Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMB96_RS09755 Genome accession   NZ_CP101417
Coordinates   2307703..2308836 (-) Length   377 a.a.
NCBI ID   WP_006449645.1    Uniprot ID   A0A0G8M6N0
Organism   Xanthomonas hortorum strain jj2001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2302703..2313836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB96_RS09740 (NMB96_09725) - 2303706..2305094 (-) 1389 WP_006450465.1 DEAD/DEAH box helicase -
  NMB96_RS09745 (NMB96_09730) - 2305403..2306461 (+) 1059 WP_255086951.1 fumarylacetoacetate hydrolase family protein -
  NMB96_RS09750 (NMB96_09735) maiA 2306517..2307179 (+) 663 WP_255086777.1 maleylacetoacetate isomerase -
  NMB96_RS09755 (NMB96_09740) pilU 2307703..2308836 (-) 1134 WP_006449645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMB96_RS09760 (NMB96_09745) - 2308935..2309318 (+) 384 WP_006449646.1 DUF4398 domain-containing protein -
  NMB96_RS09765 (NMB96_09750) - 2309315..2310205 (+) 891 WP_255086778.1 hypothetical protein -
  NMB96_RS09770 (NMB96_09755) - 2310542..2310760 (+) 219 WP_002814034.1 YdcH family protein -
  NMB96_RS09775 (NMB96_09760) - 2310979..2312349 (+) 1371 WP_074056614.1 pyridoxal-phosphate dependent enzyme -
  NMB96_RS09780 (NMB96_09765) - 2312452..2313648 (+) 1197 WP_255086780.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41755.01 Da        Isoelectric Point: 6.1395

>NTDB_id=710093 NMB96_RS09755 WP_006449645.1 2307703..2308836(-) (pilU) [Xanthomonas hortorum strain jj2001]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAISLPDAGR
FRVNVFKQRGEAGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLLVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGQDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=710093 NMB96_RS09755 WP_006449645.1 2307703..2308836(-) (pilU) [Xanthomonas hortorum strain jj2001]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTTTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAACTCAACATGGCCATCTCGCTGCCCGACGCCGGACGC
TTCCGTGTGAACGTGTTCAAGCAGCGCGGCGAAGCAGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAACTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGCCTGGTGCTGCTGGTCGGCTCGACCGGTT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACCCACACCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CGATTGCATTTGCCGAAACCGGTCATCTGTGTCTGGCGACGCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATT
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATTTGCGTGCGGTGGTCTCCCAGCG
CCTGGTCAAGGGCCAGGACGGACGCCGCCGCCCCGCCACCGAAGTGTTGCTCAACACCCCAATGATTCGCGACCTACTGC
GACGCGGGCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCAGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGCGATGGTCTGGCACTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGTGAGCACGACCCGTACGCGGATTACGAAACGGCAGCAGCGCCCAAGATTAGCC
ACGGGTTTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8M6N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.925

95.225

0.504
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.455

100

0.44
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Neisseria gonorrhoeae MS11

39.655

92.308

0.366
  pilT Neisseria meningitidis 8013

39.655

92.308

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366