Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NKJ47_RS06480 Genome accession   NZ_CP100647
Coordinates   1589687..1590079 (+) Length   130 a.a.
NCBI ID   WP_254460682.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain DD13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1590642..1610303 1589687..1590079 flank 563


Gene organization within MGE regions


Location: 1589687..1610303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKJ47_RS06480 pilA/pilAI 1589687..1590079 (+) 393 WP_254460682.1 pilin Machinery gene
  NKJ47_RS06490 - 1590184..1590609 (+) 426 WP_302329810.1 pilin -
  NKJ47_RS06495 - 1590642..1592474 (+) 1833 WP_254460683.1 hypothetical protein -
  NKJ47_RS06500 - 1592534..1593742 (-) 1209 WP_254460684.1 glycosyltransferase -
  NKJ47_RS06505 - 1593739..1594689 (-) 951 WP_254460685.1 YbhN family protein -
  NKJ47_RS06510 - 1594686..1595474 (-) 789 WP_254460686.1 class I SAM-dependent methyltransferase -
  NKJ47_RS06515 - 1595497..1596354 (-) 858 WP_254460687.1 glycosyltransferase family 2 protein -
  NKJ47_RS06520 pilB 1596488..1598227 (+) 1740 WP_254460688.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NKJ47_RS06525 pilR 1598474..1599862 (-) 1389 WP_254460689.1 sigma-54 dependent transcriptional regulator Regulator
  NKJ47_RS06530 - 1599945..1601558 (-) 1614 WP_254460690.1 ATP-binding protein -
  NKJ47_RS06535 sucC 1601940..1603109 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -
  NKJ47_RS06540 sucD 1603132..1604007 (+) 876 WP_010342226.1 succinate--CoA ligase subunit alpha -
  NKJ47_RS06545 - 1604208..1605485 (-) 1278 WP_254460691.1 DUF2254 domain-containing protein -
  NKJ47_RS06550 - 1605737..1607833 (+) 2097 WP_254460692.1 pyrroloquinoline quinone-dependent dehydrogenase -
  NKJ47_RS06555 - 1608526..1610163 (+) 1638 WP_254460693.1 NAD+ synthase -

Sequence

Protein


Download         Length: 130 a.a.        Molecular weight: 13561.76 Da        Isoelectric Point: 9.5775

>NTDB_id=705946 NKJ47_RS06480 WP_254460682.1 1589687..1590079(+) (pilA/pilAI) [Xanthomonas sacchari strain DD13]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQFEVKKNEGSTLSTPAQIGLAASTTRCTI
TVTDTTIACTLKGNAAKIANKNVTLTRTASTGTWACSSSVDAKYKPTGCS

Nucleotide


Download         Length: 393 bp        

>NTDB_id=705946 NKJ47_RS06480 WP_254460682.1 1589687..1590079(+) (pilA/pilAI) [Xanthomonas sacchari strain DD13]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTTGCGATCATCGCCATCCTGGCCGCCATCGCGTT
GCCGATGTACCAGGACTACGTTGCCAAGTCGCAGGCCACCGCTGGTCTGGCGGAAATCACCCCGGGCAAGACGCAGTTCG
AGGTGAAGAAGAACGAAGGCAGTACCCTCAGCACGCCGGCCCAGATCGGTCTGGCTGCAAGCACGACCCGTTGCACCATC
ACCGTGACCGACACCACGATTGCCTGCACCCTGAAGGGCAATGCTGCGAAGATCGCAAACAAGAACGTCACCCTGACCCG
TACCGCCAGCACCGGCACCTGGGCTTGCTCCTCGTCGGTGGATGCCAAGTACAAGCCGACCGGCTGCAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-129)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

57.778

100

0.6
  pilA Acinetobacter baumannii strain A118

48.921

100

0.523
  pilA/pilAII Pseudomonas stutzeri DSM 10701

49.624

100

0.508
  pilA Pseudomonas aeruginosa PAK

44.295

100

0.508
  pilA Vibrio cholerae C6706

44.138

100

0.492
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.138

100

0.492
  pilA Vibrio cholerae strain A1552

44.138

100

0.492
  pilA2 Legionella pneumophila str. Paris

37.908

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

36.913

100

0.423
  comP Acinetobacter baylyi ADP1

37.931

100

0.423
  pilA Vibrio parahaemolyticus RIMD 2210633

42.4

96.154

0.408
  pilA2 Legionella pneumophila strain ERS1305867

35.862

100

0.4
  pilA Haemophilus influenzae 86-028NP

42.017

91.538

0.385