Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NG836_RS02215 Genome accession   NZ_CP099575
Coordinates   517070..518059 (-) Length   329 a.a.
NCBI ID   WP_252826131.1    Uniprot ID   -
Organism   Pseudomonas kermanshahensis strain F8     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512070..523059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG836_RS02195 (NG836_02195) argS 513196..514932 (+) 1737 WP_099430684.1 arginine--tRNA ligase -
  NG836_RS02200 (NG836_02200) - 514934..515632 (+) 699 WP_027592346.1 SPOR domain-containing protein -
  NG836_RS02205 (NG836_02205) - 515744..516163 (-) 420 WP_085272247.1 NINE protein -
  NG836_RS02210 (NG836_02210) - 516331..516957 (+) 627 WP_027592344.1 C40 family peptidase -
  NG836_RS02215 (NG836_02215) pilT 517070..518059 (-) 990 WP_252826131.1 type IV pilus twitching motility protein PilT Machinery gene
  NG836_RS02220 (NG836_02220) - 518125..518811 (+) 687 WP_099455618.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG836_RS02225 (NG836_02225) proC 518850..519668 (+) 819 WP_252826132.1 pyrroline-5-carboxylate reductase -
  NG836_RS02230 (NG836_02230) - 519679..520269 (+) 591 WP_027592340.1 YggT family protein -
  NG836_RS02235 (NG836_02235) metX 520373..521512 (+) 1140 WP_099430689.1 homoserine O-succinyltransferase MetX -
  NG836_RS02240 (NG836_02240) metW 521520..522140 (+) 621 WP_008092977.1 methionine biosynthesis protein MetW -
  NG836_RS02245 (NG836_02245) - 522163..522597 (+) 435 WP_252826133.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 35604.26 Da        Isoelectric Point: 5.9821

>NTDB_id=700743 NG836_RS02215 WP_252826131.1 517070..518059(-) (pilT) [Pseudomonas kermanshahensis strain F8]
MDVTDLLARAVDARASDLHLAAGQVPMLRLDGELQRMDLPPLTAAALSDAMAPLLGDDACRQWAQGDELDLALALPALGR
FRLNLFRQLNGVAATFRLIPRRIATLDELDLVEVFQAVAQCSDGLILVGGPTGSGKSSTLAALLDQLNHERPLHIITLED
PVEVIHSSQRSLVNQREMGRDCRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSIDRL
VEVFAAEEKPLVRAMLAESLRVVVAQVLVKRVGGGRVAAREVLVANTAVRNLIREGRMAQLSSVMQTGAAEGMRTMEGAM
QRLEVMGWV

Nucleotide


Download         Length: 990 bp        

>NTDB_id=700743 NG836_RS02215 WP_252826131.1 517070..518059(-) (pilT) [Pseudomonas kermanshahensis strain F8]
ATGGATGTGACCGACCTGTTGGCCCGTGCTGTGGATGCGCGGGCTTCGGACCTGCACCTGGCCGCGGGCCAGGTACCGAT
GCTGCGCCTGGATGGCGAGCTGCAACGCATGGACCTGCCGCCGCTGACGGCAGCGGCGTTGAGTGATGCCATGGCGCCCT
TGCTGGGCGATGACGCTTGCCGGCAATGGGCGCAGGGGGATGAGCTGGACCTTGCGTTGGCGCTGCCAGCGTTAGGCCGT
TTTCGCCTGAACCTGTTCCGCCAGTTGAATGGGGTTGCGGCGACCTTTCGCCTGATACCCAGACGCATTGCGACACTGGA
CGAGCTCGACCTTGTGGAAGTGTTTCAAGCTGTTGCGCAGTGTTCCGATGGCCTGATTCTGGTGGGCGGGCCCACCGGCA
GTGGCAAGTCCAGTACCTTGGCGGCGCTGCTCGATCAGCTCAACCACGAGCGGCCACTGCATATCATCACCCTTGAAGAC
CCTGTGGAAGTTATCCATAGCAGCCAACGGAGCCTGGTCAACCAGCGCGAGATGGGCCGCGATTGCCGCGGGTTTGCGCA
AGGGCTGCGCAGTGCGCTGCGTCAGGACCCGGATGTGATCATGATCGGCGAGTTGCGCGACCTGGAGACGATTCGCTTGG
CATTACGGGCGGCTGAAACGGGGCACTTGGTGCTGGCAACCGTGCACACGCGCTCGGCTGCGGGCAGCATCGACCGGCTG
GTGGAGGTGTTTGCCGCCGAGGAAAAGCCCCTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCGTGGTGGTGGCGCAAGT
GCTGGTCAAGCGCGTGGGCGGCGGGCGGGTGGCGGCGCGGGAGGTGTTGGTGGCTAACACGGCAGTGCGTAACCTGATTC
GCGAAGGGCGGATGGCGCAGCTGAGTTCGGTGATGCAGACGGGGGCGGCGGAGGGGATGCGGACGATGGAAGGGGCGATG
CAAAGGCTGGAAGTAATGGGATGGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.672

100

0.517
  pilT Pseudomonas aeruginosa PAK

51.368

100

0.514
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.514
  pilT Vibrio cholerae strain A1552

51.368

100

0.514
  pilT Acinetobacter baumannii D1279779

50.76

100

0.508
  pilT Acinetobacter baumannii strain A118

50.76

100

0.508
  pilT Acinetobacter nosocomialis M2

50.76

100

0.508
  pilT Pseudomonas stutzeri DSM 10701

50.152

100

0.502
  pilT Neisseria meningitidis 8013

49.394

100

0.495
  pilT Legionella pneumophila strain Lp02

50.155

98.176

0.492
  pilT Legionella pneumophila strain ERS1305867

50.155

98.176

0.492
  pilT Neisseria gonorrhoeae MS11

49.091

100

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.799

96.657

0.462
  pilU Vibrio cholerae strain A1552

36.503

99.088

0.362