Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL778_RS01705 Genome accession   NZ_CP100652
Coordinates   361138..362136 (+) Length   332 a.a.
NCBI ID   WP_012312315.1    Uniprot ID   -
Organism   Pseudomonas putida strain HSM-C2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356138..367136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL778_RS01675 (NL778_01675) - 356615..357049 (-) 435 WP_012312309.1 DUF4426 domain-containing protein -
  NL778_RS01680 (NL778_01680) metW 357072..357692 (-) 621 WP_012312310.1 methionine biosynthesis protein MetW -
  NL778_RS01685 (NL778_01685) - 357700..358839 (-) 1140 WP_254467021.1 homoserine O-acetyltransferase -
  NL778_RS01690 (NL778_01690) - 358928..359518 (-) 591 WP_012312312.1 YggT family protein -
  NL778_RS01695 (NL778_01695) proC 359529..360347 (-) 819 WP_012312313.1 pyrroline-5-carboxylate reductase -
  NL778_RS01700 (NL778_01700) - 360386..361072 (-) 687 WP_046787042.1 YggS family pyridoxal phosphate-dependent enzyme -
  NL778_RS01705 (NL778_01705) pilT 361138..362136 (+) 999 WP_012312315.1 type IV pilus twitching motility protein PilT Machinery gene
  NL778_RS01710 (NL778_01710) - 362173..362799 (-) 627 WP_012312316.1 C40 family peptidase -
  NL778_RS01715 (NL778_01715) - 362976..363395 (+) 420 WP_012312317.1 TM2 domain-containing protein -
  NL778_RS01720 (NL778_01720) - 363440..364138 (-) 699 WP_012312318.1 SPOR domain-containing protein -
  NL778_RS01725 (NL778_01725) argS 364140..365876 (-) 1737 WP_012312319.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35949.46 Da        Isoelectric Point: 6.8540

>NTDB_id=706050 NL778_RS01705 WP_012312315.1 361138..362136(+) (pilT) [Pseudomonas putida strain HSM-C2]
MDVTDLLARAGDAGASDLHLAAGEIPMLRVDGELRRMALPSVTPVALSDALAPLLDEGQRQRWAQGDEQDLALDLPSLGR
FRLNLFRQLNGPAATFRLIPKRIATLDELGLEDVFRSVAHWTDGLILVGGPTGSGKSSTMATLIDQLNRQRSLHIITLED
PVEVIHSSERSLVNQREIGRHCRDFSQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSVDRL
VEVFAAEEKPLMRAMLAESLRMVVAQVLVKGVGGGRVAAREVLVATPAVRNLIREGRMAQLNSVMQTGAAQGMRTMEGAL
QRLKTEGLIGAV

Nucleotide


Download         Length: 999 bp        

>NTDB_id=706050 NL778_RS01705 WP_012312315.1 361138..362136(+) (pilT) [Pseudomonas putida strain HSM-C2]
ATGGACGTGACTGACCTGTTGGCCCGGGCTGGCGATGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGAGATACCAAT
GCTACGCGTGGATGGCGAACTGCGGCGCATGGCGCTACCGAGCGTGACGCCCGTGGCGTTGAGCGATGCCCTGGCGCCGC
TGCTCGATGAGGGCCAGCGCCAGCGGTGGGCGCAAGGTGATGAGCAGGACCTGGCACTGGATCTGCCCTCGCTCGGGCGC
TTTCGTCTGAACCTCTTTCGCCAGTTGAATGGCCCGGCGGCCACCTTTCGCTTGATCCCGAAGCGGATTGCGACACTCGA
CGAGCTCGGCCTGGAAGATGTGTTTCGGTCTGTTGCACATTGGACTGACGGTCTGATTCTGGTCGGCGGGCCTACCGGCA
GCGGTAAATCCAGCACTATGGCTACCCTGATCGATCAGTTGAACCGCCAGCGCTCCTTGCACATCATCACCCTCGAAGAC
CCAGTCGAAGTTATCCACAGCAGCGAGCGAAGCCTTGTCAATCAGCGTGAGATCGGTCGGCACTGCCGTGACTTTTCGCA
AGGGTTGCGCAGCGCGCTGCGTCAGGACCCAGATGTGATCGTCATAGGCGAATTGCGCGACCTGGAGACGATTCGCCTGG
CGTTGCGGGCCGCGGAGACCGGGCACCTGGTGCTGGCAACGGTGCATACGCGCTCGGCTGCTGGCAGTGTCGACCGGCTG
GTGGAAGTGTTTGCCGCCGAGGAGAAACCGCTGATGCGGGCGATGTTGGCGGAGTCACTGCGTATGGTGGTTGCACAGGT
GCTGGTCAAGGGTGTGGGGGGTGGGCGGGTGGCCGCCCGGGAGGTGCTGGTGGCTACGCCTGCGGTGCGCAACCTGATCC
GCGAGGGGCGTATGGCACAATTGAATTCGGTGATGCAGACGGGGGCTGCGCAAGGAATGCGGACGATGGAGGGGGCGTTG
CAGCGATTGAAGACCGAAGGTTTGATCGGGGCCGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.672

99.096

0.512
  pilT Pseudomonas stutzeri DSM 10701

51.368

99.096

0.509
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Neisseria meningitidis 8013

50.453

99.699

0.503
  pilT Neisseria gonorrhoeae MS11

50.151

99.699

0.5
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Acinetobacter nosocomialis M2

50.152

99.096

0.497
  pilT Vibrio cholerae strain A1552

49.547

99.699

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.547

99.699

0.494
  pilT Legionella pneumophila strain Lp02

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.788

99.398

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.045

100

0.452
  pilU Pseudomonas stutzeri DSM 10701

36.97

99.398

0.367