Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NNL25_RS24010 Genome accession   NZ_CP101540
Coordinates   5157795..5159015 (+) Length   406 a.a.
NCBI ID   WP_016253888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain D-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5152795..5164015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL25_RS23990 (NNL25_23960) nadC 5154374..5155222 (+) 849 WP_255866345.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NNL25_RS24000 (NNL25_23970) pilA 5155410..5155862 (-) 453 WP_024947914.1 pilin Machinery gene
  NNL25_RS24005 (NNL25_23975) pilB 5156091..5157791 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NNL25_RS24010 (NNL25_23980) pilC 5157795..5159015 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  NNL25_RS24015 (NNL25_23985) pilD 5159019..5159891 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NNL25_RS24020 (NNL25_23990) coaE 5159888..5160499 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  NNL25_RS24025 (NNL25_23995) yacG 5160496..5160696 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  NNL25_RS24030 (NNL25_24000) - 5160733..5160942 (-) 210 WP_003094660.1 hypothetical protein -
  NNL25_RS24035 (NNL25_24005) - 5161048..5161737 (-) 690 WP_023119820.1 energy-coupling factor ABC transporter permease -
  NNL25_RS24040 (NNL25_24010) - 5161734..5162204 (-) 471 WP_003094664.1 hypothetical protein -
  NNL25_RS24045 (NNL25_24015) - 5162201..5162626 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  NNL25_RS24050 (NNL25_24020) - 5162759..5163388 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  NNL25_RS24055 (NNL25_24025) - 5163385..5163834 (+) 450 WP_003161439.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44549.37 Da        Isoelectric Point: 9.6983

>NTDB_id=710543 NNL25_RS24010 WP_016253888.1 5157795..5159015(+) (pilC) [Pseudomonas aeruginosa strain D-2]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFEGFGAELPAFTQMIVNLSEFMQEWWFFIILAIAIFGFAFK
ELHKRSQKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=710543 NNL25_RS24010 WP_016253888.1 5157795..5159015(+) (pilC) [Pseudomonas aeruginosa strain D-2]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAAGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAGGGCTTCGACAACCCCAACATGCGCAAGCTTGTGGATGA
AATCAAACAGGAAGTTTCCTCAGGTAACAGCCTAGCCAACTCCTTGAGAAAAAAGCCCCAGTATTTTGACGAGCTTTATT
GCAACCTGGTAGATGCAGGGGAACAGTCTGGCGCCTTGGAAAACCTTCTCGATCGGGTGGCAACCTATAAAGAAAAGACG
GAATCACTGAAAGCCAAGATCAAAAAGGCGATGACCTATCCCATTGCCGTCATCATTGTCGCACTGATTGTATCTGCGAT
CCTCCTGATTAAAGTGGTTCCACAATTTCAGTCGGTCTTTGAAGGTTTCGGCGCGGAACTTCCCGCCTTTACCCAGATGA
TTGTCAATCTATCGGAGTTCATGCAGGAGTGGTGGTTCTTCATCATACTGGCGATAGCGATATTTGGCTTTGCATTCAAA
GAATTGCATAAACGCTCACAAAAATTCCGTGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.543

99.754

0.764
  pilC Acinetobacter baumannii D1279779

61.386

99.507

0.611
  pilC Acinetobacter baylyi ADP1

60.837

100

0.608
  pilC Legionella pneumophila strain ERS1305867

55.051

97.537

0.537
  pilG Neisseria gonorrhoeae MS11

46.173

99.754

0.461
  pilG Neisseria meningitidis 44/76-A

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411
  pilC Thermus thermophilus HB27

36.908

98.768

0.365