Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PALA13_RS05055 Genome accession   NZ_CP106682
Coordinates   1069535..1070407 (-) Length   290 a.a.
NCBI ID   WP_023087804.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1064535..1075407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA13_RS05010 (PALA13_00990) - 1065396..1065566 (-) 171 WP_003094672.1 DUF3094 family protein -
  PALA13_RS05015 (PALA13_00991) - 1065592..1066041 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  PALA13_RS05020 (PALA13_00992) - 1066038..1066667 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PALA13_RS05025 (PALA13_00993) - 1066800..1067225 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PALA13_RS05030 (PALA13_00994) - 1067222..1067692 (+) 471 WP_003103866.1 hypothetical protein -
  PALA13_RS05035 (PALA13_00995) - 1067689..1068378 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA13_RS05040 (PALA13_00996) - 1068484..1068693 (+) 210 WP_003094660.1 hypothetical protein -
  PALA13_RS05045 (PALA13_00997) yacG 1068730..1068930 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA13_RS05050 (PALA13_00998) coaE 1068927..1069538 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  PALA13_RS05055 (PALA13_00999) pilD 1069535..1070407 (-) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA13_RS05060 (PALA13_01000) pilC 1070411..1071631 (-) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  PALA13_RS05065 (PALA13_01001) pilB 1071635..1073335 (-) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA13_RS05070 pilA 1073564..1074016 (+) 453 WP_024947914.1 pilin Machinery gene
  PALA13_RS05080 (PALA13_01003) nadC 1074204..1075052 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31869.92 Da        Isoelectric Point: 7.7068

>NTDB_id=734159 PALA13_RS05055 WP_023087804.1 1069535..1070407(-) (pilD) [Pseudomonas aeruginosa strain PALA13]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=734159 PALA13_RS05055 WP_023087804.1 1069535..1070407(-) (pilD) [Pseudomonas aeruginosa strain PALA13]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469