Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N9L84_RS05325 Genome accession   NZ_CP106785
Coordinates   1121029..1122732 (-) Length   567 a.a.
NCBI ID   WP_263152774.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain G5.110     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1116029..1127732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N9L84_RS05285 (N9L84_05285) - 1116228..1116653 (+) 426 WP_263152770.1 GNAT family N-acetyltransferase -
  N9L84_RS05290 (N9L84_05290) - 1116650..1117117 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  N9L84_RS05295 (N9L84_05295) - 1117114..1117800 (+) 687 WP_263152771.1 energy-coupling factor ABC transporter permease -
  N9L84_RS05300 (N9L84_05300) - 1117908..1118123 (+) 216 WP_213628504.1 hypothetical protein -
  N9L84_RS05305 (N9L84_05305) yacG 1118127..1118327 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  N9L84_RS05310 (N9L84_05310) coaE 1118324..1118935 (-) 612 WP_263152772.1 dephospho-CoA kinase -
  N9L84_RS05315 (N9L84_05315) pilD 1118935..1119804 (-) 870 WP_173173305.1 A24 family peptidase Machinery gene
  N9L84_RS05320 (N9L84_05320) pilC 1119805..1121025 (-) 1221 WP_263152773.1 type II secretion system F family protein Machinery gene
  N9L84_RS05325 (N9L84_05325) pilB 1121029..1122732 (-) 1704 WP_263152774.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N9L84_RS05330 (N9L84_05330) pilA 1122837..1123262 (-) 426 WP_263152775.1 pilin Machinery gene
  N9L84_RS05335 (N9L84_05335) - 1123416..1125128 (+) 1713 WP_263152777.1 PglL family O-oligosaccharyltransferase -
  N9L84_RS05345 (N9L84_05345) nadC 1125311..1126159 (-) 849 WP_263152778.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62487.57 Da        Isoelectric Point: 5.4869

>NTDB_id=735111 N9L84_RS05325 WP_263152774.1 1121029..1122732(-) (pilB) [Pseudomonas tohonis strain G5.110]
MSDSISLTGLARQIVIAELLDEKSAQQAQQQALRNRMSLVTYIVQNKLVKSRPLAEIAAEQFGVAFFDLSAIDKDSQPKE
LISEKLIRQHRIIPLWRRGNKLFIGVSDPTNHQAITDVQFSTGLTTEAILVEDDKIGEAIERFFESSNSGMDDLGDVDLD
GVDIESVDEDKDDKSGGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNSLPTLWGEKIVMRILDPSSAQMGIDALGYEEVQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSSCKKEVDVPRETLLKEGFP
ESKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTTALQRIIMEEGNSIDIAAQMRKDGFNDLRASGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=735111 N9L84_RS05325 WP_263152774.1 1121029..1122732(-) (pilB) [Pseudomonas tohonis strain G5.110]
ATGAGCGACTCAATTTCCCTTACAGGTCTGGCACGCCAGATCGTCATCGCCGAACTGCTTGACGAGAAAAGTGCTCAGCA
AGCGCAGCAGCAGGCGCTGCGCAACAGGATGTCGCTGGTGACCTATATCGTTCAGAACAAGCTGGTAAAGAGCCGCCCTC
TAGCGGAAATCGCCGCGGAACAGTTCGGAGTCGCGTTTTTTGACCTTTCAGCAATAGACAAAGACTCCCAGCCCAAGGAG
CTCATCAGCGAGAAATTGATTCGGCAACACCGGATCATCCCGCTCTGGAGACGGGGCAACAAGCTCTTCATCGGCGTATC
CGACCCAACTAATCACCAAGCCATCACAGATGTACAGTTCAGTACTGGACTTACAACTGAAGCAATTCTGGTCGAGGACG
ACAAAATCGGTGAGGCTATAGAGCGCTTCTTCGAATCTTCGAACAGCGGTATGGATGATCTGGGGGACGTCGACCTAGAC
GGTGTCGACATAGAAAGTGTTGACGAGGACAAGGACGACAAGAGTGGAGGCGGGGATGCAGACGATGCTCCGGTTGTTCG
CTTCGTCAACAAAATGTTGCTAGACGCAATCAAAGGAGGCTCTTCCGACCTTCATTTCGAGCCCTATGAGAAGAGCTATC
GCGTCCGTCTTCGTACTGACGGCATTCTTCACGAAGTGGCACGCCCTCCAATCCAACTGGCCAGCAGAATTTCTGCCCGC
TTAAAGGTCATGGCCGCACTTGACATATCCGAGCGCCGCAAACCGCAGGACGGTCGGATAAAGATGAAAATCTCCAAGAC
TAAGGCCATCGACTTCCGCGTCAATAGCCTCCCGACACTATGGGGCGAGAAGATCGTAATGCGGATTCTCGACCCTTCCA
GTGCCCAGATGGGTATCGATGCCCTTGGGTACGAGGAGGTGCAGAAAGAACTCTATCTGAGCGCCCTGAAACAACCTCAA
GGCATGATCCTGGTAACAGGCCCTACGGGCTCGGGCAAGACAGTTTCGCTCTATACCGGCCTCAACATCCTCAATACGGT
CGATATCAACATTTCCACGGCAGAAGATCCCGTAGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AAGGTATGGATTTCGCCCAGGCCCTTCGCGCCTTTCTGCGCCAAGACCCTGATGTGATCATGGTCGGCGAGATTCGTGAC
CTCGAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGTCATATGGTGATGTCCACGCTGCACACCAACAGCGCGGC
TGAAACCTTGACCCGCCTGCGCAACATGGGGGTCCCGGCCTTCAATATCGCAACGTCGGTCAACCTCATCATCGCGCAGC
GCCTGGCCAGGAAGCTCTGCAGCAGTTGTAAGAAGGAGGTAGATGTGCCTCGTGAAACGCTGCTCAAGGAAGGCTTCCCC
GAGAGCAAGATCGGCACCTTCAAGATCTATGGCCCTGTAGGTTGCGAAAACTGCAAGGGCGGTTACAAGGGCAGGGTCGG
TATTTATGAAGTGGTTAAAAACACGACCGCGCTACAACGCATTATCATGGAGGAAGGCAACTCTATTGATATCGCCGCGC
AAATGCGTAAAGACGGCTTCAATGACCTGCGCGCCTCGGGCCTGCTGAAAGCCATGCAAGGCGTAACCAGTCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.019

100

0.594
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.552
  pilB Vibrio cholerae strain A1552

50.179

98.765

0.496
  pilB Vibrio campbellii strain DS40M4

48.85

99.647

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

51.311

94.18

0.483
  pilF Neisseria gonorrhoeae MS11

47.602

99.295

0.473
  pilF Thermus thermophilus HB27

37.279

99.824

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.298

0.369