Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NNL25_RS24000 Genome accession   NZ_CP101540
Coordinates   5155410..5155862 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain D-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5150410..5160862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL25_RS23980 (NNL25_23950) ampD 5151116..5151682 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NNL25_RS23985 (NNL25_23955) - 5151833..5154088 (-) 2256 WP_009315900.1 DUF1631 domain-containing protein -
  NNL25_RS23990 (NNL25_23960) nadC 5154374..5155222 (+) 849 WP_255866345.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NNL25_RS24000 (NNL25_23970) pilA 5155410..5155862 (-) 453 WP_024947914.1 pilin Machinery gene
  NNL25_RS24005 (NNL25_23975) pilB 5156091..5157791 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NNL25_RS24010 (NNL25_23980) pilC 5157795..5159015 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  NNL25_RS24015 (NNL25_23985) pilD 5159019..5159891 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NNL25_RS24020 (NNL25_23990) coaE 5159888..5160499 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  NNL25_RS24025 (NNL25_23995) yacG 5160496..5160696 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=710541 NNL25_RS24000 WP_024947914.1 5155410..5155862(-) (pilA) [Pseudomonas aeruginosa strain D-2]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=710541 NNL25_RS24000 WP_024947914.1 5155410..5155862(-) (pilA) [Pseudomonas aeruginosa strain D-2]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387