Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   MKL45_RS16425 Genome accession   NZ_CP102934
Coordinates   3456419..3456880 (-) Length   153 a.a.
NCBI ID   WP_002062166.1    Uniprot ID   A0A9E9MD50
Organism   Acinetobacter sp. AOR07_HL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3451419..3461880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKL45_RS16400 (MKL45_16405) - 3451780..3452163 (+) 384 WP_000090000.1 RidA family protein -
  MKL45_RS16405 (MKL45_16410) - 3452373..3452567 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  MKL45_RS16410 (MKL45_16415) bfr 3452812..3453276 (+) 465 WP_000678123.1 bacterioferritin -
  MKL45_RS16415 (MKL45_16420) - 3453317..3454945 (-) 1629 WP_002062192.1 O-antigen ligase family protein -
  MKL45_RS16420 (MKL45_16425) - 3455052..3456362 (-) 1311 WP_002062173.1 O-antigen ligase family protein -
  MKL45_RS16425 (MKL45_16430) pilE 3456419..3456880 (-) 462 WP_002062166.1 pilin Machinery gene
  MKL45_RS16430 (MKL45_16435) - 3457225..3457785 (-) 561 WP_000258951.1 TPM domain-containing protein -
  MKL45_RS16435 (MKL45_16440) - 3457779..3458861 (-) 1083 WP_002062201.1 TPM domain-containing protein -
  MKL45_RS16440 (MKL45_16445) - 3458883..3459473 (-) 591 WP_000846931.1 LemA family protein -
  MKL45_RS16445 (MKL45_16450) - 3459604..3460491 (-) 888 WP_030423574.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15655.83 Da        Isoelectric Point: 7.5868

>NTDB_id=719970 MKL45_RS16425 WP_002062166.1 3456419..3456880(-) (pilE) [Acinetobacter sp. AOR07_HL]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQVSEAMELASGLKGAVAETFSQDATCPTNGTNGIATATDINGK
YVASVTTAGNATASGGCTITAKMRATGVSKGIQNGELILTLSNADKGSNVWSCTSTGPNKIEQKYLPKSCTSS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=719970 MKL45_RS16425 WP_002062166.1 3456419..3456880(-) (pilE) [Acinetobacter sp. AOR07_HL]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTTATGATCGTGGTTGCCATTATCGGTATTTTGGCGGCAATTGCGAT
TCCTGCGTATCAAGACTATATCGCTCGTTCTCAAGTTAGTGAAGCTATGGAGTTAGCTAGTGGATTGAAGGGAGCTGTTG
CAGAGACTTTTAGTCAAGATGCTACTTGCCCAACAAATGGTACTAATGGAATTGCTACTGCTACTGATATTAATGGTAAA
TATGTTGCTTCTGTAACCACGGCAGGTAACGCTACTGCCTCAGGTGGATGTACAATTACTGCAAAAATGCGGGCAACTGG
AGTTTCAAAAGGTATTCAAAATGGAGAGCTTATTCTAACTCTTAGTAATGCAGATAAAGGTTCAAATGTGTGGAGTTGTA
CTTCAACTGGTCCAAACAAAATTGAACAAAAGTACTTACCTAAATCATGTACTTCTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

47.826

100

0.503
  pilE Neisseria gonorrhoeae MS11

43.21

100

0.458
  pilA2 Legionella pneumophila strain ERS1305867

43.046

98.693

0.425
  pilA2 Legionella pneumophila str. Paris

43.046

98.693

0.425
  pilA Ralstonia pseudosolanacearum GMI1000

39.752

100

0.418
  pilA Acinetobacter nosocomialis M2

51.2

81.699

0.418
  comP Acinetobacter baylyi ADP1

42.384

98.693

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.105

99.346

0.418
  pilA Acinetobacter baumannii strain A118

39.873

100

0.412
  pilA Pseudomonas aeruginosa PAK

38.462

100

0.392
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.386
  pilA Vibrio parahaemolyticus RIMD 2210633

41.727

90.85

0.379
  pilA Haemophilus influenzae Rd KW20

40.58

90.196

0.366
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.086

98.693

0.366