCAAGATCAAGAAGGCGATGACGTACCCCATCGCAGTCGTCCTAGTGGCACTGACTGTGTCGGCCAT
CCTATTGATCAAGGTCGTCCCCCAGTTCCAAGCGATATTCTCGGGCTTTGGAGCAGAGCTGCCTGCCTTTACTCAGATGG
TGATCAACCTCTCTGAGGTTTTGCAAACCTGGTGGTTCGTCTTCCTGATCGCTATTTTTGGTGCCTCCTTCACGTTGCGT
GAAGCACATCGGCGCTCCGAGAAGTTCCGCGACGCCATGGACCGTGGGGTCCTGAAGCTGCCGATCGTGGGTGACATCCT
CTACAAATCTGCTGTTGCCCGGTTCGCACGCACCCTGTCTACTACCTTTGCTGCCGGTGTTCCGCTCGTGGAAGCACTGG
ACTCGGTATCTGGCGCCACGGGCAACGTGGTCTTCAAGAATGCGGTAGCCAAGGTCAAACAGGACGTGTCGACAGGTATG
CAATTGAACTTCTCAATGCGTACTACTGGTGTGTTCCCGTCAATGGCAGTTCAGATGACAGCTATCGGTGAGGAATCCGG
CGCACTGGACACCATGCTCGACAAGGTCGCCTCCTTCTACGAAGAAGAAGTCGACAACGCCGTCGACAACCTGACCACCC
TGATGGAGCCCATGATCATGGCTGTCCTCGGCGTGCTGGTCGGCGGCCTGATCATCGCGATGTACCTGCCTATCTTCCAG
CTCGGTTCGGTAGTGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

99.754

0.793
  pilC Acinetobacter baylyi ADP1

58.621

100

0.586
  pilC Acinetobacter baumannii D1279779

58.765

99.754

0.586
  pilC Legionella pneumophila strain ERS1305867

54.04

97.537

0.527
  pilC Vibrio cholerae strain A1552

42.857

100

0.429
  pilG Neisseria meningitidis 44/76-A

42.786

99.015

0.424
  pilG Neisseria gonorrhoeae MS11

42.786

99.015

0.424
  pilC Vibrio campbellii strain DS40M4

42.317

97.783

0.414
  pilC Thermus thermophilus HB27

37.75

98.522

0.372