Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AFERRID_RS05730 Genome accession   NZ_AP018795
Coordinates   1119714..1120856 (-) Length   380 a.a.
NCBI ID   WP_126604553.1    Uniprot ID   A0A2Z6IMA7
Organism   Acidithiobacillus ferridurans strain JCM 18981     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1114714..1125856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFERRID_RS05705 (AFERRID_11350) - 1115093..1115794 (+) 702 WP_113526333.1 Crp/Fnr family transcriptional regulator -
  AFERRID_RS05710 (AFERRID_11360) sdo 1115935..1116624 (+) 690 WP_126604552.1 sulfur dioxygenase subunit alpha Sdo -
  AFERRID_RS05715 (AFERRID_11370) - 1116638..1117255 (+) 618 WP_113526331.1 hypothetical protein -
  AFERRID_RS05720 (AFERRID_11380) - 1117380..1118507 (+) 1128 WP_113526330.1 NAD(P)/FAD-dependent oxidoreductase -
  AFERRID_RS15490 (AFERRID_11390) - 1118546..1119073 (-) 528 WP_331251181.1 hypothetical protein -
  AFERRID_RS15495 (AFERRID_11400) - 1118980..1119708 (-) 729 WP_232027936.1 glycosyltransferase -
  AFERRID_RS05730 (AFERRID_11410) pilU 1119714..1120856 (-) 1143 WP_126604553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AFERRID_RS05735 (AFERRID_11420) - 1120920..1122242 (-) 1323 WP_070807692.1 transposase -
  AFERRID_RS05740 (AFERRID_11440) - 1122412..1123460 (-) 1049 Protein_1127 type IV pilus twitching motility protein PilT -
  AFERRID_RS05745 (AFERRID_11450) - 1123562..1124230 (+) 669 WP_113526326.1 YggS family pyridoxal phosphate-dependent enzyme -
  AFERRID_RS05750 (AFERRID_11460) proC 1124227..1125054 (+) 828 WP_113526325.1 pyrroline-5-carboxylate reductase -
  AFERRID_RS05755 (AFERRID_11470) - 1125051..1125329 (+) 279 WP_113526324.1 YggT family protein -
  AFERRID_RS05760 (AFERRID_11480) - 1125354..1125704 (+) 351 WP_126604554.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42059.52 Da        Isoelectric Point: 5.9990

>NTDB_id=71441 AFERRID_RS05730 WP_126604553.1 1119714..1120856(-) (pilU) [Acidithiobacillus ferridurans strain JCM 18981]
MSKLQSVLDDLLVLMVEKNGSDLYLTVGSPPVMKVDGRTVPLGTEMLKPGQVLNLAKEILGMERLQEFQHEKEINMAISV
PEVGRFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKDLAMTSRGLVLFVGSTGSGKSTSLASMVQYRNQNAAGHI
LTIEDPIEFLHRNAKSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAIAYAETGHLCMSTLHANNANQ
AIERIINFFPEDRKKQLLMDLSLNLRAVVSQRLLPVKGQSGRIAAMEVLINTPAIADLIYKGEVSLLKDAMSRTNDVGMQ
TFDQSLLQLFMDGLVEYDDALRAADSQNDLRLAIKQACMRSGMADPGAATSAEGQWRIQS

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=71441 AFERRID_RS05730 WP_126604553.1 1119714..1120856(-) (pilU) [Acidithiobacillus ferridurans strain JCM 18981]
ATGTCTAAACTTCAGTCTGTACTGGATGACTTGCTGGTCCTGATGGTGGAAAAAAACGGCTCGGACCTGTACCTCACCGT
GGGCTCGCCACCGGTCATGAAAGTCGACGGGCGGACGGTTCCGCTGGGGACAGAAATGCTGAAACCGGGTCAGGTGCTGA
ATCTGGCCAAGGAAATTCTGGGGATGGAGCGCTTACAGGAGTTCCAGCACGAGAAAGAAATCAACATGGCCATTTCCGTG
CCCGAGGTGGGGCGTTTCCGGGTCAACGGGTTTTTCCAGCGTGGTGAACTGAGCTTTGTCCTGCGCGCCATCAAGACCCA
GATTCCCAGCCTGGAGCAGCTCAGGCTGCCGCCGATACTGAAAGATCTGGCCATGACGTCGCGGGGGCTGGTGCTCTTCG
TCGGTTCTACCGGTTCCGGGAAAAGCACCTCCCTGGCGTCGATGGTCCAGTATCGCAACCAGAATGCGGCGGGTCATATC
CTCACCATTGAGGATCCAATCGAATTTCTGCACCGGAATGCGAAGTCCATCGTCAATCAGCGCGAAGTGGGCATCGATAC
CCTGAATTATGAGCGGGCGCTGGAGAATGCGTTGCGGGAAGCGCCGGATGTCATCCTGATCGGCGAAGTGCGCAGCCGGG
ACACCATGGATCATGCCATCGCTTATGCCGAAACCGGGCACCTGTGTATGTCCACCCTCCACGCGAACAATGCCAATCAG
GCCATTGAACGGATCATCAATTTTTTCCCGGAAGATCGTAAAAAACAGTTGCTCATGGATCTTTCACTCAATTTGCGTGC
CGTGGTCTCACAGCGCCTGTTGCCGGTCAAGGGGCAGAGCGGACGAATCGCTGCCATGGAGGTGCTGATCAATACCCCGG
CCATTGCCGATCTGATCTACAAGGGTGAAGTGAGTCTGCTGAAGGACGCCATGTCGCGAACGAACGACGTCGGTATGCAG
ACGTTTGATCAGAGCCTGTTGCAGTTGTTTATGGATGGACTGGTGGAATATGACGATGCCCTGCGCGCGGCCGATTCGCA
GAACGATCTGCGCCTCGCCATCAAACAGGCGTGTATGCGTTCGGGCATGGCCGATCCTGGGGCCGCCACCTCTGCGGAAG
GGCAGTGGCGTATTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6IMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.908

91.579

0.539
  pilU Acinetobacter baylyi ADP1

53.868

91.842

0.495
  pilU Vibrio cholerae strain A1552

53.736

91.579

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.939

91.316

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.954

86.053

0.387
  pilT Vibrio cholerae strain A1552

44.954

86.053

0.387
  pilT Acinetobacter baylyi ADP1

42.183

89.211

0.376
  pilT Acinetobacter baumannii D1279779

41.543

88.684

0.368
  pilT Acinetobacter nosocomialis M2

41.543

88.684

0.368
  pilT Acinetobacter baumannii strain A118

41.543

88.684

0.368
  pilT Legionella pneumophila strain Lp02

41.791

88.158

0.368
  pilT Legionella pneumophila strain ERS1305867

41.791

88.158

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.95

88.684

0.363
  pilT Pseudomonas aeruginosa PAK

40.95

88.684

0.363

Multiple sequence alignment