Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   PKHYL_RS17935 Genome accession   NZ_AP019516
Coordinates   360647..361099 (+) Length   150 a.a.
NCBI ID   WP_332835917.1    Uniprot ID   -
Organism   Psychrobacter sp. KH172YL61     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355647..366099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKHYL_RS01865 (PKHYL_04320) - 356403..359276 (+) 2874 WP_240365848.1 pilus assembly protein PilY -
  PKHYL_RS01870 (PKHYL_04330) - 359289..359840 (+) 552 WP_240365849.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PKHYL_RS01875 (PKHYL_04340) - 359840..360283 (+) 444 WP_240365850.1 type IV pilin protein -
  PKHYL_RS17935 (PKHYL_04350) pilA/pilA1 360647..361099 (+) 453 WP_332835917.1 pilin Machinery gene
  PKHYL_RS17030 (PKHYL_04360) pilA 361190..361384 (+) 195 WP_269778219.1 pilin Machinery gene
  PKHYL_RS01895 (PKHYL_04370) - 361356..361640 (+) 285 WP_240365851.1 pilin -
  PKHYL_RS01900 (PKHYL_04380) tfpZ 361800..362456 (+) 657 WP_240365852.1 TfpX/TfpZ family type IV pilin accessory protein -
  PKHYL_RS01905 (PKHYL_04390) fdxA 362542..362865 (-) 324 WP_011279554.1 ferredoxin FdxA -
  PKHYL_RS01910 (PKHYL_04400) mutS 362963..366048 (-) 3086 Protein_376 DNA mismatch repair protein MutS -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15093.29 Da        Isoelectric Point: 6.2267

>NTDB_id=72649 PKHYL_RS17935 WP_332835917.1 360647..361099(+) (pilA/pilA1) [Psychrobacter sp. KH172YL61]
MNTVQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQASEGFTATSGLQSDIGVYVADKGALPATADYAAGKPAAPIA
AKALDGKYFAPGGVTVGDKGVITVKFDAGSNSGKAMILEPTNNGGQISGWKCAPAATTGIDASRLPASCQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=72649 PKHYL_RS17935 WP_332835917.1 360647..361099(+) (pilA/pilA1) [Psychrobacter sp. KH172YL61]
ATGAACACTGTACAAAAAGGTTTTACCCTAATCGAACTAATGATCGTGATCGCTATTATCGGTATTCTAGCTGCGATCGC
ACTACCTGCTTATCAAGACTATACAGCTCGTGCACAAGCATCAGAAGGATTCACAGCGACTTCTGGTCTACAAAGTGATA
TAGGGGTCTATGTAGCAGATAAAGGTGCTCTTCCAGCTACTGCCGATTATGCTGCTGGTAAACCTGCTGCACCAATTGCT
GCCAAGGCTTTAGACGGTAAATATTTTGCTCCAGGTGGCGTTACAGTTGGTGATAAAGGCGTCATTACTGTGAAATTTGA
CGCAGGTTCAAATAGTGGTAAGGCTATGATATTGGAACCTACCAATAATGGTGGCCAAATATCAGGCTGGAAGTGTGCGC
CTGCTGCTACTACTGGTATTGATGCTTCACGCTTACCTGCTAGCTGTCAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

64.052

100

0.653
  pilE Neisseria gonorrhoeae strain FA1090

40.719

100

0.453
  pilE Neisseria gonorrhoeae MS11

40.361

100

0.447
  pilA Ralstonia pseudosolanacearum GMI1000

37.791

100

0.433
  comP Acinetobacter baylyi ADP1

41.892

98.667

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.892

98.667

0.413
  pilA2 Legionella pneumophila str. Paris

41.216

98.667

0.407
  pilA Haemophilus influenzae 86-028NP

39.726

97.333

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.926

99.333

0.387
  pilA Haemophilus influenzae Rd KW20

37.838

98.667

0.373
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.286

93.333

0.367
  pilA Vibrio cholerae C6706

39.286

93.333

0.367
  pilA Vibrio cholerae strain A1552

39.286

93.333

0.367

Multiple sequence alignment