Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NH673_RS05705 Genome accession   NZ_CP099727
Coordinates   1270224..1271222 (-) Length   332 a.a.
NCBI ID   WP_014592720.1    Uniprot ID   -
Organism   Pseudomonas putida strain PCL1760     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1265224..1276222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH673_RS05685 (NH673_05685) argS 1266371..1268107 (+) 1737 WP_019439134.1 arginine--tRNA ligase -
  NH673_RS05690 (NH673_05690) - 1268109..1268807 (+) 699 WP_014592717.1 SPOR domain-containing protein -
  NH673_RS05695 (NH673_05695) - 1268898..1269317 (-) 420 WP_014592718.1 TM2 domain-containing protein -
  NH673_RS05700 (NH673_05700) - 1269484..1270107 (+) 624 WP_053867807.1 C40 family peptidase -
  NH673_RS05705 (NH673_05705) pilT 1270224..1271222 (-) 999 WP_014592720.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NH673_RS05710 (NH673_05710) - 1271288..1271974 (+) 687 WP_019439137.1 YggS family pyridoxal phosphate-dependent enzyme -
  NH673_RS05715 (NH673_05715) proC 1272013..1272831 (+) 819 WP_019439138.1 pyrroline-5-carboxylate reductase -
  NH673_RS05720 (NH673_05720) - 1272842..1273432 (+) 591 WP_003249110.1 YggT family protein -
  NH673_RS05725 (NH673_05725) - 1273532..1274671 (+) 1140 WP_053867808.1 homoserine O-acetyltransferase -
  NH673_RS05730 (NH673_05730) metW 1274679..1275299 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  NH673_RS05735 (NH673_05735) - 1275322..1275756 (+) 435 WP_019439139.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36165.85 Da        Isoelectric Point: 7.4427

>NTDB_id=701340 NH673_RS05705 WP_014592720.1 1270224..1271222(-) (pilT) [Pseudomonas putida strain PCL1760]
MDVTDLLARAVDAGASDLHLATGQIPMLRLDGELQRMGLPPLSQGALIEGMASVLDDDQRRQWAQDDEMDLALELPALGR
FRLNLFRQLNGPSVTFRLIPGRIATVSELDLVDVFQSVAQCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEVIHASQRSLVNQREMGRHSRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSATSCIDRL
VEVFAAEEKPLVRAMLAESLRLVVTQVLVRRVGGGRVAAREVLVATPAVRNLVREGRLAQLSSVMQGGAAEGMRTMEGAL
RRLRERGLIKGL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=701340 NH673_RS05705 WP_014592720.1 1270224..1271222(-) (pilT) [Pseudomonas putida strain PCL1760]
ATGGATGTGACCGACCTGTTGGCCCGAGCTGTGGATGCGGGGGCTTCAGACCTGCACCTGGCGACGGGCCAGATTCCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGTATGGGCTTGCCGCCCTTGAGCCAGGGGGCCTTGATCGAGGGCATGGCGAGCG
TGCTGGATGACGACCAGCGCCGGCAGTGGGCCCAAGACGATGAAATGGACCTTGCGCTGGAATTGCCGGCGCTTGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGAATGGCCCGTCGGTTACCTTTCGGTTGATTCCGGGGCGCATTGCGACGGTGAG
TGAGCTGGACCTTGTGGATGTGTTTCAATCTGTTGCGCAATGCCGAGATGGTTTGATCCTCGTGGGCGGGCCGACCGGCA
GTGGCAAGTCCAGTACCCTGGCAACGCTGCTCGACCAGCTGAACCGCGAGCGGGCACTGCACATCATTACCCTGGAGGAC
CCTGTCGAAGTCATCCATGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATGGGGCGCCATAGTCGCGGCTTTGCTCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CCCTGCGCGCGGCCGAAACCGGGCACCTGGTGCTGGCAACCGTGCACGCGCGTTCGGCGACCAGCTGCATCGACAGGTTG
GTGGAGGTTTTTGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTCACGCAGGT
GCTGGTCAGGCGCGTGGGGGGTGGGCGGGTGGCTGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGTTGGCGCAGTTGTCTTCGGTGATGCAGGGCGGGGCAGCGGAGGGGATGCGGACGATGGAGGGGGCGTTG
CGGAGGCTGAGGGAGCGAGGGCTGATCAAAGGCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Acinetobacter baumannii D1279779

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.456

99.096

0.5
  pilT Acinetobacter nosocomialis M2

50.456

99.096

0.5
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.096

0.497
  pilT Acinetobacter baylyi ADP1

50

99.398

0.497
  pilT Neisseria meningitidis 8013

49.091

99.398

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria gonorrhoeae MS11

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.328

99.096

0.479
  pilT Legionella pneumophila strain Lp02

48.328

99.096

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.429

100

0.47
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373