NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	PALA13_RS05070	Genome accession	NZ_CP106682
Coordinates	1073564..1074016 (+)	Length	150 a.a.
NCBI ID	WP_024947914.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain PALA13
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1068564..1079016

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA13_RS05045 (PALA13_00997)	yacG	1068730..1068930 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PALA13_RS05050 (PALA13_00998)	coaE	1068927..1069538 (-)	612	WP_003112838.1	dephospho-CoA kinase	-
PALA13_RS05055 (PALA13_00999)	pilD	1069535..1070407 (-)	873	WP_023087804.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PALA13_RS05060 (PALA13_01000)	pilC	1070411..1071631 (-)	1221	WP_016253888.1	type 4a pilus biogenesis protein PilC	Machinery gene
PALA13_RS05065 (PALA13_01001)	pilB	1071635..1073335 (-)	1701	WP_009875880.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PALA13_RS05070	pilA	1073564..1074016 (+)	453	WP_024947914.1	pilin	Machinery gene
PALA13_RS05080 (PALA13_01003)	nadC	1074204..1075052 (-)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PALA13_RS05085 (PALA13_01004)	-	1075338..1078007 (+)	2670	WP_269973670.1	DUF1631 family protein	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15622.02 Da Isoelectric Point: 8.4827

                                >NTDB_id=734162 PALA13_RS05070 WP_024947914.1 1073564..1074016(+) (pilA) [Pseudomonas aeruginosa strain PALA13]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG

VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide

Download Length: 453 bp

                                >NTDB_id=734162 PALA13_RS05070 WP_024947914.1 1073564..1074016(+) (pilA) [Pseudomonas aeruginosa strain PALA13]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT

TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG

AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG

GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC

TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA

AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	100	100	1
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.682	100	0.48
pilA	Acinetobacter baumannii strain A118	45.333	100	0.453
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.575	97.333	0.453
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.675	100	0.447
pilA	Vibrio cholerae C6706	42.675	100	0.447
pilA	Vibrio cholerae strain A1552	42.675	100	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	48.507	89.333	0.433
comP	Acinetobacter baylyi ADP1	40.789	100	0.413
pilA2	Legionella pneumophila strain ERS1305867	39.865	98.667	0.393
pilA/pilA1	Eikenella corrodens VA1	39.456	98	0.387
pilA2	Legionella pneumophila str. Paris	39.189	98.667	0.387

Detailed information NTG0734162